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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
19/07/2006 |
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Data da última atualização: |
13/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
WAGNER, R.; MITCHEL, D. A.; SASSAKI, G. L.; AMAZONAS, M. A. L. de A. |
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Afiliação: |
Pesquisador da Embrapa Florestas: Maria Ângela L. de A. Amazonas. |
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Título: |
Links between morphology and physiology of Ganoderma lucidum in submerged culture for the production of exopolysaccharide. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Journal of Biotechnology, v. 114, p. 153-164, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Ganoderma lucidum was grown in submerged culture in shake flasks on a medium containing peptone, yeast extract and glucose. In pre-cultures, inoculated from an agar-grown culture, morphological and metabolic events were linked: the pellets originally produced protuberances when glucose was present in the medium, although glucose was not consumed. The protuberances were then liberated into the medium as second-generation pellets, at which time glucose consumption began and the rate of exopolysaccharide (EPS) production increased. The synchrony between events was repeated in cultures fed with either glucose or peptone and yeast extract. In main cultures, inoculated from a 16-day-old pre-culture, the biomass concentration increased linearly, while glucose consumption and EPS production were initially slow but then accelerated. Protuberances were produced and liberated similarly to the pre-culture, but there was less synchrony amongst the pellets. When glucose was added to such a culture on day 10, an EPS concentration of 5.7 g L?1 was achieved on day 13, this being the highest reliable EPS concentration yet reported for submerged culture of G. lucidum.We conclude that a greater understanding of the morphological and physiological events during the culture of G. lucidum will allow the proposal of culture strategies to improve EPS production. © 2004 Elsevier B.V. All rights reserved. |
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Palavras-Chave: |
Exopolysaccharide; Submerged culture. |
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Thesaurus NAL: |
Ganoderma lucidum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02024naa a2200193 a 4500
001 1313716
005 2015-02-13
008 2004 bl uuuu u00u1 u #d
100 1 $aWAGNER, R.
245 $aLinks between morphology and physiology of Ganoderma lucidum in submerged culture for the production of exopolysaccharide.$h[electronic resource]
260 $c2004
520 $aGanoderma lucidum was grown in submerged culture in shake flasks on a medium containing peptone, yeast extract and glucose. In pre-cultures, inoculated from an agar-grown culture, morphological and metabolic events were linked: the pellets originally produced protuberances when glucose was present in the medium, although glucose was not consumed. The protuberances were then liberated into the medium as second-generation pellets, at which time glucose consumption began and the rate of exopolysaccharide (EPS) production increased. The synchrony between events was repeated in cultures fed with either glucose or peptone and yeast extract. In main cultures, inoculated from a 16-day-old pre-culture, the biomass concentration increased linearly, while glucose consumption and EPS production were initially slow but then accelerated. Protuberances were produced and liberated similarly to the pre-culture, but there was less synchrony amongst the pellets. When glucose was added to such a culture on day 10, an EPS concentration of 5.7 g L?1 was achieved on day 13, this being the highest reliable EPS concentration yet reported for submerged culture of G. lucidum.We conclude that a greater understanding of the morphological and physiological events during the culture of G. lucidum will allow the proposal of culture strategies to improve EPS production. © 2004 Elsevier B.V. All rights reserved.
650 $aGanoderma lucidum
653 $aExopolysaccharide
653 $aSubmerged culture
700 1 $aMITCHEL, D. A.
700 1 $aSASSAKI, G. L.
700 1 $aAMAZONAS, M. A. L. de A.
773 $tJournal of Biotechnology$gv. 114, p. 153-164, 2004.
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