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Registro Completo |
Biblioteca(s): |
Embrapa Agropecuária Oeste. |
Data corrente: |
19/06/1995 |
Data da última atualização: |
19/06/1995 |
Autoria: |
MAGALHAES, A. R.; REBOUCAS, O. E. |
Título: |
Conclusions. |
Ano de publicação: |
1987 |
Fonte/Imprenta: |
In: MAGALHAES, A.R. et al. The effects of climatic variations on agriculture in Northeast Brazil. Laxenburg: IIASA, 1987. |
Páginas: |
p.97-103 |
Idioma: |
Inglês |
Categoria do assunto: |
-- |
Marc: |
LEADER 00378nam a2200121 a 4500 001 1238264 005 1995-06-19 008 1987 bl uuuu 00u1 u #d 100 1 $aMAGALHAES, A. R. 245 $aConclusions. 260 $aIn: MAGALHAES, A.R. et al. The effects of climatic variations on agriculture in Northeast Brazil. Laxenburg: IIASA$c1987 300 $ap.97-103 700 1 $aREBOUCAS, O. E.
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Embrapa Agropecuária Oeste (CPAO) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
13/06/2016 |
Data da última atualização: |
19/06/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ARDISSON-ARAÚJO, D. M. P.; LIMA, R. N.; MELO, F. L.; CLEM, R. J.; HUANG, N.; BÁO, S. N.; SOSA-GÓMEZ, D. R.; RIBEIRO, B. M. |
Afiliação: |
DANIEL M. P. ARDISSON-ARAÚJO, UNB; RAYANE NUNES LIMA, UNB; FERNANDO L. MELO, UNB; ROLLIE J. CLEM, KANSAS STATE UNIVERSITY; NING HUANG, Kansas State University; SÔNIA NAIR BÁO, UNB; DANIEL RICARDO SOSA GOMEZ, CNPSO; BERGMANN M. RIBEIRO, UNB. |
Título: |
Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Scientific Reports, v. 6, n. 24612, jun. 2016. |
ISSN: |
2045-2322 |
DOI: |
10.1038/srep24612 |
Idioma: |
Inglês |
Conteúdo: |
The genome of a novel group II alphabaculovirus, Perigonia lusca single nucleopolyhedrovirus (PeluSNPV), was sequenced and shown to contain 132,831bp with 145 putative ORFs (open reading frames) of at least 50 amino acids. An interesting feature of this novel genome was the presence of a putative nucleotide metabolism enzyme-encoding gene (pelu112). The pelu112 gene was predicted to encode a fusion of thymidylate kinase (tmk) and dUTP diphosphatase (dut). Phylogenetic analysis indicated that baculoviruses have independently acquired tmk and dut several times during their evolution. Two homologs of the tmk-dut fusion gene were separately introduced into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome, which lacks tmk and dut. The recombinant baculoviruses produced viral DNA, virus progeny, and some viral proteins earlier during in vitro infection and the yields of viral occlusion bodies were increased 2.5-fold when compared to the parental virus. Interestingly, both enzymes appear to retain their active sites, based on separate modeling using previously solved crystal structures. We suggest that the retention of these tmk-dut fusion genes by certain baculoviruses could be related to accelerating virus replication and to protecting the virus genome from deleterious mutation. |
Thesagro: |
Baculovirus; Genoma; Virus. |
Thesaurus NAL: |
Baculoviridae; Genome assembly. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/144282/1/Genome-sequence-of-Perigonia-lusca-2016.pdf
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Marc: |
LEADER 02182naa a2200289 a 4500 001 2046809 005 2017-06-19 008 2016 bl uuuu u00u1 u #d 022 $a2045-2322 024 7 $a10.1038/srep24612$2DOI 100 1 $aARDISSON-ARAÚJO, D. M. P. 245 $aGenome sequence of Perigonia lusca single nucleopolyhedrovirus$binsights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae.$h[electronic resource] 260 $c2016 520 $aThe genome of a novel group II alphabaculovirus, Perigonia lusca single nucleopolyhedrovirus (PeluSNPV), was sequenced and shown to contain 132,831bp with 145 putative ORFs (open reading frames) of at least 50 amino acids. An interesting feature of this novel genome was the presence of a putative nucleotide metabolism enzyme-encoding gene (pelu112). The pelu112 gene was predicted to encode a fusion of thymidylate kinase (tmk) and dUTP diphosphatase (dut). Phylogenetic analysis indicated that baculoviruses have independently acquired tmk and dut several times during their evolution. Two homologs of the tmk-dut fusion gene were separately introduced into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome, which lacks tmk and dut. The recombinant baculoviruses produced viral DNA, virus progeny, and some viral proteins earlier during in vitro infection and the yields of viral occlusion bodies were increased 2.5-fold when compared to the parental virus. Interestingly, both enzymes appear to retain their active sites, based on separate modeling using previously solved crystal structures. We suggest that the retention of these tmk-dut fusion genes by certain baculoviruses could be related to accelerating virus replication and to protecting the virus genome from deleterious mutation. 650 $aBaculoviridae 650 $aGenome assembly 650 $aBaculovirus 650 $aGenoma 650 $aVirus 700 1 $aLIMA, R. N. 700 1 $aMELO, F. L. 700 1 $aCLEM, R. J. 700 1 $aHUANG, N. 700 1 $aBÁO, S. N. 700 1 $aSOSA-GÓMEZ, D. R. 700 1 $aRIBEIRO, B. M. 773 $tScientific Reports$gv. 6, n. 24612, jun. 2016.
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