Data/hora: 13/06/2024 07:49:22

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 13/05/2016
Data da última atualização: | 27/07/2016
Autoria: | MONJE, J.; LUNDBERG, D. S.; LAZAROVITS, G.; REIS, V. M.
Afiliação: | JOHNSTON MONGE, UNIVERSITY OF GUELPH,ONTÁRIO, CA; DEREK S. LUNDBERG, MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY, GE; GEORGE LAZAROVITS, A&L BIOLOGICALS, AGROECOLOGY RESEARCH SERVICES CENTRE, ONTÁRIO, CA; VERONICA MASSENA REIS, CNPAB.
Título: | Bacterial populations in juvenile maize rhizospheres originate from both seed and soil
Ano de publicação: | 2016
Fonte/Imprenta: | Plant and Soil, v. 405, p. 447-355, 2016
ISSN: | 1573-5036
DOI: | 10.1007/s11104-016-2826-0
Idioma: | Inglês

Conteúdo:

Background and aims: To assess the impacts of soil microbes and plant genotype on the composition of maize associated bacterial communities.
Methods: Two genotypes of Brazilian maize were planted indoors on sterile sand, a deep underground subsoil, and a nutrient-rich topsoil from the Amazon jungle (terra preta). DNA was extracted from rhizospheres, phyllospheres, and surface sterilized roots for 16S rDNA fingerprinting and next generation sequencing.
Results: Neither plant genotype nor soil type appeared to influence bacterial diversity in phyllospheres or endospheres. Rhizospheres showed strikingly similar 16S rDNA ordination of both fingerprinting and sequencing data, with soil type driving grouping patterns and genotype having a significant impact only on sterile sand. Rhizospheres grown in non-sterile soils contained greater bacterial diversity than sterile-sand grown ones, however the dominant OTUs (species of Proteobacteria and Bacteroidetes) were found in all rhizospheres suggesting seeds as a common source of inoculum. Rhizospheres of the commercial hybrid appeared to contain less bacterial diversity than the landrace.
Conclusions: Maize rhizospheres receive diverse bacteria from soil, are influenced by the genotype or treatment of the seed, and are dominated by species of Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. As many dominant 16S rDNA sequences were observed in rhizospheres grown in both sterile and non-sterile substrate, we conclude that the most common bacterial cells in juvenile maize rhizospheres are seed transmitted.

Palavras-Chave: | Endophyte; Epiphyte; Inoculation; Next-generation sequencing.
Thesagro: | Zea Mays.
Thesaurus Nal: | Microbial ecology; Rhizosphere.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (40218-1) - UPC - SP - PP - 2016.00070 -  - 2016.00070 - DIS