| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
06/05/2019 |
|
Data da última atualização: |
06/05/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SANTOS, T. B. dos; SOARES, J. D. M.; LIMA, J. E.; SILVA, J. C.; IVAMOTO, S. T.; BABA, V. Y.; SOUZA, S. G. H.; LORENZETTI, A. P. R.; PASCHOAL, A. R.; MEDA, A. R.; NISHIYAMA JÚNIOR, M. Y.; OLIVEIRA, U. C. de; MOKOCHINSKI, J. B.; GUYOT, R.; JUNQUEIRA-DE-AZEVEDO, I. L. M.; FIGUEIR, A. V. O.; MAZZAFERA, P.; R. JÚNIO, O.; VIEIRA, L. G. E.; PEREIRA, L. F. P.; DOMINGUES, D. S. |
|
Afiliação: |
Tiago Benedito dos Santos, Universidade do Oeste Paulista; João D. M. Soares, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR; Joni E. Lima, Departamento de Botânica/Instituto de Ciências Biológicas/Universidade Federal de Minas Gerais - UFMG; Juliana C. Silva, Programa de pós-graduação em Bioinformática/Universidade Tecnológica Federal do Paraná; Suzana T. Ivamoto, Departamento de Botânica/Instituto de Biociências de Rio Claro/Universidade Estadual Paulista; Viviane Y. Baba, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR; Silvia G. H. Souza, Laboratório de Biologia Molecular/Universidade Paranaense; Alan P. R. Lorenzetti, Programa de Pós-graduação em Genética e Biologia Molecular/Universidade Estadual de Londrina - UEL; Alexandre R. Paschoal, Programa de pós-graduação em Bioinformática/Universidade Tecnológica Federal do Paraná; Anderson R. Meda, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR; Milton Y. Nishiyama Júnior, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan; Úrsula C. de Oliveira, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan; João B. Mokochinski, Departamento de Biologia Vegetal, Instituto de Biologia/Universidade Estadual de Campinas; Romain Guyot, IRD, UMR IPME, COFFEEADAPT; Inácio L. M. Junqueira-de-Azevedo, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan; Antônio V. O. Figueira, Centro de Energia Nuclear na Agricultura/Universidade de São Paulo - USP; Paulo Mazzafera, Programa de Pós-graduação em Genética e Biologia Molecular/Universidade Estadual de Londrina - UEL; Osvaldo R. Júnior, Life Sciences Core Facility - LaCTAD/Universidade Estadual de Campinas; Luiz G. E. Vieira, Universidade do Oeste Paulista; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; Douglas S. Domingues, Departamento de Botânica/Instituto de Biociências de Rio Claro/Universidade Estadual Paulista. |
|
Título: |
An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Functional & Integrative Genomics, v. 19, n. 1, p. 151-169, January, 2019 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Coffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots.We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots. |
|
Palavras-Chave: |
Differential gene expression; Nitrogen transport; RNA-seq. |
|
Thesaurus Nal: |
MicroRNA. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196962/1/An-integrated-analysis-of-mRNA-transcriptional.pdf
|
|
Marc: |
LEADER 02379naa a2200409 a 4500
001 2108713
005 2019-05-06
008 2019 bl uuuu u00u1 u #d
100 1 $aSANTOS, T. B. dos
245 $aAn integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots$binsights on nitrogen starvation responses.$h[electronic resource]
260 $c2019
520 $aCoffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots.We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots.
650 $aMicroRNA
653 $aDifferential gene expression
653 $aNitrogen transport
653 $aRNA-seq
700 1 $aSOARES, J. D. M.
700 1 $aLIMA, J. E.
700 1 $aSILVA, J. C.
700 1 $aIVAMOTO, S. T.
700 1 $aBABA, V. Y.
700 1 $aSOUZA, S. G. H.
700 1 $aLORENZETTI, A. P. R.
700 1 $aPASCHOAL, A. R.
700 1 $aMEDA, A. R.
700 1 $aNISHIYAMA JÚNIOR, M. Y.
700 1 $aOLIVEIRA, U. C. de
700 1 $aMOKOCHINSKI, J. B.
700 1 $aGUYOT, R.
700 1 $aJUNQUEIRA-DE-AZEVEDO, I. L. M.
700 1 $aFIGUEIR, A. V. O.
700 1 $aMAZZAFERA, P.
700 1 $aR. JÚNIO, O.
700 1 $aVIEIRA, L. G. E.
700 1 $aPEREIRA, L. F. P.
700 1 $aDOMINGUES, D. S.
773 $tFunctional & Integrative Genomics$gv. 19, n. 1, p. 151-169, January, 2019
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Café (CNPCa) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
30/03/2009 |
|
Data da última atualização: |
30/06/2023 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. |
|
Afiliação: |
Elliot Watanabe Kitajima, ESALQ; R. F. Calegario, ESALQ; Valdenice Moreira Novelli, APTA; Eliane Cristina Locali-Fabris, APTA; Marinês Bastianel, APTA; F. Francischini, Alellyx Genomics; Juliana Freitas-Ástua, CNPMF. |
|
Título: |
In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. |
|
Descrição Física: |
1 CD ROM |
|
Idioma: |
Inglês |
|
Conteúdo: |
Among the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurred between membranes of adjacent cells near the midgut and prosomal gland. Anti-NC antibodies specifically labeled these particles both in the plant and in the mite thus confirming their viral nature. Viroplasmas, present in infected plant cells, are also immunolabeled by anti-NC antibody, but they were not found in the mite tissues. A possible mechanism to explain the viral presence between cells, similar to diapedesis of the leucocytes in blood vessels, is being suggested. These cytological evidences in the mite reinforce the concept that the CiLV-C/Brevipalpus relation is of circulative rather than replicative type. This information is relevant for epidemiology and control strategies. MenosAmong the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurr... Mostrar Tudo |
|
Thesagro: |
Ácaro; Vírus. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03084nam a2200217 a 4500
001 1655634
005 2023-06-30
008 2008 bl uuuu u00u1 u #d
100 1 $aKITAJIMA, E. W.
245 $aIn situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type.$h[electronic resource]
260 $aIn: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.]$c2008
300 $c1 CD ROM
520 $aAmong the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurred between membranes of adjacent cells near the midgut and prosomal gland. Anti-NC antibodies specifically labeled these particles both in the plant and in the mite thus confirming their viral nature. Viroplasmas, present in infected plant cells, are also immunolabeled by anti-NC antibody, but they were not found in the mite tissues. A possible mechanism to explain the viral presence between cells, similar to diapedesis of the leucocytes in blood vessels, is being suggested. These cytological evidences in the mite reinforce the concept that the CiLV-C/Brevipalpus relation is of circulative rather than replicative type. This information is relevant for epidemiology and control strategies.
650 $aÁcaro
650 $aVírus
700 1 $aCALEGARIO, R. F.
700 1 $aNOVELLI, V. M.
700 1 $aLOCALI-FABRIS, E. C.
700 1 $aBASTIANEL, M.
700 1 $aFRANCISCHINI, F.
700 1 $aFREITAS-ASTUA, J.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
