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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
19/04/2023 |
Data da última atualização: |
19/04/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RIEGER, J. S. G.; MANTOVANI, C.; SUNIGA, P. A. P.; PINTO, I. B.; SOUSA, G. A. A.; GAVA, D.; CANTAO, M. E.; SANTOS, L. R.; ARAÚJO, F. R.; ZANELLA, J. R. C. |
Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
Título: |
Standardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 22, n. 1, ed. gmr19118, 2023. |
DOI: |
http://dx.doi.org/10.4238/gmr19118 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. MenosAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here wa... Mostrar Tudo |
Thesagro: |
Diagnostico. |
Thesaurus Nal: |
Senecavirus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153239/1/final10100.pdf
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Marc: |
LEADER 02545naa a2200265 a 4500 001 2153239 005 2023-04-19 008 2023 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.4238/gmr19118$2DOI 100 1 $aRIEGER, J. S. G. 245 $aStandardization of ELISA with senecavirus A recombinant VP2 protein and its use in swine herds in Brazil.$h[electronic resource] 260 $c2023 520 $aAbstract: Senecavirus A (SVA) is a nonenveloped, single-stranded RNA virusThe icosahedral viral particle is composed of four structural proteins: VP1, VP2, VP3 and VP4, among which VP2 is strongly involved in the antibody immune response. The virus causes vesicles on the snout and feet in pigs, which are clinically indistinguishable from other vesicular diseases such as foot-and-mouth disease. Outbreaks of SVA have been reported worldwide since 2014; however, its prevalence in Brazil remains unknown. In this study, the VP2 structural protein was produced and purified from E. coli, and recombinant VP2 (rVP2), based on the most recent Brazilian strain, was used to develop an indirect ELISA to identify antibodies against SVA in Brazilian swine herds. Sensitivity and specificity values of the rVP2 ELISA were determined using receiver operating characteristic analysis performed on 43 SVA positive and 219 negative serum samples. In addition, serum samples from pigs immunized with eight distinct Brazilian SVA inactivated strains were tested with the rVP2 ELISA. For the specificity of the assay, 17 serum samples from vesicular stomatitis virus (VSV) from naturally infected pigs were tested. The rVP2 ELISA was found to have 100% specificity and 74.4% sensitivity. The performance of the assay using samples collected during the SVA outbreak, had a sensitivity of 100%, and with those collected nine months after the outbreak it had a sensitivity of 73.4%. The rVP2 ELISA developed here was able to detect specific SVA antibodies in acute disease and recovered pigs, and no cross-reactivity with VSV was observed. This assay has potential as a useful tool for monitoring SVA infection and could help to improve disease diagnosis. 650 $aSenecavirus 650 $aDiagnostico 700 1 $aMANTOVANI, C. 700 1 $aSUNIGA, P. A. P. 700 1 $aPINTO, I. B. 700 1 $aSOUSA, G. A. A. 700 1 $aGAVA, D. 700 1 $aCANTAO, M. E. 700 1 $aSANTOS, L. R. 700 1 $aARAÚJO, F. R. 700 1 $aZANELLA, J. R. C. 773 $tGenetics and Molecular Research$gv. 22, n. 1, ed. gmr19118, 2023.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Milho e Sorgo. |
Data corrente: |
10/02/2009 |
Data da última atualização: |
31/10/2023 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
ALVARENGA, R. C.; COBUCCI, T.; KLUTHCOUSKI, J.; WRUCK, F. J.; CRUZ, J. C.; GONTIJO NETO, M. M. |
Afiliação: |
RAMON COSTA ALVARENGA, CNPMS; TARCISIO COBUCCI, CNPAF; JOAO KLUTHCOUSKI, CNPAF; FLAVIO JESUS WRUCK, CNPAF; JOSE CARLOS CRUZ, CNPMS; MIGUEL MARQUES GONTIJO NETO, CNPMS. |
Título: |
A cultura do milho na integração lavoura-pecuária. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CRUZ, J. C.; KARAM, D.; MONTEIRO, M. A. R.; MAGALHAES, P. C. (Ed.). A cultura do milho. Sete Lagoas: Embrapa Milho e Sorgo, 2008. cap. 16, p. 491-517. |
Idioma: |
Português |
Conteúdo: |
Introdução. Vantagens da integração lavoura-pecuária. Milho consorciado com forrageiras. Sistema Barreirão. Sistema Santa Fé. Fisiologia das espécies em consórcio. Manejos de herbicidas e efeitos no milho e na produção de forragem. Arranjos espaciais da forrageira e efeito no milho e na produção de forragem. Épocas de introdução das forrageiras e efeitos no milho e na produção de forragem. Colheita do milho. |
Thesagro: |
Consorciação de cultura; Milho; Sistema de cultivo; Zea Mays. |
Thesaurus NAL: |
Cropping systems; Intercropping. |
Categoria do assunto: |
A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/55982/1/Cultura-milho.pdf
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Marc: |
LEADER 01226naa a2200253 a 4500 001 1491714 005 2023-10-31 008 2008 bl uuuu u00u1 u #d 100 1 $aALVARENGA, R. C. 245 $aA cultura do milho na integração lavoura-pecuária.$h[electronic resource] 260 $c2008 520 $aIntrodução. Vantagens da integração lavoura-pecuária. Milho consorciado com forrageiras. Sistema Barreirão. Sistema Santa Fé. Fisiologia das espécies em consórcio. Manejos de herbicidas e efeitos no milho e na produção de forragem. Arranjos espaciais da forrageira e efeito no milho e na produção de forragem. Épocas de introdução das forrageiras e efeitos no milho e na produção de forragem. Colheita do milho. 650 $aCropping systems 650 $aIntercropping 650 $aConsorciação de cultura 650 $aMilho 650 $aSistema de cultivo 650 $aZea Mays 700 1 $aCOBUCCI, T. 700 1 $aKLUTHCOUSKI, J. 700 1 $aWRUCK, F. J. 700 1 $aCRUZ, J. C. 700 1 $aGONTIJO NETO, M. M. 773 $tIn: CRUZ, J. C.; KARAM, D.; MONTEIRO, M. A. R.; MAGALHAES, P. C. (Ed.). A cultura do milho. Sete Lagoas: Embrapa Milho e Sorgo, 2008. cap. 16, p. 491-517.
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Embrapa Milho e Sorgo (CNPMS) |
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