Data/hora: 09/06/2024 19:37:13

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 13/10/1999
Data da última atualização: | 13/10/1999
Autoria: | MAJER, D.; MITHEN, R.; LEWIS, B. G.; VOS, P.; OLIVER, R. P.
Afiliação: | Department of Brassica and Oilseed Research, John Innes Centre, ColneyLane, Norwich NR4 7UH, U.K.
Título: | The use of AFLP fingerprinting for the detection of genetic variation in fungi
Ano de publicação: | 1996
Fonte/Imprenta: | Mycological Research, v.100, n.9, p.1107-1111, 1996
Idioma: | Inglês
Conteúdo: | A new PCR-based technique for the detection of inter-and intraspecific genetic variation has been tested on isolates of the fungal phytopathogens Cladosporium fulvum and Pyrenopeziza brassicae. The method is based on the selective PCR amplification of restriction fragments from digests of genomic DNA. We show that the technique is very efficient at detecting polymorphisms, even in species where very llittle variationcould previously be found by RFLP analysis. 21 primer combinations were used on four isolates of P. brassicae, detecting a total of 162 polymorphisms (mean = 4.I polymorphisms per primer combination per pair of isolates).
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (17368-1) - ADD - SP - -- - S11533 -  - S11533 - DIS