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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
14/05/1997 |
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Data da última atualização: |
01/06/2005 |
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Autoria: |
MAGALHAES, B. P. |
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Afiliação: |
EMBRAPA-CENARGEN. |
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Título: |
Controle biologico de gafanhotos. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
In: SIMPOSIO DE CONTROLE BIOLOGICO, 4., 1994, Gramado, RS. Anais: Conferencias e mesas redondas. Pelotas, RS: EMBRAPA/CPACT, 1994. |
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Páginas: |
p.23-27. |
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Série: |
(EMBRAPA-CPACT. Documentos, 6). |
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Idioma: |
Português |
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Palavras-Chave: |
Grasshopper. |
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Thesagro: |
Controle Biológico; Gafanhoto. |
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Thesaurus Nal: |
biological control. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00549nam a2200169 a 4500
001 1170959
005 2005-06-01
008 1994 bl uuuu u01u1 u #d
100 1 $aMAGALHAES, B. P.
245 $aControle biologico de gafanhotos.
260 $aIn: SIMPOSIO DE CONTROLE BIOLOGICO, 4., 1994, Gramado, RS. Anais: Conferencias e mesas redondas. Pelotas, RS: EMBRAPA/CPACT$c1994
300 $ap.23-27.
490 $a(EMBRAPA-CPACT. Documentos, 6).
650 $abiological control
650 $aControle Biológico
650 $aGafanhoto
653 $aGrasshopper
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
07/06/2004 |
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Data da última atualização: |
05/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIEIRA, E. C.; NOGUEIRA, A. R. de A. |
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Afiliação: |
EDIVAN CARVALHO VIEIRA, UFSCAR; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
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Título: |
Orthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, v.52, p.1800-1803, 2004. |
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DOI: |
https://doi.org/10.1021/jf034467f |
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Idioma: |
Inglês |
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Conteúdo: |
A flow injection spectrophotometric procedure with enzymatic hydrolysis was developed for determination of orthophosphate, phytate and total phosphorus in cereal samples. Phosphorus species were extracted from cereals with 0.05 mol L-1 potassium hydrogen phthalate buffer solution at pH 5.7. Orthophosphate was directly determined in the extracts by molybdenum blue spectrophotometric method. The phytate was hydrolyzed by the enzyme phytase coupled to a solid phase packed into an enzymatic reactor, and the resulting hydrolyzed orthophosphate was also determined by spectrophotometry at 650 nm. After optimization for phosphorus species extraction and enzymatic hydrolysis, a linear calibration graph was obtained up to 196 × 10-6 mol L-1 orthophosphate (P conc) -2.67 + 0.52x, r ) 0.9998). Measurements are characterized by relative standard deviation of 1.6% for a standard of 72 × 10-6 mol L-1 orthophosphate and no baseline drift was observed during 4 h operation periods. It provides 72 measurements per hour, with 2.4 × 10-6 mol L-1 and 7.9 × 10-6 mol L-1 as detection and quantification limits, respectively. |
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Palavras-Chave: |
Acid; Enzymatic hysrolysis; Immobilization; Phytase; Phytic; Spectophometry. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/88222/1/PROCI-2004.00041.pdf
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Marc: |
LEADER 01793naa a2200217 a 4500
001 1046840
005 2022-05-05
008 2004 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1021/jf034467f$2DOI
100 1 $aVIEIRA, E. C.
245 $aOrthophosphate, phytate, and total phosohorus determination in cerals by flow injection analysis.$h[electronic resource]
260 $c2004
520 $aA flow injection spectrophotometric procedure with enzymatic hydrolysis was developed for determination of orthophosphate, phytate and total phosphorus in cereal samples. Phosphorus species were extracted from cereals with 0.05 mol L-1 potassium hydrogen phthalate buffer solution at pH 5.7. Orthophosphate was directly determined in the extracts by molybdenum blue spectrophotometric method. The phytate was hydrolyzed by the enzyme phytase coupled to a solid phase packed into an enzymatic reactor, and the resulting hydrolyzed orthophosphate was also determined by spectrophotometry at 650 nm. After optimization for phosphorus species extraction and enzymatic hydrolysis, a linear calibration graph was obtained up to 196 × 10-6 mol L-1 orthophosphate (P conc) -2.67 + 0.52x, r ) 0.9998). Measurements are characterized by relative standard deviation of 1.6% for a standard of 72 × 10-6 mol L-1 orthophosphate and no baseline drift was observed during 4 h operation periods. It provides 72 measurements per hour, with 2.4 × 10-6 mol L-1 and 7.9 × 10-6 mol L-1 as detection and quantification limits, respectively.
653 $aAcid
653 $aEnzymatic hysrolysis
653 $aImmobilization
653 $aPhytase
653 $aPhytic
653 $aSpectophometry
700 1 $aNOGUEIRA, A. R. de A.
773 $tJournal of Agricultural and Food Chemistry$gv.52, p.1800-1803, 2004.
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Embrapa Pecuária Sudeste (CPPSE) |
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