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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Trigo. |
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Data corrente: |
04/09/2014 |
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Data da última atualização: |
01/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ZHOU, G.; PEREIRA, J. F.; DELHAIZE, E.; ZHOU, M.; MAGALHAES, J. V.; RYAN, P. R. |
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Afiliação: |
UNIVERSITY OF TASMANIA; JORGE FERNANDO PEREIRA, CNPT; CSIRO PLANT INDUSTRY; UNIVERSITY OF TASMANIA; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CSIRO PLANT INDUSTRY. |
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Título: |
Enhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Experimental Botany, London, v. 65, n. 9, p. 2381-2390, 2014. |
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DOI: |
10.1093/jxb/eru121 |
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Idioma: |
Inglês |
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Conteúdo: |
Malate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species. MenosMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tole... Mostrar Tudo |
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Palavras-Chave: |
Citrato; Tolerância ao alumínio; Transgênico. |
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Thesagro: |
Cevada; Solo Ácido. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117026/1/2014-Journal-of-Experimental-Botany-v65n9p2381.pdf
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Marc: |
LEADER 02283naa a2200253 a 4500
001 2007369
005 2015-02-01
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1093/jxb/eru121$2DOI
100 1 $aZHOU, G.
245 $aEnhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3.$h[electronic resource]
260 $c2014
520 $aMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species.
650 $aCevada
650 $aSolo Ácido
653 $aCitrato
653 $aTolerância ao alumínio
653 $aTransgênico
700 1 $aPEREIRA, J. F.
700 1 $aDELHAIZE, E.
700 1 $aZHOU, M.
700 1 $aMAGALHAES, J. V.
700 1 $aRYAN, P. R.
773 $tJournal of Experimental Botany, London$gv. 65, n. 9, p. 2381-2390, 2014.
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Registro original: |
Embrapa Trigo (CNPT) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
15/12/2010 |
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Data da última atualização: |
15/12/2010 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MARQUES, R. F.; PADOVAN, M. P.; MOTTA, I. de S.; MOITINHO, M. R.; FERNANDES, S. S. |
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Afiliação: |
RODOLFO FREIRE MARQUES, UFGD; MILTON PARRON PADOVAN, CPAO; IVO DE SA MOTTA, CPAO; MARA REGINA MOITINHO, UNIGRAN; SHALINE SÉFARA FERNANDES, UFGD. |
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Título: |
Supressão de plantas espontâneas com adubação verde de primavera/verão em agroecossistema sob bases ecológicas, em Dourados, MS. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: SEMINÁRIO DE AGROECOLOGIA DE MATO GROSSO DO SUL, 3.; ENCONTRO DE PRODUTORES AGROECOLÓGICOS DE MS, 2., 2010, Corumbá. Construindo um futuro sustentável e solidário: anais. Dourados: Embrapa Agropecuária Oeste; Corumbá: Embrapa Pantanal; Campo Grande: Embrapa Gado de Corte, 2010. Editado por: Milton Parron Padovan, Ivo de Sá Motta, Hamilton Kikuti, Aurélio V. Borsato, Rogério Ferreira da Silva, Adalgiza Inês Campolim, Alzira Salet Menegat, Alberto Faiden. 1 CD-ROM. |
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ISBN: |
978-85-7540-026-5 |
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Idioma: |
Português |
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Palavras-Chave: |
Ecossystem; Green manure. |
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Thesagro: |
Adubação Verde. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/24215/1/Agroecologia22.pdf
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Marc: |
LEADER 01060nam a2200193 a 4500
001 1870033
005 2010-12-15
008 2010 bl uuuu u00u1 u #d
020 $a978-85-7540-026-5
100 1 $aMARQUES, R. F.
245 $aSupressão de plantas espontâneas com adubação verde de primavera/verão em agroecossistema sob bases ecológicas, em Dourados, MS.
260 $aIn: SEMINÁRIO DE AGROECOLOGIA DE MATO GROSSO DO SUL, 3.; ENCONTRO DE PRODUTORES AGROECOLÓGICOS DE MS, 2., 2010, Corumbá. Construindo um futuro sustentável e solidário: anais. Dourados: Embrapa Agropecuária Oeste; Corumbá: Embrapa Pantanal; Campo Grande: Embrapa Gado de Corte, 2010. Editado por: Milton Parron Padovan, Ivo de Sá Motta, Hamilton Kikuti, Aurélio V. Borsato, Rogério Ferreira da Silva, Adalgiza Inês Campolim, Alzira Salet Menegat, Alberto Faiden. 1 CD-ROM.$c2010
650 $aAdubação Verde
653 $aEcossystem
653 $aGreen manure
700 1 $aPADOVAN, M. P.
700 1 $aMOTTA, I. de S.
700 1 $aMOITINHO, M. R.
700 1 $aFERNANDES, S. S.
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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