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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
23/03/2016 |
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Data da última atualização: |
23/03/2016 |
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Autoria: |
ALCÂNTARA, B. K. de; ALFIERI, A. A.; HEADLEY, S. A.; RODRIGUES, W. B.; OTONEL, R. A. A.; LUNARDI, M.; ALFIERI, A. F. |
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Afiliação: |
BRÍGIDA K. DE ALCÂNTARA, DMVP/UEL; AMAURI A. ALFIERI, DMVP/UEL; SELWYN A. HEADLEY, DMVP/UEL; WAGNER B. RODRIGUES, DMVP/UEL; RODRIGO A. A. OTONEL, DMVP/UEL; MICHELE LUNARDI, UNIC; ALICE F. ALFIERI, DMVP/UEL. |
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Título: |
Molecular characterization of bovine Deltapapillomavirus (BPV1, 2, and 13) DNA in equine sarcoids. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 431-436, maio 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Sarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreover, these findings represent the first description of three types of BPV diagnosed in the same horse, as well as the first confirmation of BPV1 and 2 in horses from Brazil. MenosSarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreove... Mostrar Tudo |
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Palavras-Chave: |
Bovine Deltapapillomavirus; Deltapapillomavirus bovino; Equine sarcoids; Horse; Papillomavirus; Sarcoide equino; Tumor de pele. |
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Thesagro: |
DNA. |
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Thesaurus Nal: |
Papillomaviridae; Skin neoplasms. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141614/1/Molecular-characterization.pdf
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Marc: |
LEADER 02588naa a2200313 a 4500
001 2041730
005 2016-03-23
008 2015 bl uuuu u00u1 u #d
100 1 $aALCÂNTARA, B. K. de
245 $aMolecular characterization of bovine Deltapapillomavirus (BPV1, 2, and 13) DNA in equine sarcoids.
260 $c2015
520 $aSarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreover, these findings represent the first description of three types of BPV diagnosed in the same horse, as well as the first confirmation of BPV1 and 2 in horses from Brazil.
650 $aPapillomaviridae
650 $aSkin neoplasms
650 $aDNA
653 $aBovine Deltapapillomavirus
653 $aDeltapapillomavirus bovino
653 $aEquine sarcoids
653 $aHorse
653 $aPapillomavirus
653 $aSarcoide equino
653 $aTumor de pele
700 1 $aALFIERI, A. A.
700 1 $aHEADLEY, S. A.
700 1 $aRODRIGUES, W. B.
700 1 $aOTONEL, R. A. A.
700 1 $aLUNARDI, M.
700 1 $aALFIERI, A. F.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 35, n. 5, p. 431-436, maio 2015.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
07/12/2018 |
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Data da última atualização: |
17/12/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FARIA, J. C. de; ARAGÃO, F. J. L. |
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Afiliação: |
JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
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Título: |
RNAi based transgenic common bean resistant to Bean golden mosaic virus. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: SIMPOSIO DE MANEJO DE DOENÇAS DE PLANTAS, 18., 2018, Lavras. Biotecnologia aplicada à fitopatologia: anais. Lavras: Ed. UFLA, 2018. |
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Páginas: |
p. 90-106. |
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Idioma: |
Inglês |
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Thesagro: |
Planta Transgênica; Variedade Resistente; Vírus. |
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Thesaurus NAL: |
Bean golden mosaic virus; Beans; Begomovirus; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 00701nam a2200205 a 4500
001 2100962
005 2018-12-17
008 2018 bl uuuu u00u1 u #d
100 1 $aFARIA, J. C. de
245 $aRNAi based transgenic common bean resistant to Bean golden mosaic virus.$h[electronic resource]
260 $aIn: SIMPOSIO DE MANEJO DE DOENÇAS DE PLANTAS, 18., 2018, Lavras. Biotecnologia aplicada à fitopatologia: anais. Lavras: Ed. UFLA$c2018
300 $ap. 90-106.
650 $aBean golden mosaic virus
650 $aBeans
650 $aBegomovirus
650 $aTransgenic plants
650 $aPlanta Transgênica
650 $aVariedade Resistente
650 $aVírus
700 1 $aARAGÃO, F. J. L.
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