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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
08/04/1999 |
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Data da última atualização: |
17/06/2019 |
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Autoria: |
CENTELLAS, A. Q.; FORTES, G. R. de L.; MULLER, N. T. G.; ZANOL, G. C.; FLORES, R.; GOTTINARI, R. A. |
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Afiliação: |
ALBERTO QUEZADA CENTELLAS, Universidade Federal de Pelotas - UFPel; GERSON RENAN DE LUCES FORTES, CPACT; NILVANE TEREZINHA GHELLAR MÜLLER, Universidade Federal de Pelotas - UFPel; GENI CARMEN ZANOL, CPACT; REJANE FLORES, Universidade Federal de Pelotas - UFPel; ROSETE APARECIDA GOTTINAR, Universidade Federal de Pelotas - UFPel. |
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Título: |
Efeito de auxinas sintéticas no enraizamento in vitro da macieira. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 2, p. 181-86, fev. 1999 |
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Idioma: |
Português |
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Notas: |
Título em inglês: Effects of synthetic auxins on the in vitro rooting of apple tree. |
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Conteúdo: |
Brotacoes de macieira (Malus domestica, Borkh), cv. Fred Hough, oriundas do processo de multiplicacao in vitro, foram inoculadas em meio MS e MS/2, testando-se os reguladores de crescimento: acido indol-3-acetico (AIA); acido indolbutirico (AIB) e acido naftaleno acetico (ANA), nas concentracoes de 0, 1, 3 e 5 UM com o objetivo de observar o efeito dessas auxinas sobre o enraizamento da cultivar. Foram acrescentadas aos meios as vitaminas MS mio-inositol (100 mg/L) e sacarose (30 g/L) em meio de agar (6 g/L). O pH do meio foi ajustado para 5,8 e a cultura foi incubada a 25 +- 2 Graus C. e 16 horas de fotoperiodo a 2.000 lux, permanecendo por 30 dias. Os tratamentos foram repetidos cinco vezes e cada repeticao constou de cinco explantes inoculados em frasco de 250 mL contendo 40 mL do meio. O meio MS/2 em todas as concentracoes testadas foi melhor que o MS. O ANA e o AIB, ambos na concentracao de 3 UM, em meio MS/2, tiveram comportamento semelhante na porcentagem de enraizamento e no numero de raizes produzidas; no entanto, o ANA provocou efeitos indesejaveis na qualidade destas, havendo formacao de calo na base das brotacoes e raizes grossas. O AIA obteve melhor resposta nas altas concentracoes, mas nao foi melhor que o AIB e ANA. |
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Palavras-Chave: |
Acido indol-3-acetico; Acido Naftaleno acetico; Growth substances; Indol acetic acid; Indol butiric acid; Naphtalene acetic acid; Reguladores de crescimento. |
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Thesagro: |
Ácido Indolbutírico; Malus Domestica; Micropropagação. |
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Thesaurus Nal: |
micropropagation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/5914/1/pab360_96.pdf
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Marc: |
LEADER 02288naa a2200325 a 4500
001 1089484
005 2019-06-17
008 1999 bl uuuu u00u1 u #d
100 1 $aCENTELLAS, A. Q.
245 $aEfeito de auxinas sintéticas no enraizamento in vitro da macieira.
260 $c1999
500 $aTítulo em inglês: Effects of synthetic auxins on the in vitro rooting of apple tree.
520 $aBrotacoes de macieira (Malus domestica, Borkh), cv. Fred Hough, oriundas do processo de multiplicacao in vitro, foram inoculadas em meio MS e MS/2, testando-se os reguladores de crescimento: acido indol-3-acetico (AIA); acido indolbutirico (AIB) e acido naftaleno acetico (ANA), nas concentracoes de 0, 1, 3 e 5 UM com o objetivo de observar o efeito dessas auxinas sobre o enraizamento da cultivar. Foram acrescentadas aos meios as vitaminas MS mio-inositol (100 mg/L) e sacarose (30 g/L) em meio de agar (6 g/L). O pH do meio foi ajustado para 5,8 e a cultura foi incubada a 25 +- 2 Graus C. e 16 horas de fotoperiodo a 2.000 lux, permanecendo por 30 dias. Os tratamentos foram repetidos cinco vezes e cada repeticao constou de cinco explantes inoculados em frasco de 250 mL contendo 40 mL do meio. O meio MS/2 em todas as concentracoes testadas foi melhor que o MS. O ANA e o AIB, ambos na concentracao de 3 UM, em meio MS/2, tiveram comportamento semelhante na porcentagem de enraizamento e no numero de raizes produzidas; no entanto, o ANA provocou efeitos indesejaveis na qualidade destas, havendo formacao de calo na base das brotacoes e raizes grossas. O AIA obteve melhor resposta nas altas concentracoes, mas nao foi melhor que o AIB e ANA.
650 $amicropropagation
650 $aÁcido Indolbutírico
650 $aMalus Domestica
650 $aMicropropagação
653 $aAcido indol-3-acetico
653 $aAcido Naftaleno acetico
653 $aGrowth substances
653 $aIndol acetic acid
653 $aIndol butiric acid
653 $aNaphtalene acetic acid
653 $aReguladores de crescimento
700 1 $aFORTES, G. R. de L.
700 1 $aMULLER, N. T. G.
700 1 $aZANOL, G. C.
700 1 $aFLORES, R.
700 1 $aGOTTINARI, R. A.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 34, n. 2, p. 181-86, fev. 1999
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
09/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OTTO, P. I.; GUIMARÃES, S. E. F.; VERARDO, L. L.; AZEVEDO, A. L. S.; VANDENPLAS, J.; SEVILLANO, C. A.; MARQUES, D. B. D.; PIRES, M. de F. A.; FREITAS, C. de; VERNEQUE, R. da S.; MARTINS, M. F.; PANETTO, J. C. do C.; CARVALHO, W. A.; GOBO, D. O. R.; SILVA, M. V. G. B.; MACHADO, M. A. |
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Afiliação: |
ANA LUISA SOUSA AZEVEDO, CNPGL; MARIA DE FATIMA AVILA PIRES, CNPGL; CELIO DE FREITAS, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; MARTA FONSECA MARTINS, CNPGL; JOAO CLAUDIO DO CARMO PANETTO, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARCO ANTONIO MACHADO, CNPGL. |
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Título: |
Genome-wide association studies for heat stress response in Bos taurus × Bos indicus crossbred cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 102, n. 9, p. 8148-8158, 2019. |
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DOI: |
https://doi.org/10.3168/jds.2018-15305 |
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Idioma: |
Inglês |
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Conteúdo: |
Heat stress is an important issue in the global dairy industry. In tropical areas, an alternative to overcome heat stress is the use of crossbred animals or synthetic breeds, such as the Girolando. In this study, we performed a genome-wide association study (GWAS) and post-GWAS analyses for heat stress in an experimental Gir × Holstein F2 population. Rectal temperature (RT) was measured in heat-stressed F2 animals, and the variation between 2 consecutive RT measurements (ΔRT) was used as the dependent variable. Illumina BovineSNP50v1 BeadChip (Illumina Inc., San Diego, CA) and single-SNP approach were used for GWAS. Post-GWAS analyses were performed by gene ontology terms enrichment and gene-transcription factor (TF) networks, generated from enriched TF. The breed origin of marker alleles in the F2 population was assigned using the breed of origin of alleles (BOA) approach. Heritability and repeatability estimates (± standard error) for ΔRT were 0.13 ± 0.08 and 0.29 ± 0.06, respectively. Association analysis revealed 6 SNP significantly associated with ΔRT. Genes involved with biological processes in response to heat stress effects (LIF, OSM, TXNRD2, and DGCR8) were identified as putative candidate genes. After performing the BOA approach, the 10% of F2 animals with the lowest breeding values for ΔRT were classified as low-ΔRT, and the 10% with the highest breeding values for ΔRT were classified as high-ΔRT. On average, 49.4% of low-ΔRT animals had 2 alleles from the Holstein breed (HH), and 39% had both alleles from the Gir breed (GG). In high-ΔRT animals, the average proportion of animals for HH and GG were 1.4 and 50.2%, respectively. This study allowed the identification of candidate genes for ΔRT in Gir × Holstein crossbred animals. According to the BOA approach, Holstein breed alleles could be associated with better response to heat stress effects, which could be explained by the fact that Holstein animals are more affected by heat stress than Gir animals and thus require a genetic architecture to defend the body from the deleterious effects of heat stress. Future studies can provide further knowledge to uncover the genetic architecture underlying heat stress in crossbred cattle. MenosHeat stress is an important issue in the global dairy industry. In tropical areas, an alternative to overcome heat stress is the use of crossbred animals or synthetic breeds, such as the Girolando. In this study, we performed a genome-wide association study (GWAS) and post-GWAS analyses for heat stress in an experimental Gir × Holstein F2 population. Rectal temperature (RT) was measured in heat-stressed F2 animals, and the variation between 2 consecutive RT measurements (ΔRT) was used as the dependent variable. Illumina BovineSNP50v1 BeadChip (Illumina Inc., San Diego, CA) and single-SNP approach were used for GWAS. Post-GWAS analyses were performed by gene ontology terms enrichment and gene-transcription factor (TF) networks, generated from enriched TF. The breed origin of marker alleles in the F2 population was assigned using the breed of origin of alleles (BOA) approach. Heritability and repeatability estimates (± standard error) for ΔRT were 0.13 ± 0.08 and 0.29 ± 0.06, respectively. Association analysis revealed 6 SNP significantly associated with ΔRT. Genes involved with biological processes in response to heat stress effects (LIF, OSM, TXNRD2, and DGCR8) were identified as putative candidate genes. After performing the BOA approach, the 10% of F2 animals with the lowest breeding values for ΔRT were classified as low-ΔRT, and the 10% with the highest breeding values for ΔRT were classified as high-ΔRT. On average, 49.4% of low-ΔR... Mostrar Tudo |
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Palavras-Chave: |
Crossbred cattle; Gene network; Post-GWAS analyses. |
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Thesaurus NAL: |
Heat stress. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03369naa a2200361 a 4500
001 2118465
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2018-15305$2DOI
100 1 $aOTTO, P. I.
245 $aGenome-wide association studies for heat stress response in Bos taurus × Bos indicus crossbred cattle.$h[electronic resource]
260 $c2019
520 $aHeat stress is an important issue in the global dairy industry. In tropical areas, an alternative to overcome heat stress is the use of crossbred animals or synthetic breeds, such as the Girolando. In this study, we performed a genome-wide association study (GWAS) and post-GWAS analyses for heat stress in an experimental Gir × Holstein F2 population. Rectal temperature (RT) was measured in heat-stressed F2 animals, and the variation between 2 consecutive RT measurements (ΔRT) was used as the dependent variable. Illumina BovineSNP50v1 BeadChip (Illumina Inc., San Diego, CA) and single-SNP approach were used for GWAS. Post-GWAS analyses were performed by gene ontology terms enrichment and gene-transcription factor (TF) networks, generated from enriched TF. The breed origin of marker alleles in the F2 population was assigned using the breed of origin of alleles (BOA) approach. Heritability and repeatability estimates (± standard error) for ΔRT were 0.13 ± 0.08 and 0.29 ± 0.06, respectively. Association analysis revealed 6 SNP significantly associated with ΔRT. Genes involved with biological processes in response to heat stress effects (LIF, OSM, TXNRD2, and DGCR8) were identified as putative candidate genes. After performing the BOA approach, the 10% of F2 animals with the lowest breeding values for ΔRT were classified as low-ΔRT, and the 10% with the highest breeding values for ΔRT were classified as high-ΔRT. On average, 49.4% of low-ΔRT animals had 2 alleles from the Holstein breed (HH), and 39% had both alleles from the Gir breed (GG). In high-ΔRT animals, the average proportion of animals for HH and GG were 1.4 and 50.2%, respectively. This study allowed the identification of candidate genes for ΔRT in Gir × Holstein crossbred animals. According to the BOA approach, Holstein breed alleles could be associated with better response to heat stress effects, which could be explained by the fact that Holstein animals are more affected by heat stress than Gir animals and thus require a genetic architecture to defend the body from the deleterious effects of heat stress. Future studies can provide further knowledge to uncover the genetic architecture underlying heat stress in crossbred cattle.
650 $aHeat stress
653 $aCrossbred cattle
653 $aGene network
653 $aPost-GWAS analyses
700 1 $aGUIMARÃES, S. E. F.
700 1 $aVERARDO, L. L.
700 1 $aAZEVEDO, A. L. S.
700 1 $aVANDENPLAS, J.
700 1 $aSEVILLANO, C. A.
700 1 $aMARQUES, D. B. D.
700 1 $aPIRES, M. de F. A.
700 1 $aFREITAS, C. de
700 1 $aVERNEQUE, R. da S.
700 1 $aMARTINS, M. F.
700 1 $aPANETTO, J. C. do C.
700 1 $aCARVALHO, W. A.
700 1 $aGOBO, D. O. R.
700 1 $aSILVA, M. V. G. B.
700 1 $aMACHADO, M. A.
773 $tJournal of Dairy Science$gv. 102, n. 9, p. 8148-8158, 2019.
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