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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
13/10/1999 |
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Data da última atualização: |
13/10/1999 |
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Autoria: |
MAJER, D.; MITHEN, R.; LEWIS, B. G.; VOS, P.; OLIVER, R. P. |
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Afiliação: |
Department of Brassica and Oilseed Research, John Innes Centre, ColneyLane, Norwich NR4 7UH, U.K. |
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Título: |
The use of AFLP fingerprinting for the detection of genetic variation in fungi |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Mycological Research, v.100, n.9, p.1107-1111, 1996 |
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Idioma: |
Inglês |
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Conteúdo: |
A new PCR-based technique for the detection of inter-and intraspecific genetic variation has been tested on isolates of the fungal phytopathogens Cladosporium fulvum and Pyrenopeziza brassicae. The method is based on the selective PCR amplification of restriction fragments from digests of genomic DNA. We show that the technique is very efficient at detecting polymorphisms, even in species where very llittle variationcould previously be found by RFLP analysis. 21 primer combinations were used on four isolates of P. brassicae, detecting a total of 162 polymorphisms (mean = 4.I polymorphisms per primer combination per pair of isolates). |
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Categoria do assunto: |
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Marc: |
LEADER 01100naa a2200169 a 4500
001 1648336
005 1999-10-13
008 1996 bl --- 0-- u #d
100 1 $aMAJER, D.
245 $aThe use of AFLP fingerprinting for the detection of genetic variation in fungi
260 $c1996
520 $aA new PCR-based technique for the detection of inter-and intraspecific genetic variation has been tested on isolates of the fungal phytopathogens Cladosporium fulvum and Pyrenopeziza brassicae. The method is based on the selective PCR amplification of restriction fragments from digests of genomic DNA. We show that the technique is very efficient at detecting polymorphisms, even in species where very llittle variationcould previously be found by RFLP analysis. 21 primer combinations were used on four isolates of P. brassicae, detecting a total of 162 polymorphisms (mean = 4.I polymorphisms per primer combination per pair of isolates).
700 1 $aMITHEN, R.
700 1 $aLEWIS, B. G.
700 1 $aVOS, P.
700 1 $aOLIVER, R. P.
773 $tMycological Research$gv.100, n.9, p.1107-1111, 1996
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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