Data/hora: 07/06/2024 13:25:10

Biblioteca(s): 	Embrapa Agroenergia.	
Data corrente: 	02/05/2024	
Data da última atualização: 	10/05/2024	
Autoria: 	LIMA, A. K. O.; SILVEIRA, A. P.; SILVA, R. C.; MACHADO, Y. A. A.; ARAÚJO, A. R. de; ARAUJO, S. S. de M.; VIEIRA, I. R. S.; ARAÚJO, J. L.; SANTOS, L. C. dos; RODRIGUES, K. A. da F.; SILVA, S. W. da; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; GARCIA, M. P.	
Afiliação: 	ALAN KELBIS OLIVEIRA LIMA; ARIANE PANDOLFO SILVEIRA; RENATA CARVALHO SILVA; YASMIN ALVES AIRES MACHADO; ALYNE RODRIGUES DE ARAÚJO; SANSARA SANNY DE MENDONÇA ARAUJO; ITALO RENNAN SOUSA VIEIRA; JOABE LIMA ARAÚJO; LUCAS CARVALHO DOS SANTOS; KLINGER ANTÔNIO DA FRANÇA RODRIGUES; SEBASTIÃO WILLIAM DA SILVA; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; CLENILSON MARTINS RODRIGUES, CNPAE; MÔNICA PEREIRA GARCIA.	
Título: 	Phytosynthesis of silver nanoparticles using guarana (Paullinia cupana Kunth) leaf extract employing different routes: characterization and investigation of in vitro bioactivities	
Ano de publicação: 	2024	
Fonte/Imprenta: 	Biomass Conversion and Biorefinery, v. 14, n. 10, 2024.	
DOI: 	https://doi.org/10.1007/s13399-023-05250-1	
Idioma: 	Inglês	
Conteúdo: 	The optimization techniques are fundamental to guarantee the stability of any preparation process and the quality of the nanostructures synthesized under ideal circumstances. In this sense, this study evaluates the potential of guarana leaf extract (Paullinia cupana Kunth—Sapindaceae) for the green synthesis of silver nanoparticles (AgNPs) using routes that employ different equipment/energy sources (autoclave, LED, microwave, sunlight, ultrasound, and water bath). The aqueous extract was characterized by high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLCHRMS/ MS). The AgNPs were analyzed by UV/Vis spectroscopy, dynamic light scattering (DLS), zeta potential (ZP), Fourier transform infrared (FTIR), transmission electron microscopy (TEM) and their antibacterial, leishmanicidal and cytotoxic effects were evaluated in vitro. The results show the presence of phenolic acids, alkaloids, and flavonoids in the leaf extract, which was supported by the identification of their functional groups in the FTIR spectra. The AgNPs showed maximum absorption between 420 and 440 nm (UV/Vis), with a diameter below 100 nm (DLS) and 60 nm (TEM), spherical morphology and a surface charge above − 30 mV (ZP). The antibacterial test showed pronounced inhibition in the growth of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus bacteria. The leishmanicidal activity of AgNPs on Leishmania (Leishmania) amazonensis was more pronounced than that of the drug miltefosine while low cytotoxicity was reported on RAW 264.7 macrophages. With this, this study reports the possibility of using alternative routes for the sustainable synthesis of AgNPs from Paullinia cupana extract with considerable applications against pathogenic bacteria and opportunistic parasites.	
Thesaurus Nal: 	Antibacterial plants; Antileishmanials; Nanoparticles.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agroenergia (CNPAE)	
Exemplares: 	CNPAE - (4384-1) - UPC - AP - DD -  -  -  - DIS