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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
08/10/2008 |
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Data da última atualização: |
08/10/2008 |
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Autoria: |
AMORIM, M. R.; LIMA, T. C. A.; SILVA, G. R.; ALMEIDA, G. M.; VIEIRA NETO, J. M.; PEREIRA, F. de M.; LIMA, P. S. da C.; BRITO, F. B.; LOPES, M. T. do R.; CAMARGO, R. C.; DINIZ, F. M. |
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Título: |
Extração não letal de DNA em abelhas do gênero Melipona. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Teresina: Embrapa Meio-Norte, 2007. |
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Páginas: |
6 p. |
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Série: |
(Embrapa Meio-Norte. Comunicado técnico, 199). |
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Idioma: |
Português |
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Conteúdo: |
O propósito deste trabalho foi identificar a viabilidade de protocolos de extração não letal de DNA em abelhas Melipona, utilizando-se o tarso e a extremidade da asa, e verificar a qualidade do material extraído para reações de polimerase em cadeia (PCR), especificamente uma região do DNA mitocondrial frequentemente utilizada em estudos de evolução molecular. |
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Categoria do assunto: |
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Marc: |
LEADER 01072nam a2200253 a 4500
001 1125113
005 2008-10-08
008 2007 bl uuuu u0uu1 u #d
100 1 $aAMORIM, M. R.
245 $aExtração não letal de DNA em abelhas do gênero Melipona.
260 $aTeresina: Embrapa Meio-Norte$c2007
300 $a6 p.
490 $a(Embrapa Meio-Norte. Comunicado técnico, 199).
520 $aO propósito deste trabalho foi identificar a viabilidade de protocolos de extração não letal de DNA em abelhas Melipona, utilizando-se o tarso e a extremidade da asa, e verificar a qualidade do material extraído para reações de polimerase em cadeia (PCR), especificamente uma região do DNA mitocondrial frequentemente utilizada em estudos de evolução molecular.
700 1 $aLIMA, T. C. A.
700 1 $aSILVA, G. R.
700 1 $aALMEIDA, G. M.
700 1 $aVIEIRA NETO, J. M.
700 1 $aPEREIRA, F. de M.
700 1 $aLIMA, P. S. da C.
700 1 $aBRITO, F. B.
700 1 $aLOPES, M. T. do R.
700 1 $aCAMARGO, R. C.
700 1 $aDINIZ, F. M.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
DORNELES, E. M. S.; FONSECA, M. D. A. M.; ABREU, J. A. P.; LAGE, A. P.; BRITO, M. A. V. P.; PEREIRA, C. R.; BRANDAO, H. de M.; GUIMARÃES, A. S.; HEINEMANN, M. B. |
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Afiliação: |
Elaine M. S. Dorneles; Mariana D. A. M. Fonseca; Juliana A. P. Abreu; Andrey P. Lage; Maria A. V. P. Brito; Carine R. Pereira; HUMBERTO DE MELLO BRANDAO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; Marcos B. Heinemann. |
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Título: |
Genetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase-negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Microbiology Open, v. 8, n. 5, article e736, 2019. |
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DOI: |
https://doi.org/10.1002/mbo3.736 |
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Idioma: |
Inglês |
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Conteúdo: |
The aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates? susceptibility profiles and pulsed‐field gel electrophoresis (PFGE) genotypes. Seventy‐nine isolates were used, including S. aureus (n = 71) and coagulase‐negative staphylococci (CoNS) (n = 8). Susceptibility to 12 antimicrobial agents was performed. All Staphylococcus spp. were subjected to PFGE. Staphylococcus aureus and CoNS isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among Staphylococcus spp. were observed to penicillins, folate pathway inhibitors, and tetracyclines. Twelve S. aureus and four CoNS were classified as multidrug resistance strains. Percentage of MRSA was also higher among CoNS (75%), compared to S. aureus isolates (2.81%). Adopting 100% of similarity, 34 different genotypes were identified. Association of minimum‐spanning tree (MST) analysis with data from municipalities, herds, methicillin‐resistant S. aureus (MRSA), and resistance patterns for all isolates did not show any clustering. However, a clustering pattern of bacterial species was observed. Results from this study indicate a high frequency of antimicrobial resistance, especially among CoNS, and a high genetic diversity among Staphylococcus spp. isolated from dairy cows with mastitis in Minas Gerais, Brazil. MenosThe aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates? susceptibility profiles and pulsed‐field gel electrophoresis (PFGE) genotypes. Seventy‐nine isolates were used, including S. aureus (n = 71) and coagulase‐negative staphylococci (CoNS) (n = 8). Susceptibility to 12 antimicrobial agents was performed. All Staphylococcus spp. were subjected to PFGE. Staphylococcus aureus and CoNS isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among Staphylococcus spp. were observed to penicillins, folate pathway inhibitors, and tetracyclines. Twelve S. aureus and four CoNS were classified as multidrug resistance strains. Percentage of MRSA was also higher among CoNS (75%), compared to S. aureus isolates (2.81%). Adopting 100% of similarity, 34 different genotypes were identified. Association of minimum‐spanning tree (MST) analysis with data from municipalities, herds, methicillin‐resistant S. aureus (MRSA), and resistance patterns for all isolates did not show any clustering. However, a clustering pattern of bacterial species was observed. Results from this study indicate a high frequency of antimicrobial resistance, especially among CoNS, and a high genetic diversity among Staphylococcus spp. isolated from dairy cows with mastitis in Mina... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial resistance; Coagulase-negative staphylococci; Methicillin-resistant S aureus; PFGE; Staphylococci. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208347/1/Dorneles-et-al-2019-MicrobiologyOpen.pdf
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Marc: |
LEADER 02488naa a2200289 a 4500
001 2118368
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/mbo3.736$2DOI
100 1 $aDORNELES, E. M. S.
245 $aGenetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase-negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil.$h[electronic resource]
260 $c2019
520 $aThe aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates? susceptibility profiles and pulsed‐field gel electrophoresis (PFGE) genotypes. Seventy‐nine isolates were used, including S. aureus (n = 71) and coagulase‐negative staphylococci (CoNS) (n = 8). Susceptibility to 12 antimicrobial agents was performed. All Staphylococcus spp. were subjected to PFGE. Staphylococcus aureus and CoNS isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among Staphylococcus spp. were observed to penicillins, folate pathway inhibitors, and tetracyclines. Twelve S. aureus and four CoNS were classified as multidrug resistance strains. Percentage of MRSA was also higher among CoNS (75%), compared to S. aureus isolates (2.81%). Adopting 100% of similarity, 34 different genotypes were identified. Association of minimum‐spanning tree (MST) analysis with data from municipalities, herds, methicillin‐resistant S. aureus (MRSA), and resistance patterns for all isolates did not show any clustering. However, a clustering pattern of bacterial species was observed. Results from this study indicate a high frequency of antimicrobial resistance, especially among CoNS, and a high genetic diversity among Staphylococcus spp. isolated from dairy cows with mastitis in Minas Gerais, Brazil.
653 $aAntimicrobial resistance
653 $aCoagulase-negative staphylococci
653 $aMethicillin-resistant S aureus
653 $aPFGE
653 $aStaphylococci
700 1 $aFONSECA, M. D. A. M.
700 1 $aABREU, J. A. P.
700 1 $aLAGE, A. P.
700 1 $aBRITO, M. A. V. P.
700 1 $aPEREIRA, C. R.
700 1 $aBRANDAO, H. de M.
700 1 $aGUIMARÃES, A. S.
700 1 $aHEINEMANN, M. B.
773 $tMicrobiology Open$gv. 8, n. 5, article e736, 2019.
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Embrapa Gado de Leite (CNPGL) |
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