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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
19/02/2021 |
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Data da última atualização: |
07/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARRAES, F. B. M.; MARTINS-DE-SA, D; VASQUEZ, D. D. N.; MELO, B. P.; FAHEEM, M.; MACEDO, L. L. P. de; MORGANTE, C. V.; BARBOSA, J. A. R. G.; TOGAWA, R. C.; MOREIRA, V. J. V.; DANCHIN, E. G. J.; SA, M. F. G. de. |
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Afiliação: |
FABRICIO BARBOSA MONTEIRO ARRAES; DIOGO MARTINS-DE-SA; DANIEL D. NORIEGA VASQUEZ; BRUNO PAES MELO; MUHAMMAD FAHEEM; LEONARDO LIMA PEPINO DE MACEDO; CAROLINA VIANNA MORGANTE, CPATSA; JOÃO ALEXANDRE R. G. BARBOSA; ROBERTO COITI TOGAWA; VALDEIR JUNIO VAZ MOREIRA; ETIENNE G. J. DANCHIN; MARIA FATIMA GROSSI DE SA. |
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Título: |
Dissecting protein domain variability in the core rna interference machinery of five insect orders. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
RNA Biology, v. 18, n. 11, p. 1653-1681, 2021. |
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DOI: |
https://doi.org/10.1080/15476286.2020.1861816 |
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Idioma: |
Inglês |
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Conteúdo: |
RNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plants and viruses. In conclusion, our results highlight several variability hotspots that deserve further investigation in order to improve the application of RNAi technology in the control of insect pests. MenosRNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plant... Mostrar Tudo |
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Palavras-Chave: |
Argonaute; DCR1; Dicer; Drosha; DsRBDs; Evolução da proteína; In silico analysis; Loquacious; Pasha; Protein evolution; R2D2; RIIID II; Sequência de proteínas inteira; Structure-function relationship. |
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Thesagro: |
Controle Genético; Genoma; Inseto; Praga; Proteína. |
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Thesaurus Nal: |
Insect control; Pest control; Protein structure; Proteins. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/230917/1/Dissecting-protein-domain-variability-2021.pdf
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Marc: |
LEADER 03184naa a2200541 a 4500
001 2130159
005 2022-02-07
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/15476286.2020.1861816$2DOI
100 1 $aARRAES, F. B. M.
245 $aDissecting protein domain variability in the core rna interference machinery of five insect orders.$h[electronic resource]
260 $c2021
520 $aRNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plants and viruses. In conclusion, our results highlight several variability hotspots that deserve further investigation in order to improve the application of RNAi technology in the control of insect pests.
650 $aInsect control
650 $aPest control
650 $aProtein structure
650 $aProteins
650 $aControle Genético
650 $aGenoma
650 $aInseto
650 $aPraga
650 $aProteína
653 $aArgonaute
653 $aDCR1
653 $aDicer
653 $aDrosha
653 $aDsRBDs
653 $aEvolução da proteína
653 $aIn silico analysis
653 $aLoquacious
653 $aPasha
653 $aProtein evolution
653 $aR2D2
653 $aRIIID II
653 $aSequência de proteínas inteira
653 $aStructure-function relationship
700 1 $aMARTINS-DE-SA, D
700 1 $aVASQUEZ, D. D. N.
700 1 $aMELO, B. P.
700 1 $aFAHEEM, M.
700 1 $aMACEDO, L. L. P. de
700 1 $aMORGANTE, C. V.
700 1 $aBARBOSA, J. A. R. G.
700 1 $aTOGAWA, R. C.
700 1 $aMOREIRA, V. J. V.
700 1 $aDANCHIN, E. G. J.
700 1 $aSA, M. F. G. de
773 $tRNA Biology$gv. 18, n. 11, p. 1653-1681, 2021.
Download
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
01/09/2017 |
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Data da última atualização: |
01/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUZA, J. D. de; SILVA, E. M. de A.; COELHO FILHO, M. A.; MORILLON, R.; BONATTO, D.; MICHELI, F.; GESTEIRA, A. da S. |
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Afiliação: |
JOADSON DUTRA DE SOUZA; EDSON MARIO DE ANDRADE SILVA; MAURICIO ANTONIO COELHO FILHO, CNPMF; RAPHAËL MORILLON; DIEGO BONATTO; FABIENNE MICHELI; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Different adaptation strategies of two citrus scion/rootstock Combinations in response to drought stress. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
PLOS ONE, May, 2017, |
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ISSN: |
1932-6203 |
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Idioma: |
Inglês |
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Conteúdo: |
Scion/rootstock interaction is important for plant development and for breeding programs. In this context, polyploid rootstocks presented several advantages, mainly in relation to biotic and abiotic stresses. Here we analyzed the response to drought of two different scion/rootstock combinations presenting different polyploidy: the diploid (2x) and autotetraploid (4x) Rangpur lime (Citrus limonia, Osbeck) rootstocks grafted with 2x Valencia Delta sweet orange (Citrus sinensis) scions, named V/2xRL and V/4xRL, respectively. Based on previous gene expression data, we developed an interactomic approach to identify proteins involved in V/2xRL and V/4xRL response to drought. A main interactomic network containing 3,830 nodes and 97,652 edges was built from V/2xRL and V/4xRL data. Exclusive proteins of the V/2xRL and V/4xRL networks (2,056 and 1,001, respectively), as well as common to both networks (773) were identified. Functional clusters were obtained and two models of drought stress response for the V/2xRL and V/4xRL genotypes were designed. Even if the V/2xRL plant implement some tolerance mechanisms, the global plant response to drought was rapid and quickly exhaustive resulting in a general tendency to dehydration avoidance, which presented some advantage in short and strong drought stress conditions, but which, in long terms, does not allow the plant survival. At the contrary, the V/4xRL plants presented a response which strong impacts on development but that present some advantages in case of prolonged drought. Finally, some specific proteins, which presented high centrality on interactomic analysis were identified as good candidates for subsequent functional analysis of citrus genes related to drought response, as well as be good markers of one or another physiological mechanism implemented by the plants. MenosScion/rootstock interaction is important for plant development and for breeding programs. In this context, polyploid rootstocks presented several advantages, mainly in relation to biotic and abiotic stresses. Here we analyzed the response to drought of two different scion/rootstock combinations presenting different polyploidy: the diploid (2x) and autotetraploid (4x) Rangpur lime (Citrus limonia, Osbeck) rootstocks grafted with 2x Valencia Delta sweet orange (Citrus sinensis) scions, named V/2xRL and V/4xRL, respectively. Based on previous gene expression data, we developed an interactomic approach to identify proteins involved in V/2xRL and V/4xRL response to drought. A main interactomic network containing 3,830 nodes and 97,652 edges was built from V/2xRL and V/4xRL data. Exclusive proteins of the V/2xRL and V/4xRL networks (2,056 and 1,001, respectively), as well as common to both networks (773) were identified. Functional clusters were obtained and two models of drought stress response for the V/2xRL and V/4xRL genotypes were designed. Even if the V/2xRL plant implement some tolerance mechanisms, the global plant response to drought was rapid and quickly exhaustive resulting in a general tendency to dehydration avoidance, which presented some advantage in short and strong drought stress conditions, but which, in long terms, does not allow the plant survival. At the contrary, the V/4xRL plants presented a response which strong impacts on development but that present some ... Mostrar Tudo |
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Thesagro: |
Fruta Cítrica. |
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Thesaurus NAL: |
Citrus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163348/1/Different-adaptation-strategies-Dutra-et-al-2017-PlosOne.pdf
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Marc: |
LEADER 02484naa a2200229 a 4500
001 2074913
005 2017-09-01
008 2017 bl uuuu u00u1 u #d
022 $a1932-6203
100 1 $aSOUZA, J. D. de
245 $aDifferent adaptation strategies of two citrus scion/rootstock Combinations in response to drought stress.$h[electronic resource]
260 $c2017
520 $aScion/rootstock interaction is important for plant development and for breeding programs. In this context, polyploid rootstocks presented several advantages, mainly in relation to biotic and abiotic stresses. Here we analyzed the response to drought of two different scion/rootstock combinations presenting different polyploidy: the diploid (2x) and autotetraploid (4x) Rangpur lime (Citrus limonia, Osbeck) rootstocks grafted with 2x Valencia Delta sweet orange (Citrus sinensis) scions, named V/2xRL and V/4xRL, respectively. Based on previous gene expression data, we developed an interactomic approach to identify proteins involved in V/2xRL and V/4xRL response to drought. A main interactomic network containing 3,830 nodes and 97,652 edges was built from V/2xRL and V/4xRL data. Exclusive proteins of the V/2xRL and V/4xRL networks (2,056 and 1,001, respectively), as well as common to both networks (773) were identified. Functional clusters were obtained and two models of drought stress response for the V/2xRL and V/4xRL genotypes were designed. Even if the V/2xRL plant implement some tolerance mechanisms, the global plant response to drought was rapid and quickly exhaustive resulting in a general tendency to dehydration avoidance, which presented some advantage in short and strong drought stress conditions, but which, in long terms, does not allow the plant survival. At the contrary, the V/4xRL plants presented a response which strong impacts on development but that present some advantages in case of prolonged drought. Finally, some specific proteins, which presented high centrality on interactomic analysis were identified as good candidates for subsequent functional analysis of citrus genes related to drought response, as well as be good markers of one or another physiological mechanism implemented by the plants.
650 $aCitrus
650 $aFruta Cítrica
700 1 $aSILVA, E. M. de A.
700 1 $aCOELHO FILHO, M. A.
700 1 $aMORILLON, R.
700 1 $aBONATTO, D.
700 1 $aMICHELI, F.
700 1 $aGESTEIRA, A. da S.
773 $tPLOS ONE, May, 2017
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Embrapa Mandioca e Fruticultura (CNPMF) |
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