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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/06/2015 |
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Data da última atualização: |
11/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, H.; LOPES, F. M.; SILLA, P. R.; HUNGRIA, M. |
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Afiliação: |
HELTON DE AZEVEDO, CNPSO; Fabricio Martins Lopes; PAULO ROBERTO SILLA, CNPSO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
A database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC Genomics, London, v. 16, 2015. Suppl 5, S10. |
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Páginas: |
11 p. |
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ISSN: |
1471-2164 |
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Idioma: |
Inglês |
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Notas: |
Edição dos Proceedings of the 10th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-Meeting 2014), Belo Horizonte, Oct. 2014. |
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Conteúdo: |
Biological nitrogen fixation, with an emphasis on the legume-rhizobia symbiosis, is a key process for agriculture and the environment, allowing the replacement of nitrogen fertilizers, reducing water pollution by nitrate as well as emission of greenhouse gases. Soils contain numerous strains belonging to the bacterial genus Bradyrhizobium, which establish symbioses with a variety of legumes. However, due to the high conservation of Bradyrhizobium 16S rRNA genes - considered as the backbone of the taxonomy of prokaryotes - few species have been delineated. The multilocus sequence analysis (MLSA) methodology, which includes analysis of housekeeping genes, has been shown to be promising and powerful for defining bacterial species, and, in this study, it was applied to Bradyrhizobium, species, increasing our understanding of the diversity of nitrogen-fixing bacteria. Description: Classification of bacteria of agronomic importance is relevant to biodiversity, as well as to biotechnological manipulation to improve agricultural productivity. We propose the construction of an online database that will provide information and tools using MLSA to improve phylogenetic and taxonomic characterization of Bradyrhizobium, allowing the comparison of genomic sequences with those of type and representative strains of each species. Conclusion: A database for the taxonomic and phylogenetic identification of the Bradyrhizobium, genus, using MLSA, will facilitate the use of biological data available through an intuitive web interface. Sequences stored in the on-line database can be compared with multiple sequences of other strains with simplicity and agility through multiple alignment algorithms and computational routines integrated into the database. The proposed database and software tools are available at http://mlsa.cnpso.embrapa.br, and can be used, free of charge, by researchers worldwide to classify Bradyrhizobium, strains; the database and software can be applied to replicate the experiments presented in this study as well as to generate new experiments. The next step will be expansion of the database to include other rhizobial species. MenosBiological nitrogen fixation, with an emphasis on the legume-rhizobia symbiosis, is a key process for agriculture and the environment, allowing the replacement of nitrogen fertilizers, reducing water pollution by nitrate as well as emission of greenhouse gases. Soils contain numerous strains belonging to the bacterial genus Bradyrhizobium, which establish symbioses with a variety of legumes. However, due to the high conservation of Bradyrhizobium 16S rRNA genes - considered as the backbone of the taxonomy of prokaryotes - few species have been delineated. The multilocus sequence analysis (MLSA) methodology, which includes analysis of housekeeping genes, has been shown to be promising and powerful for defining bacterial species, and, in this study, it was applied to Bradyrhizobium, species, increasing our understanding of the diversity of nitrogen-fixing bacteria. Description: Classification of bacteria of agronomic importance is relevant to biodiversity, as well as to biotechnological manipulation to improve agricultural productivity. We propose the construction of an online database that will provide information and tools using MLSA to improve phylogenetic and taxonomic characterization of Bradyrhizobium, allowing the comparison of genomic sequences with those of type and representative strains of each species. Conclusion: A database for the taxonomic and phylogenetic identification of the Bradyrhizobium, genus, using MLSA, will facilitate the use of biological data availab... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/125643/1/A-database-for-the-taxonomic-and-phylogenetic.pdf
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Marc: |
LEADER 02982naa a2200217 a 4500
001 2018160
005 2017-05-11
008 2015 bl uuuu u00u1 u #d
022 $a1471-2164
100 1 $aAZEVEDO, H.
245 $aA database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis.$h[electronic resource]
260 $c2015
300 $a11 p.
500 $aEdição dos Proceedings of the 10th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-Meeting 2014), Belo Horizonte, Oct. 2014.
520 $aBiological nitrogen fixation, with an emphasis on the legume-rhizobia symbiosis, is a key process for agriculture and the environment, allowing the replacement of nitrogen fertilizers, reducing water pollution by nitrate as well as emission of greenhouse gases. Soils contain numerous strains belonging to the bacterial genus Bradyrhizobium, which establish symbioses with a variety of legumes. However, due to the high conservation of Bradyrhizobium 16S rRNA genes - considered as the backbone of the taxonomy of prokaryotes - few species have been delineated. The multilocus sequence analysis (MLSA) methodology, which includes analysis of housekeeping genes, has been shown to be promising and powerful for defining bacterial species, and, in this study, it was applied to Bradyrhizobium, species, increasing our understanding of the diversity of nitrogen-fixing bacteria. Description: Classification of bacteria of agronomic importance is relevant to biodiversity, as well as to biotechnological manipulation to improve agricultural productivity. We propose the construction of an online database that will provide information and tools using MLSA to improve phylogenetic and taxonomic characterization of Bradyrhizobium, allowing the comparison of genomic sequences with those of type and representative strains of each species. Conclusion: A database for the taxonomic and phylogenetic identification of the Bradyrhizobium, genus, using MLSA, will facilitate the use of biological data available through an intuitive web interface. Sequences stored in the on-line database can be compared with multiple sequences of other strains with simplicity and agility through multiple alignment algorithms and computational routines integrated into the database. The proposed database and software tools are available at http://mlsa.cnpso.embrapa.br, and can be used, free of charge, by researchers worldwide to classify Bradyrhizobium, strains; the database and software can be applied to replicate the experiments presented in this study as well as to generate new experiments. The next step will be expansion of the database to include other rhizobial species.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aLOPES, F. M.
700 1 $aSILLA, P. R.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics, London$gv. 16, 2015. Suppl 5, S10.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
01/12/1999 |
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Data da última atualização: |
01/12/1999 |
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Autoria: |
EUCLIDES FILHO, K.; FIGUEIREDO, G. R. de; EUCLIDES, V. P. B.; SILVA, L. O. C. da; CUSINATO, V. Q. |
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Afiliação: |
EMBRAPA. Centro Nacional de Pesquisa de Gado de Corte (Campo Grande, MS). |
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Título: |
Desempenho de animais Nelore e seus mesticos com Caracu, Angus e Simental. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 36., 1999, Porto Alegre. Resumos dos trabalhos apresentados. Porto Alegre: SBZ, 1999. |
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Páginas: |
p.146. |
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Idioma: |
Português |
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Notas: |
CNPGC. |
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Palavras-Chave: |
Bovino de corte; Caracu; Desempenho; Eficiencia; Nelore; Productivity; Simental. |
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Thesagro: |
Cruzamento; Ganho de Peso. |
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Thesaurus NAL: |
Angus; beef cattle; crossbreds; Nellore; Simmental; weight gain. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01010naa a2200361 a 4500
001 1322149
005 1999-12-01
008 1999 bl uuuu u00u1 u #d
100 1 $aEUCLIDES FILHO, K.
245 $aDesempenho de animais Nelore e seus mesticos com Caracu, Angus e Simental.
260 $c1999
300 $ap.146.
500 $aCNPGC.
650 $aAngus
650 $abeef cattle
650 $acrossbreds
650 $aNellore
650 $aSimmental
650 $aweight gain
650 $aCruzamento
650 $aGanho de Peso
653 $aBovino de corte
653 $aCaracu
653 $aDesempenho
653 $aEficiencia
653 $aNelore
653 $aProductivity
653 $aSimental
700 1 $aFIGUEIREDO, G. R. de
700 1 $aEUCLIDES, V. P. B.
700 1 $aSILVA, L. O. C. da
700 1 $aCUSINATO, V. Q.
773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 36., 1999, Porto Alegre. Resumos dos trabalhos apresentados. Porto Alegre: SBZ, 1999.
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