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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
09/12/2019 |
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Data da última atualização: |
10/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINEZ, A. F. C.; MELLO, F. M. P.; ZUCCHI, T. D.; MELO, I. S. de; MORAES, L. A. B. |
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Afiliação: |
ANA FLÁVIA CANOVAS MARTINEZ, FFCLRP-USP; FLÁVIA MADOLESI PEREIRA MELLO; TIAGO DOMINGUES ZUCCHI; ITAMAR SOARES DE MELO, CNPMA; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP. |
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Título: |
Tandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by Streptomyces sp. 39 PL. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Natural Product Research, v. 34, n. 2, p. 210-216, 2019. |
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DOI: |
https://doi.org/10.1080/14786419.2018.1525713 |
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Idioma: |
Inglês |
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Notas: |
Arquivo com texto completo contém a versão publicada online, sem dados de volume e paginação. |
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Conteúdo: |
Abstract: Natural products isolated by microorganisms are interesting in the search for new compounds with several biological activities. However, low concentration and structural diversity make the isolation a time-consuming step. Tandem mass spectrometry is a well-established technology for the identification and characterization of target microbial natural products due to high sensitivity and selectivity of these experiments. We developed a method employing neutral loss experiments (LC-ESI-MS/MS) to identify luminacins in microbial crude extracts. The luminacins class exhibited conserved fragmentation pattern with loss at 172Da relative to glycosides fragment and this loss was used in searching for compounds belonging to this class. Therefore, the crude extract produced by Streptomyces sp. 39 PL was analysed and five luminacins were isolated - one is a novel luminacin I at 466Da. |
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Palavras-Chave: |
Dereplication; Luminacins; Neutral loss experiments. |
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Thesagro: |
Análise Química; Bactéria; Identificação; Método de Análise. |
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Thesaurus Nal: |
phytotoxins; Species identification; Streptomyces; Tandem mass spectrometry. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02000naa a2200325 a 4500
001 2116318
005 2022-06-10
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/14786419.2018.1525713$2DOI
100 1 $aMARTINEZ, A. F. C.
245 $aTandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by Streptomyces sp. 39 PL.$h[electronic resource]
260 $c2019
500 $aArquivo com texto completo contém a versão publicada online, sem dados de volume e paginação.
520 $aAbstract: Natural products isolated by microorganisms are interesting in the search for new compounds with several biological activities. However, low concentration and structural diversity make the isolation a time-consuming step. Tandem mass spectrometry is a well-established technology for the identification and characterization of target microbial natural products due to high sensitivity and selectivity of these experiments. We developed a method employing neutral loss experiments (LC-ESI-MS/MS) to identify luminacins in microbial crude extracts. The luminacins class exhibited conserved fragmentation pattern with loss at 172Da relative to glycosides fragment and this loss was used in searching for compounds belonging to this class. Therefore, the crude extract produced by Streptomyces sp. 39 PL was analysed and five luminacins were isolated - one is a novel luminacin I at 466Da.
650 $aphytotoxins
650 $aSpecies identification
650 $aStreptomyces
650 $aTandem mass spectrometry
650 $aAnálise Química
650 $aBactéria
650 $aIdentificação
650 $aMétodo de Análise
653 $aDereplication
653 $aLuminacins
653 $aNeutral loss experiments
700 1 $aMELLO, F. M. P.
700 1 $aZUCCHI, T. D.
700 1 $aMELO, I. S. de
700 1 $aMORAES, L. A. B.
773 $tNatural Product Research$gv. 34, n. 2, p. 210-216, 2019.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
15/01/2020 |
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Data da última atualização: |
02/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ANDRADE, J. C.; SILVA, A. R. P. DA; SANTOS, A. T. L. DOS; FREITAS, M. A.; MATOS, Y. M. L. S. DE; BRAGA, M. F. B. M.; BEZERRA, C. F.; GONÇALO, M. I. P.; VEGA-GÓMEZ, MARIA CELESTE; ROLÓN, M. S.; CORONEL MOLAS, C. C.; RIBEIRO, P. R. V.; BRITO, E. S. de; COUTINHO, H. D. M. |
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Afiliação: |
JACQUELINE COSMO ANDRADE, Microbiology and Molecular Biology Laboratory, Biological Chemistry Department, Regional University of Cariri; Bioassays Laboratory - LABIO, Federal University of Cariri - UFCA, Brejo Santo; ANA RAQUEL PEREIRA DA SILVA, Microbiology and Molecular Biology Laboratory, Biological Chemistry Department, Regional University of Cariri; ANTONIA THASSYA LUCAS DOS SANTOS, Microbiology and Molecular Biology Laboratory, Biological Chemistry Department, Regional University of Cariri; MARIA AUDILENE FREITAS, Microbiology and Molecular Biology Laboratory, Biological Chemistry Department, Regional University of Cariri; YEDDA MARIA LOBO SOARES DE MATOS, Microbiology and Molecular Biology Laboratory, Biological Chemistry Department, Regional University of Cariri; MARIA FLAVIANA BEZERRA MORAIS BRAGA, Microbiology and Molecular Biology Laboratory, Biological Chemistry Department, Regional University of Cariri; CAMILA FONSECA BEZERRA, Microbiology and Molecular Biology Laboratory, Biological Chemistry Department, Regional University of Cariri; MARIA ISABELI PEREIRA GONÇALO, Bioassays Laboratory - LABIO, Federal University of Cariri - UFCA, Brejo Santo; VEGA-GÓMEZ, M. C., Center for the Development of Scientific Research, Moisés Bertoni Foundation/Díaz Gill Laboratories, Asunción; ROLÓN, M. S., Center for the Development of Scientific Research, Moisés Bertoni Foundation/Díaz Gill Laboratories, Asunción; CATHIA CECILIA CORONEL MOLAS, Center for the Development of Scientific Research, Moisés Bertoni Foundation/Díaz Gill Laboratories, Asunción; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; EDY SOUSA DE BRITO, CNPAT; HENRIQUE DOUGLAS MELO COUTINHO, Microbiology and Molecular Biology Laboratory, Biological Chemistry Department, Regional University of Cariri. |
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Título: |
Chemical composition, antiparasitic and cytotoxic activities of aqueous extracts of Ziziphus joazeiro Mart. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Asian Pacific Journal of Tropical Biomedicine, Haikou, v. 9, n. 5, p. 222-226, May 2019. |
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DOI: |
https://doi.org/10.4103/2221-1691.259003 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Antiepimastigote; Antipromastigote; UPLC-MS-ESI-QTOF. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208898/1/ART19113.pdf
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Marc: |
LEADER 01040naa a2200313 a 4500
001 2118831
005 2020-06-02
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4103/2221-1691.259003$2DOI
100 1 $aANDRADE, J. C.
245 $aChemical composition, antiparasitic and cytotoxic activities of aqueous extracts of Ziziphus joazeiro Mart.$h[electronic resource]
260 $c2019
653 $aAntiepimastigote
653 $aAntipromastigote
653 $aUPLC-MS-ESI-QTOF
700 1 $aSILVA, A. R. P. DA
700 1 $aSANTOS, A. T. L. DOS
700 1 $aFREITAS, M. A.
700 1 $aMATOS, Y. M. L. S. DE
700 1 $aBRAGA, M. F. B. M.
700 1 $aBEZERRA, C. F.
700 1 $aGONÇALO, M. I. P.
700 1 $aVEGA-GÓMEZ, MARIA CELESTE
700 1 $aROLÓN, M. S.
700 1 $aCORONEL MOLAS, C. C.
700 1 $aRIBEIRO, P. R. V.
700 1 $aBRITO, E. S. de
700 1 $aCOUTINHO, H. D. M.
773 $tAsian Pacific Journal of Tropical Biomedicine, Haikou$gv. 9, n. 5, p. 222-226, May 2019.
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Embrapa Agroindústria Tropical (CNPAT) |
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