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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
08/03/2010 |
Data da última atualização: |
03/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FUGANTI, R.; MACHADO, M. de F. P. da S.; LOPES, V. S.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; ABDELNOOR, R. V.; MARCELINO, F. C.; NEPOMUCENO, A. L. |
Afiliação: |
RENATA FUGANTI, UEM; MARIA DE FÁTIMA PIRES DA SILVA MACHADO, UEM; VALÉRIA STEFANIA LOPES, UNIFIL; JOAO FLAVIO VELOSO SILVA, CPAMT; CARLOS ALBERTO ARRABAL ARIAS, CNPSo; SILVANA REGINA ROCKENBACH MARIN, CNPSo; ELISEU BINNECK, CNPSo; RICARDO VILELA ABDELNOOR, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSo; ALEXANDRE LIMA NEPOMUCENO, CNPSo. |
Título: |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Journal of Biomedicine and Biotechnology, 2010. |
Páginas: |
9 p. |
ISSN: |
1110-7251 |
DOI: |
10.1155/2010/847673 |
Idioma: |
Inglês |
Conteúdo: |
During earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions. |
Palavras-Chave: |
Marcadores moleculares; Nematóide de galha; Soybean. |
Thesagro: |
Meloidogyne Javanica; Nematóide; Soja. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162362/1/847673-1.pdf
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Marc: |
LEADER 02071naa a2200337 a 4500 001 1660121 005 2017-08-03 008 2010 bl uuuu u00u1 u #d 022 $a1110-7251 024 7 $a10.1155/2010/847673$2DOI 100 1 $aFUGANTI, R. 245 $aSize of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. 260 $c2010 300 $a9 p. 520 $aDuring earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions. 650 $aMeloidogyne Javanica 650 $aNematóide 650 $aSoja 653 $aMarcadores moleculares 653 $aNematóide de galha 653 $aSoybean 700 1 $aMACHADO, M. de F. P. da S. 700 1 $aLOPES, V. S. 700 1 $aSILVA, J. F. V. 700 1 $aARIAS, C. A. A. 700 1 $aMARIN, S. R. R. 700 1 $aBINNECK, E. 700 1 $aABDELNOOR, R. V. 700 1 $aMARCELINO, F. C. 700 1 $aNEPOMUCENO, A. L. 773 $tJournal of Biomedicine and Biotechnology, 2010.
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Embrapa Soja (CNPSO) |
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Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
28/03/2006 |
Data da última atualização: |
28/03/2006 |
Autoria: |
VIEIRA, D. C.; ASSIS, O. B. G.; BERNARDES-FILHO, R.; CAMPANA-FILHO, S. P. |
Título: |
Pore-size estimation of chitosan thin-film by atomoc force microscopy. |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Acta Microscopica, Rio de Janeiro, v.8, p. 317-318, Oct. 1999. Supplement A. Edição de Proceedings of the XVII Congress of the Brazilian Society for Microscopy and Microanalysis; X Congress of the Brazilian Society for Cell Biology held in Santos-SP, October 13-16, 1999. Resumo. |
Idioma: |
Inglês |
Conteúdo: |
Chitosan (CHI) is a polysaccharide derived from chitin, the second most abundant renewable organic compound on earth next to cellulose. Chitin found in crustacean shells, insect exoskeletons and plankton, and when deacetylated (which attain around 80% of the units) generates the chitosan. CHI is composed primarily of repeating units of beta (1-4) 2-amino-2-deoxy-D-glucose (or D-glucosamine) whose structure is similar to cellulose with the hydroxy groups in position 2 replaced by acetylamino groups. |
Palavras-Chave: |
CHI; Polissacarídeos. |
Thesagro: |
Celulose. |
Thesaurus NAL: |
chitosan. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01286naa a2200205 a 4500 001 1029755 005 2006-03-28 008 1999 bl --- 0-- u #d 100 1 $aVIEIRA, D. C. 245 $aPore-size estimation of chitosan thin-film by atomoc force microscopy. 260 $c1999 520 $aChitosan (CHI) is a polysaccharide derived from chitin, the second most abundant renewable organic compound on earth next to cellulose. Chitin found in crustacean shells, insect exoskeletons and plankton, and when deacetylated (which attain around 80% of the units) generates the chitosan. CHI is composed primarily of repeating units of beta (1-4) 2-amino-2-deoxy-D-glucose (or D-glucosamine) whose structure is similar to cellulose with the hydroxy groups in position 2 replaced by acetylamino groups. 650 $achitosan 650 $aCelulose 653 $aCHI 653 $aPolissacarídeos 700 1 $aASSIS, O. B. G. 700 1 $aBERNARDES-FILHO, R. 700 1 $aCAMPANA-FILHO, S. P. 773 $tActa Microscopica, Rio de Janeiro$gv.8, p. 317-318, Oct. 1999. Supplement A. Edição de Proceedings of the XVII Congress of the Brazilian Society for Microscopy and Microanalysis; X Congress of the Brazilian Society for Cell Biology held in Santos-SP, October 13-16, 1999. Resumo.
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