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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BEZERRA, Y. C. dos A.; MARQUES, J. P. R.; STIPP, L. C. L.; ATTÍLIO, L. B.; ASTUA, J. de F.; MOURÃO FILHO, F. de A. A. |
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Afiliação: |
YANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP. |
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Título: |
How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021. |
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ISSN: |
1806-9967 |
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Idioma: |
Português |
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Conteúdo: |
New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance. |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227820/1/How-to-drive-phloem-gene-expression.pdf
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Marc: |
LEADER 02150naa a2200205 a 4500
001 2136203
005 2021-11-17
008 2021 bl uuuu u00u1 u #d
022 $a1806-9967
100 1 $aBEZERRA, Y. C. dos A.
245 $aHow to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.$h[electronic resource]
260 $c2021
520 $aNew approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.
650 $aFruta Cítrica
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aATTÍLIO, L. B.
700 1 $aASTUA, J. de F.
700 1 $aMOURÃO FILHO, F. de A. A.
773 $tRevista Brasileira de Fruticultura, Jaboticabal$gv. 43, n. 4,e-005, 2021.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 591 | |
| 7. |  | MARQUES, J. B. B. Aspectos geográficos e ambientais. In: MARQUES, J. B. B. Senegal: características de um país africano com o qual o Brasil realiza projetos de cooperação. Bagé: Ed. por Demanda, 2015. p. 23-26. il. p&b| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 8. | | MARQUES, J. B. B. Aspectos históricos e políticos. In: MARQUES, J. B. B. Senegal: características de um país africano com o qual o Brasil realiza projetos de cooperação. Bagé: Ed. por Demanda, 2015. p. 17-22. il. p&b| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 9. | | MARQUES, J. B. B. Agricultura no Senegal. In: MARQUES, J. B. B. Senegal: características de um país africano com o qual o Brasil realiza projetos de cooperação. Bagé: Ed. por Demanda, 2015. p. 38-43. il. p&b| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 10. | | MARQUES, J. R. F. Coleção Biológica de muçuãs. In: ALBUQUERQUE, M. do S. M.; IANELLA, P. (Ed.). Inventário de recursos genéticos animais da Embrapa. Brasília, DF: Embrapa, 2016. p. 77-79.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | MARQUES, J. R. F. Composição e melhoramento genético do rebanho. In: VEIGA, J. B. da (Ed.). Criação de gado leiteiro na Zona Bragantina. Belém, PA: Embrapa Amazônia Oriental, 2006. p. 25-34. (Embrapa Amazônia Oriental. Sistemas de produção, 2).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 15. | | MARQUES, J. B. B. Flora e fauna. In: MARQUES, J. B. B. Senegal: características de um país africano com o qual o Brasil realiza projetos de cooperação. Bagé: Ed. por Demanda, 2015. p. 27-31. il. p&b| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 591 | |
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