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| Registros recuperados : 24 | |
| 4. |  | SANTOS, H. F.; LOVATO, L. T.; FLORES, M. L.; TREVISOL, I. M.; LAUTERT, C. Antibodies to specific viruses in backyard poultry from Rio Grande do Sul State, Brazil. In: NATIONAL MEETING OF VIROLOGY, 18., 2007, Búzios, RJ. Anais... Búzios: Sociedade Brasileira de Virologia, 2007. Projeto n. 02.03.21.000-02| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | SANTOS, H. F. dos; FLORES, M. L.; LARA, V. M.; TREVISOL, I. M.; LOVATO, L. T. Anticorpos contra vírus de galinha em cracídeos Ciência Rural, Santa Maria, v.39, n.7, p.2225-2228, out, 2009. Disponível em: . Acesso em: 05 abr. 2010. Projeto/Plano de Ação: 16.00.30004-00| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | MARSARO JÚNIOR, A. L.; NAVIA, D.; QUERINO, R. B.; SOUSA, A. S. R. de; LOVATO, L. Ácaros fitófagos associados a culturas agrícolas e florestais no estado de Roraima. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. ResumoID:937-2.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | MARSARO JUNIOR, A. L.; LOVATO, L.; RONCHI-TELES, B.; SILVA, R. A.; GRIFFREL, S. C. P. Levantamento de hospedeiros e parasitóides de Anastrepha spp. (Diptera: Tephritidae) no município de Boa Vista, Estado de Roraima. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Resumos... Uberlândia, 2008.| Biblioteca(s): Embrapa Roraima. |
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| 15. | | MARSARO JUNIOR, A. L.; LOVATO, L.; RONCHI-TELES, B.; SILVA, R. A. da; GRIFFEL, S. C. P. Levantamento de hospedeiros e parasitóides de Anastrepha spp. (Diptera: Tephritidae) no município de Boa Vista, Estado de Roraima. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: resumos. Viçosa, MG: UFV, 2008. 1 CD ROM. Resumo 937-1.| Biblioteca(s): Embrapa Amapá. |
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| 16. | | GRIFFEL, S. C. P.; MARSARO JUNIOR, A. L.; LOVATO, L.; RONCHI-TELES, B.; SILVA, R. A. da. Ocorrência de Anastrepha spp. (Diptera: Tephritidae) em pomar comercial de goiaba em Roraima. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: resumos. Viçosa: UFV, 2008. 1 CD-ROM. Resumo 930-2.| Biblioteca(s): Embrapa Amapá. |
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| 20. | | CASTRO, A. M. G. de; SOUZA, A. de P.; LOPES, E. S.; SOBRAL, L. F.; LOVATO, L. A.; MENEZES, M.; TISSELLI FILHO, O. Diagnose de deficiência de potássio em folha de milho (Zea mays L.), cv. Piranao pelo teste da putrescina. Piracicaba: ESALQ, 1975. 09p. Trabalho apresentado a Disciplina Nutricao Mineral de Plantas, do Curso de Pos-Graduacao em Solos e Nutricao de Plantas.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
23/10/2019 |
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Data da última atualização: |
12/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; FERNANDA QUEIROS COSTA, UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO; MICHELE MUNK, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; SABINE WOHLRES-VIANA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; RAQUEL VARELA SERAPIÃO, PESAGRO; BRUNO CAMPOS DE CARVALHO, CNPGL; PAULO HENRIQUE CAMPOS JÚNIOR, UFSJ; ALEX CABRAL VIEIRA, UFV; LUIZ ALTAMIRO GARCIA NOGUEIRA, UFF; JOAO HENRIQUE MOREIRA VIANA, CENARGEN. |
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Título: |
Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 54, n. 10, p. 1357-1365, 2019. |
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DOI: |
https://doi.org/10.1111/rda.13544 |
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Idioma: |
Inglês |
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Conteúdo: |
This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav‐age at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes. MenosThis study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav‐age at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and ... Mostrar Tudo |
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Palavras-Chave: |
Activation; Aquaporin 3; Cleavage; Embryos; Heat Shock; Hsp70 Protein; Ionomycin; Maturation. |
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Thesaurus NAL: |
Apoptosis; Blastocyst; Gametocytes; Gene expression; In vitro fertilization; Oocytes; Parthenogenesis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208435/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/203452/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
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Marc: |
LEADER 02828naa a2200421 a 4500
001 2118469
005 2024-04-12
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/rda.13544$2DOI
100 1 $aCAMARGO, L. S. A.
245 $aContrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos.$h[electronic resource]
260 $c2019
520 $aThis study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav‐age at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes.
650 $aApoptosis
650 $aBlastocyst
650 $aGametocytes
650 $aGene expression
650 $aIn vitro fertilization
650 $aOocytes
650 $aParthenogenesis
653 $aActivation
653 $aAquaporin 3
653 $aCleavage
653 $aEmbryos
653 $aHeat Shock
653 $aHsp70 Protein
653 $aIonomycin
653 $aMaturation
700 1 $aCOSTA, F. Q.
700 1 $aMUNK, M.
700 1 $aWOHLRES-VIANA, S.
700 1 $aSERAPIÃO, R. V.
700 1 $aCARVALHO, B. C.
700 1 $aCAMPOS JÚNIOR, P. H.
700 1 $aVIEIRA, A. C.
700 1 $aNOGUEIRA, L. A. G.
700 1 $aVIANA, J. H. M.
773 $tReproduction in Domestic Animals$gv. 54, n. 10, p. 1357-1365, 2019.
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Embrapa Gado de Leite (CNPGL) |
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