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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
22/11/2001 |
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Data da última atualização: |
31/05/2007 |
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Autoria: |
SIMÕES-ARAÚJO-ARAUJO, J. L.; RODRIGUES, R. L.; GERHARDT, L. B. de A.; MONDEGO, J. M. C.; ALVES-FERREIRA, M.; RUMJANEK, N. G.; MARGIS-PINHEIRO, M. |
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Título: |
Identification of differentially expressed genes by cDNA-AFLP technique during heat stress in cowpea nodules. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
FEBS Letters, Netherlands, v. 515, p. 44-50, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Legume nodules formed by diazotrophic microorganisrns are active sites for biological nitrogen fixation (BNF). In tropical regions, a significant part of N supply for soybean, peanut and bean crops is derived from BNF , which is nevertheless often limited by high temperature stress. In contrast, cowpea nodules are very resistant to high temperatures. To understand the molecular bases of thermotolerance during BNF under heat stress, we have used cDNA-amplified fragment length polymorphism experiments to identify differentially expressed transcripts from cowpea nodules subjected to heat shock treatment. The expression profiles obtained showed approximately 600 bands, 55 up-regulated and nine corresponding to genes repressed by heat stress. Twenty transcript-derived fragments were isolated, cloned and sequenced. The Vigna unguiculata nodule and stress response transcripts present similarities to those that encode low molecular weight heat shock proteins, wound-induced proteins, disease resistance protein, and xylan endohydrolase isoenzyme, as well as different housekeeping genes. The differential expression of 15 genes was confirmed by using Northern blot or reverse Northern hybridization experiments. @ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B. V. AJI rights reserved.
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Palavras-Chave: |
Biological nitrogen fixation; BNF; Caupi; FBN; Fixação bioloógica de nitrogênio. |
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Thesagro: |
Vigna Unguiculata. |
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Thesaurus Nal: |
cowpeas. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02152naa a2200277 a 4500
001 1598731
005 2007-05-31
008 2002 bl --- 0-- u #d
100 1 $aSIMÕES-ARAÚJO-ARAUJO, J. L.
245 $aIdentification of differentially expressed genes by cDNA-AFLP technique during heat stress in cowpea nodules.
260 $c2002
520 $aLegume nodules formed by diazotrophic microorganisrns are active sites for biological nitrogen fixation (BNF). In tropical regions, a significant part of N supply for soybean, peanut and bean crops is derived from BNF , which is nevertheless often limited by high temperature stress. In contrast, cowpea nodules are very resistant to high temperatures. To understand the molecular bases of thermotolerance during BNF under heat stress, we have used cDNA-amplified fragment length polymorphism experiments to identify differentially expressed transcripts from cowpea nodules subjected to heat shock treatment. The expression profiles obtained showed approximately 600 bands, 55 up-regulated and nine corresponding to genes repressed by heat stress. Twenty transcript-derived fragments were isolated, cloned and sequenced. The Vigna unguiculata nodule and stress response transcripts present similarities to those that encode low molecular weight heat shock proteins, wound-induced proteins, disease resistance protein, and xylan endohydrolase isoenzyme, as well as different housekeeping genes. The differential expression of 15 genes was confirmed by using Northern blot or reverse Northern hybridization experiments. @ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B. V. AJI rights reserved.
650 $acowpeas
650 $aVigna Unguiculata
653 $aBiological nitrogen fixation
653 $aBNF
653 $aCaupi
653 $aFBN
653 $aFixação bioloógica de nitrogênio
700 1 $aRODRIGUES, R. L.
700 1 $aGERHARDT, L. B. de A.
700 1 $aMONDEGO, J. M. C.
700 1 $aALVES-FERREIRA, M.
700 1 $aRUMJANEK, N. G.
700 1 $aMARGIS-PINHEIRO, M.
773 $tFEBS Letters, Netherlands$gv. 515, p. 44-50, 2002.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
17/04/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
PICININ, L. C. A.; TOALDO, I. M.; HOFF, R. B.; SOUZA, F. N.; LEITE, M. O; FONSECA, L. M.; DINIZ, S. A.; SILVA, M. X.; CERQUEIRA, M. O. P.; BORDIGNON-LUZ, M. T. |
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Afiliação: |
LIDIA C. A. PICININ, UFSC; ISABELA M. TOALDO, UFSC; RODRIGO B. HOFF, LANAGRO/MAPA; FERNANDO N. SOUZA, UFMG; MÔNICA O. LEITE, UFMG; LEORGES M. FONSECA, UFMG; SORAIA A. DINIZ, UFMG; MARCOS X. SILVA, UFMG; MÔNICA M. O P. CERQUEIRA, UFMG; MARILDE T. BORDIGNON-LUIZ, UFSC. |
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Título: |
Survey of pyrethroid, macrocyclic lactone and antibacterial residues in bulk milk tank fraom Minas Gerais State, Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 2, p. 97-104, fev. 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
A survey of veterinary drug residues in bulk milk tank from Minas Gerais State, Brazil, was carried out through a broad scope analysis. Here, 132 raw milk samples were collected at 45 dairy farms in Minas Gerais from August 2009 to February 2010, and analyzed for 42 analytes, comprising pyrethroids, macrocyclic lactones and antibacterials, using liquid chromatography coupled with mass spectrometry in tandem mode and gas chromatography with electron capture detection. Within all milk samples, at least one veterinary drug residue was identified in 40 milk samples (30.30%) by confirmatory tests, whereas 16 samples (12.12%) showed the presence of at least two residues. With regard to the Brazilian maximum residue levels, 11 milk samples (8.33%) were non-compliant according to Brazilian Legislation. The veterinary drugs detected in the non-compliant milk samples include penicillin V (one sample), abamectin (one sample) and cypermethrin (nine samples). Furthermore, the antibacterial screening methods failed to identify most of the positive samples that were detected by confirmatory tests, leading to a large discrepancy between the screening and confirmatory antimicrobial tests. Thus, the present study indicated that the veterinary drugs residues still represents a great concern for the milk production chain. |
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Palavras-Chave: |
Antelmíntico; Leite cru; Medicamento veterinario. |
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Thesagro: |
Antibiótico; Pesticida. |
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Thesaurus NAL: |
Anthelmintics; Antibiotics; Pesticides; Raw milk; Veterinary drugs. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/159074/1/Survey-of-pyrethroid.pdf
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Marc: |
LEADER 02349naa a2200349 a 4500
001 2068563
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aPICININ, L. C. A.
245 $aSurvey of pyrethroid, macrocyclic lactone and antibacterial residues in bulk milk tank fraom Minas Gerais State, Brazil.$h[electronic resource]
260 $c2017
520 $aA survey of veterinary drug residues in bulk milk tank from Minas Gerais State, Brazil, was carried out through a broad scope analysis. Here, 132 raw milk samples were collected at 45 dairy farms in Minas Gerais from August 2009 to February 2010, and analyzed for 42 analytes, comprising pyrethroids, macrocyclic lactones and antibacterials, using liquid chromatography coupled with mass spectrometry in tandem mode and gas chromatography with electron capture detection. Within all milk samples, at least one veterinary drug residue was identified in 40 milk samples (30.30%) by confirmatory tests, whereas 16 samples (12.12%) showed the presence of at least two residues. With regard to the Brazilian maximum residue levels, 11 milk samples (8.33%) were non-compliant according to Brazilian Legislation. The veterinary drugs detected in the non-compliant milk samples include penicillin V (one sample), abamectin (one sample) and cypermethrin (nine samples). Furthermore, the antibacterial screening methods failed to identify most of the positive samples that were detected by confirmatory tests, leading to a large discrepancy between the screening and confirmatory antimicrobial tests. Thus, the present study indicated that the veterinary drugs residues still represents a great concern for the milk production chain.
650 $aAnthelmintics
650 $aAntibiotics
650 $aPesticides
650 $aRaw milk
650 $aVeterinary drugs
650 $aAntibiótico
650 $aPesticida
653 $aAntelmíntico
653 $aLeite cru
653 $aMedicamento veterinario
700 1 $aTOALDO, I. M.
700 1 $aHOFF, R. B.
700 1 $aSOUZA, F. N.
700 1 $aLEITE, M. O
700 1 $aFONSECA, L. M.
700 1 $aDINIZ, S. A.
700 1 $aSILVA, M. X.
700 1 $aCERQUEIRA, M. O. P.
700 1 $aBORDIGNON-LUZ, M. T.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 2, p. 97-104, fev. 2017.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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