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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
01/02/2010 |
Data da última atualização: |
18/03/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARON, L. G.; PIÑEROS, M. A.; GUIMARAES, C. T.; MAGALHAES, J. V. de; PLEIMAN, J. K.; MAO, C.; SHAFF, J.; BELICUAS, S. N. J; KOCHIAN, L. V. |
Afiliação: |
Lyza G. Maron, Cornell University; Miguel A. Piñeros, Cornell University; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; Jennifer K. Pleiman, Cornell University; Chuanzao Mao, Zheijang University; Jon Shaff, Cornell University; SILVIA NETO JARDIM, CNPMS; Leon V. Kochian, Cornell University. |
Título: |
Two functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
The Plant Journal, Oxford, v. 61, n. 5, p. 728-740, 2010. |
DOI: |
10.1111/j.1365-313X.2009.04103.x |
Idioma: |
Inglês |
Conteúdo: |
Crop yields are significantly reduced by aluminum (Al) toxicity on acidic soils, which comprise up to 50% of the world?s arable land. Al-activated release of ligands (such as organic acids) from the roots is a major Al tolerance mechanism in plants. In maize, Al-activated root citrate exudation plays an important role in tolerance. However, maize Al tolerance is a complex trait involving multiple genes and physiological mechanisms. Recently, transporters from the MATE family have been shown to mediate Al-activated citrate exudation in a number of plant species. Here we describe the cloning and characterization of two MATE family members in maize, ZmMATE1 and ZmMATE2, which co-localize to major Al tolerance QTL. Both genes encode plasma membrane proteins that mediate significant anion efflux when expressed in Xenopus oocytes. ZmMATE1 expression is mostly concentrated in root tissues, is up-regulated by Al and is significantly higher in Al-tolerant maize genotypes. In contrast, ZmMATE2 expression is not specifically localized to any particular tissue and does not respond to Al. [14C]-citrate efflux experiments in oocytes demonstrate that ZmMATE1 is a citrate transporter. In addition, ZmMATE1 expression confers a significant increase in Al tolerance in transgenic Arabidopsis. Our data suggests that ZmMATE1 is a functional homolog of the Al tolerance genes recently characterized in sorghum, barley and Arabidopsis, and is likely to underlie the largest maize Al tolerance QTL found on chromosome 6. However, ZmMATE2 most likely does not encode a citrate transporter, and could be involved in a novel Al tolerance mechanism. MenosCrop yields are significantly reduced by aluminum (Al) toxicity on acidic soils, which comprise up to 50% of the world?s arable land. Al-activated release of ligands (such as organic acids) from the roots is a major Al tolerance mechanism in plants. In maize, Al-activated root citrate exudation plays an important role in tolerance. However, maize Al tolerance is a complex trait involving multiple genes and physiological mechanisms. Recently, transporters from the MATE family have been shown to mediate Al-activated citrate exudation in a number of plant species. Here we describe the cloning and characterization of two MATE family members in maize, ZmMATE1 and ZmMATE2, which co-localize to major Al tolerance QTL. Both genes encode plasma membrane proteins that mediate significant anion efflux when expressed in Xenopus oocytes. ZmMATE1 expression is mostly concentrated in root tissues, is up-regulated by Al and is significantly higher in Al-tolerant maize genotypes. In contrast, ZmMATE2 expression is not specifically localized to any particular tissue and does not respond to Al. [14C]-citrate efflux experiments in oocytes demonstrate that ZmMATE1 is a citrate transporter. In addition, ZmMATE1 expression confers a significant increase in Al tolerance in transgenic Arabidopsis. Our data suggests that ZmMATE1 is a functional homolog of the Al tolerance genes recently characterized in sorghum, barley and Arabidopsis, and is likely to underlie the largest maize Al tolerance QTL foun... Mostrar Tudo |
Palavras-Chave: |
Tolerância. |
Thesagro: |
Alumínio; Milho; Zea mays. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02522naa a2200277 a 4500 001 1631894 005 2019-03-18 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1111/j.1365-313X.2009.04103.x$2DOI 100 1 $aMARON, L. G. 245 $aTwo functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize.$h[electronic resource] 260 $c2010 520 $aCrop yields are significantly reduced by aluminum (Al) toxicity on acidic soils, which comprise up to 50% of the world?s arable land. Al-activated release of ligands (such as organic acids) from the roots is a major Al tolerance mechanism in plants. In maize, Al-activated root citrate exudation plays an important role in tolerance. However, maize Al tolerance is a complex trait involving multiple genes and physiological mechanisms. Recently, transporters from the MATE family have been shown to mediate Al-activated citrate exudation in a number of plant species. Here we describe the cloning and characterization of two MATE family members in maize, ZmMATE1 and ZmMATE2, which co-localize to major Al tolerance QTL. Both genes encode plasma membrane proteins that mediate significant anion efflux when expressed in Xenopus oocytes. ZmMATE1 expression is mostly concentrated in root tissues, is up-regulated by Al and is significantly higher in Al-tolerant maize genotypes. In contrast, ZmMATE2 expression is not specifically localized to any particular tissue and does not respond to Al. [14C]-citrate efflux experiments in oocytes demonstrate that ZmMATE1 is a citrate transporter. In addition, ZmMATE1 expression confers a significant increase in Al tolerance in transgenic Arabidopsis. Our data suggests that ZmMATE1 is a functional homolog of the Al tolerance genes recently characterized in sorghum, barley and Arabidopsis, and is likely to underlie the largest maize Al tolerance QTL found on chromosome 6. However, ZmMATE2 most likely does not encode a citrate transporter, and could be involved in a novel Al tolerance mechanism. 650 $aAlumínio 650 $aMilho 650 $aZea mays 653 $aTolerância 700 1 $aPIÑEROS, M. A. 700 1 $aGUIMARAES, C. T. 700 1 $aMAGALHAES, J. V. de 700 1 $aPLEIMAN, J. K. 700 1 $aMAO, C. 700 1 $aSHAFF, J. 700 1 $aBELICUAS, S. N. J 700 1 $aKOCHIAN, L. V. 773 $tThe Plant Journal, Oxford$gv. 61, n. 5, p. 728-740, 2010.
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Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
Data corrente: |
14/03/2000 |
Data da última atualização: |
07/08/2019 |
Autoria: |
ARAUJO, A. E. de. |
Afiliação: |
ALDERI EMIDIO DE ARAUJO. |
Título: |
Ensaios de hidrotermoterapia em sementes de feijoeiro macassar (Vigna unguiculata L. Walp) visando o controle de Xanthomonas e Pseudomonas. |
Ano de publicação: |
1987 |
Fonte/Imprenta: |
Areia: UFPB, 1987. |
Páginas: |
65 p. |
Idioma: |
Português |
Notas: |
Dissertação Mestrado. |
Conteúdo: |
Estudo sobre a presenca de bacterias associadas as sementes de feijao, na Paraiba, e sobre o metodo de hidrotermoterapia como tratamento alternativo. Sementes foram coletadas em dez municipios, e tradas a 40°C, 50°C. 60°C e 70°C, em intervalos de 10min em 10min, ate 60min. Um lote nao recebeu tratamento (testemunha absoluta). Com base nos resultados, concluiu-se que as sementes utilizadas pelos produtores no Estado, apresentam elevado nivel de infeccao por bactrias, e o tratamento por hidrotermoterapia nao foi eficiente no contrle das bacterias. |
Palavras-Chave: |
Brasil; Diseases; Paraiba; Pesudomona. |
Thesagro: |
Areia; Bactéria; Controle Químico; Doença; Espécie; Feijão de Corda; Semente; Vigna Unguiculata; Xanthomonas Phaseoli. |
Thesaurus NAL: |
seeds. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/200431/1/Id-4451-Ts-Alderi.pdf
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Marc: |
LEADER 01359nam a2200301 a 4500 001 1668664 005 2019-08-07 008 1987 bl uuuu m 00u1 u #d 100 1 $aARAUJO, A. E. de 245 $aEnsaios de hidrotermoterapia em sementes de feijoeiro macassar (Vigna unguiculata L. Walp) visando o controle de Xanthomonas e Pseudomonas. 260 $aAreia: UFPB$c1987 300 $a65 p. 500 $aDissertação Mestrado. 520 $aEstudo sobre a presenca de bacterias associadas as sementes de feijao, na Paraiba, e sobre o metodo de hidrotermoterapia como tratamento alternativo. Sementes foram coletadas em dez municipios, e tradas a 40°C, 50°C. 60°C e 70°C, em intervalos de 10min em 10min, ate 60min. Um lote nao recebeu tratamento (testemunha absoluta). Com base nos resultados, concluiu-se que as sementes utilizadas pelos produtores no Estado, apresentam elevado nivel de infeccao por bactrias, e o tratamento por hidrotermoterapia nao foi eficiente no contrle das bacterias. 650 $aseeds 650 $aAreia 650 $aBactéria 650 $aControle Químico 650 $aDoença 650 $aEspécie 650 $aFeijão de Corda 650 $aSemente 650 $aVigna Unguiculata 650 $aXanthomonas Phaseoli 653 $aBrasil 653 $aDiseases 653 $aParaiba 653 $aPesudomona
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