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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
22/02/2011 |
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Data da última atualização: |
10/03/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMIRO, D. A.; ESCOUTE, J.; PETITOT, A.-S.; NICOLE, M.; MALUF, M. P.; FERNANDEZ, D. |
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Afiliação: |
INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; CIRAD; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT; MIRIAN PEREZ MALUF, SAPC; INSTITUTE DE RECHERCHE POUR LE DEVELOPPEMENT. |
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Título: |
Biphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defense responses in resistant and susceptible coffee cultivars. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In.: PLANT PATHOLOGY, v. 58, p. 944-955, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secreted into, the subsidiary and adjacent cells of the stomata. Additionally, the present study provides new insights into the colonization process of the coffee rust fungus. MenosThe objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secrete... Mostrar Tudo |
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Palavras-Chave: |
Pathogenesis-related genes; Quantitative real-time PCR. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Reactive oxygen species. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29268/1/Biphasic-haustorial.pdf
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Marc: |
LEADER 02423naa a2200229 a 4500
001 1880368
005 2011-03-10
008 2009 bl uuuu u00u1 u #d
100 1 $aRAMIRO, D. A.
245 $aBiphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defense responses in resistant and susceptible coffee cultivars.$h[electronic resource]
260 $c2009
520 $aThe objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secreted into, the subsidiary and adjacent cells of the stomata. Additionally, the present study provides new insights into the colonization process of the coffee rust fungus.
650 $aReactive oxygen species
650 $aCoffea Arábica
653 $aPathogenesis-related genes
653 $aQuantitative real-time PCR
700 1 $aESCOUTE, J.
700 1 $aPETITOT, A.-S.
700 1 $aNICOLE, M.
700 1 $aMALUF, M. P.
700 1 $aFERNANDEZ, D.
773 $tIn.: PLANT PATHOLOGY$gv. 58, p. 944-955, 2009.
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Registro original: |
Embrapa Café (CNPCa) |
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Biblioteca |
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| Registros recuperados : 69 | |
| 23. |  | GALLEP, C. M.; AMARAL, E. M. do; ALONCO, G. C.; MALUF, M. P.; PADILHA, L. Germination vigour versus delayed luminescence of coffee seeds: preliminary series. In: INTERNATIONAL CONFERENCE ON PHOTONICS, OPTICS AND LASER TECHNOLOGY, 2., 2014, Lisboa. Photoptics 2014. Lisboa, 2014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 24. | | MALUF, M. P.; SILVESTRINI, M.; RUGGIERO, L. M. de C.; GUERREIRO FILHO, O.; COLOMBO, C. A. Genetic diversity of cultivated Coffea arabica inbred lines assessed by RAPD, AFLP and SSR marker systems. Scientia Agricola, v. 62, n. 4, p. 366-373, 2005. Título em português: Caracterização da diversidade genética de linhagens comerciais de Coffea arabica através de marcadores moleculares do tipo RAPD, AFLP e SSR.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Café. |
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| 28. | | ORTEGA, H. M.; PADILHA, L.; GUERREIRO FILHO, O.; GONÇALVES, W.; MALUF, M. P.; BRAGINI, M. T. Microssatellite for fingerprinting Coffea arabica clones. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 202| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 34. | | PEZZOPANE, C. de G.; FAVARIN, J. L.; MALUF, M. P.; PEZZOPANE, J. R. M.; GUERREIRO FILHO, O. Atributos fenológicos e agronômicos em cultivares de cafeeiro arábica. In.: CIÊNCIA RURAL, Santa Maria, v.39, n.3, p.711-717, mai-jun. 2009. Título em inglês: Phenological and agronimic attributes in arabica cultivars of coffee tree.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Café. |
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| 35. | | SEVERINO, F. E.; BRANDALISE, M.; COSTA, C. S.; WILCKEN, S. R. S.; MALUF, M. P.; GONÇALVES, W.; MAIA, I. G. CaPrx, a Coffea arabica gene encoding a putative class III peroxidase induced by root-knot nematode infection. Plant Science, Limerick, v. 191-192, p. 35-42, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| 36. | | MARTINATI, J. C.; CARDOSO, D. C.; GUERREIRO-FILHO, O.; VIDAL, R. O.; CARAZZOLLE, M. F.; MALUF, M. P. Caracterização molecular da interação cafeeiro/bicho-mineiro. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 37. | | GASPARI-PEZZOPANE, C. de; FAVARIN, J. L.; MALUF, M. P.; PEZZOPANE, J. R. M.; MISTRO, J. C.; SALGADO, P. R. Metodologia para avaliação do comportamento fenológico de cultivares de Coffea arabica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 38. | | GUIMARÃES, P. de S.; SCHENK, J. C. M.; GIACHETTO, P. F.; PADILHA, L.; SILVAROLLA, M. B.; MALUF, M. P. Validação de retrotransposons ativos em café-efeitos de estresse abiótico e baixa cafeína na expressão gênica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória. Pesquisa, Inovação e Sustentabilidade dos Cafés do Brasil. Anais... Brasília, DF: Embrapa Café, 2019. Título em inglês: Validation of active retrotransposons in coffee-effects of abiotic stress and low caffeine content on gene expression.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 39. | | GUIMARÃES, P. de S.; SCHENK, J. C. M.; GIACHETTO, P. F.; PADILHA, L.; SILVAROLLA, M. B.; MALUF, M. P. Validação de retrotransposons ativos em café-efeitos de estresse abiótico e baixa cafeína na expressão gênica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019. 4 p. Título em inglês: Validation of active retrotransposons in coffee-effects of abiotic stress and low caffeine content on gene expression.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 40. | | MARTINATI, J. de C.; MOLINA, J. V. D.; BONTURI, N.; MARTARELLO, F. H. H.; GUERREIRO FILHO, O.; MALUF, M. P. Expressão de genes relacionados ao metabolismo de nitrogênio, fósforo de potássio em cafeeiros submetidos ao estresse biótico. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Inovação científica, competitividade e mudanças climáticas: anais... Vitória: Consórcio Pesquisa Café, 2009.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 69 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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