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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
16/04/2013 |
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Data da última atualização: |
06/08/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVEIRA, J. A. G.; RABELO, E. M. L.; LACERDA, A. C. R.; BORGES, P. A. L.; TOMAS, W. M.; PELLEGRIN, A. O.; TOMICH, R. G. P.; RIBEIRO, M. F. B. |
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Afiliação: |
JÚLIA A. G. SILVEIRA, UFMG; ÉLIDA M. L. RABELO, UFMG; ANA C. R. LACERDA, Embrapa Pantanal; PAULO A. L. BORGES, Embrapa Pantanal; WALFRIDO MORAES TOMAS, CPAP; AIESCA OLIVEIRA PELLEGRIN, CPAP; RENATA R. G. TOMICH, Embrapa Pantanal; MÚCIO F. B. RIBEIRO, UFMG. |
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Título: |
Molecular detection and identification of hemoparasites in pampas deer (Ozotoceros bezoarticus Linnaeus, 1758) from the Pantanal Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 4, n. 4, p. 341-345, 2013. |
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ISSN: |
1877-959X |
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Idioma: |
Inglês |
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Conteúdo: |
Hemoparasites were surveyed in 60 free-living pampas deer Ozotoceros bezoarticus from the central area of the Pantanal, known as Nhecolândia, State of Mato Grosso do Sul, Brazil, through the analysis of nested PCR assays and nucleotide sequencing. Blood samples were tested for Babesia/Theileria, Anaplasma spp., and Trypanosoma spp. using nPCR assays and sequencing of the 18S rRNA, msp4, ITS, and cathepsin L genes. The identity of each sequence was confirmed by comparison with sequences from GenBank using BLAST software. Forty-six (77%) pampas deer were positive for at least one hemoparasite, according to PCR assays. Co-infection occurred in 13 (22%) animals. Based on the sequencing results, 29 (48%) tested positive for A. marginale. Babesia/Theileria were detected in 23 (38%) samples, and according to the sequencing results 52% (12/23) of the samples were similar to T. cervi, 13% (3/23) were similar to Babesia bovis, and 9% (2/23) were similar to B. bigemina. No samples were amplified with the primers for T. vivax, while 11 (18%) were amplified with the ITS primers for T. evansi. The results showed pampas deer to be co-infected with several hemoparasites, including species that may cause serious disease in cattle. Pampas deer is an endangered species in Brazil, and the consequences of these infections to their
health are poorly understood. |
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Palavras-Chave: |
Brazilian deer; Hemoparasites; Molecular detection. |
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Thesagro: |
Ozotoceros Bezoarticus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02178naa a2200265 a 4500
001 1956008
005 2013-08-06
008 2013 bl --- 0-- u #d
022 $a1877-959X
100 1 $aSILVEIRA, J. A. G.
245 $aMolecular detection and identification of hemoparasites in pampas deer (Ozotoceros bezoarticus Linnaeus, 1758) from the Pantanal Brazil.
260 $c2013
520 $aHemoparasites were surveyed in 60 free-living pampas deer Ozotoceros bezoarticus from the central area of the Pantanal, known as Nhecolândia, State of Mato Grosso do Sul, Brazil, through the analysis of nested PCR assays and nucleotide sequencing. Blood samples were tested for Babesia/Theileria, Anaplasma spp., and Trypanosoma spp. using nPCR assays and sequencing of the 18S rRNA, msp4, ITS, and cathepsin L genes. The identity of each sequence was confirmed by comparison with sequences from GenBank using BLAST software. Forty-six (77%) pampas deer were positive for at least one hemoparasite, according to PCR assays. Co-infection occurred in 13 (22%) animals. Based on the sequencing results, 29 (48%) tested positive for A. marginale. Babesia/Theileria were detected in 23 (38%) samples, and according to the sequencing results 52% (12/23) of the samples were similar to T. cervi, 13% (3/23) were similar to Babesia bovis, and 9% (2/23) were similar to B. bigemina. No samples were amplified with the primers for T. vivax, while 11 (18%) were amplified with the ITS primers for T. evansi. The results showed pampas deer to be co-infected with several hemoparasites, including species that may cause serious disease in cattle. Pampas deer is an endangered species in Brazil, and the consequences of these infections to their health are poorly understood.
650 $aOzotoceros Bezoarticus
653 $aBrazilian deer
653 $aHemoparasites
653 $aMolecular detection
700 1 $aRABELO, E. M. L.
700 1 $aLACERDA, A. C. R.
700 1 $aBORGES, P. A. L.
700 1 $aTOMAS, W. M.
700 1 $aPELLEGRIN, A. O.
700 1 $aTOMICH, R. G. P.
700 1 $aRIBEIRO, M. F. B.
773 $tTicks and Tick-borne Diseases$gv. 4, n. 4, p. 341-345, 2013.
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