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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
20/06/2018 |
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Data da última atualização: |
20/06/2018 |
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Autoria: |
LOPES, E. S.; MACIEL, W. C.; MEDEIROS, P. H. Q. S.; BONA, M. D.; BINDÁ, A. H.; LIMA, S. V. G.; GAIO, F. C.; TEIXEIRA, R. S. C. |
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Afiliação: |
Elisângela S. Lopes, Programa de Pós-Graduação em Ciências Veterinárias, PPGCV/Labeo Faculdade de Veterinária/Universidade Estadual do Ceará - UECE; William C. Maciel, Programa de Pós-Graduação em Ciências Veterinárias, PPGCV/Labeo Faculdade de Veterinária/Universidade Estadual do Ceará - UECE; Pedro Henrique Q. S. Medeiros, Laboratório de Toxinologia Molecular/Escola de Medicina/Universidade Federal do Ceará - UFC; Mariana D. Bona, Laboratório de Toxinologia Molecular/Escola de Medicina/Universidade Federal do Ceará - UFC; Alexandre H. Bindá, Laboratório de Toxinologia Molecular/Escola de Medicina/Universidade Federal do Ceará - UFC; Suzan V. G. Lima, Programa de Pós-Graduação em Ciências Veterinárias, PPGCV/Labeo Faculdade de Veterinária/Universidade Estadual do Ceará - UECE; Fernanda C. Gaio, Programa de Pós-Graduação em Ciências Veterinárias, PPGCV/Labeo Faculdade de Veterinária/Universidade Estadual do Ceará - UECE; Régis S. C. Teixeira, Programa de Pós-Graduação em Ciências Veterinárias, PPGCV/Labeo Faculdade de Veterinária/Universidade Estadual do Ceará - UECE. |
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Título: |
Molecular diagnosis of diarrheagenic Escherichia coli isolated from Psittaciformes of illegal wildlife trade. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 762-766, abril 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Diagnóstico molecular de Escherichia coli diarreiogênicas isoladas de Psittaciformes do tráfico ilegal de animais silvestres. |
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Conteúdo: |
Diarrheagenic Escherichia coli (DEC) are considered one of the major causes of human diarrhea in developing countries. Some studies have pointed wild birds as important reservoirs for these pathogens. However, scarce species from the Psittaciformes order have been investigated. This study aimed to evaluate the presence of DEC strains in Psittaciformes from illegal wildlife trade. A total of 78 E. coli strains isolated from cloacal swab samples of 167 Psittaciformes in the Ceará State, Brazil, were evaluated regarding the presence of the following DEC virulence genes by polymerase chain reaction (PCR): eaeA and bfpA genes (Enteropathogenic E. coli ? EPEC); stx1 and stx2 (Shiga toxin-producing E. coli - STEC); estA and eltB (Enterotoxigenic E. coli - ETEC); ipaH (Enteroinvasive E. coli - EIEC); aatA and aaiC (Enteroaggregative E. coli - EAEC). Positive strains for eaeA and bfpA genes were considered typical EPEC, while strain positive exclusively for the eaeA gene were classified as atypical EPEC. The eaeA gene was identified in 20 E. coli strains and bfpA in 22 isolates. In addition, 11 and 9 belonged to tEPEC and aEPEC, respectively. No strain was positive for stx1 or stx2. A total of 47 (60.3%) strains and a total of 136 birds (81.4%) were negative for the remaining DEC pathotypes investigated. In conclusion, psittacine from illegal wildlife trade in Ceará State, Brazil, presented a relevant prevalence of typical and atypical EPEC, potentially playing a role as reservoirs of DEC strains in the environment. Thus, proper control measures must be adopted to block the spread of these pathogens. MenosDiarrheagenic Escherichia coli (DEC) are considered one of the major causes of human diarrhea in developing countries. Some studies have pointed wild birds as important reservoirs for these pathogens. However, scarce species from the Psittaciformes order have been investigated. This study aimed to evaluate the presence of DEC strains in Psittaciformes from illegal wildlife trade. A total of 78 E. coli strains isolated from cloacal swab samples of 167 Psittaciformes in the Ceará State, Brazil, were evaluated regarding the presence of the following DEC virulence genes by polymerase chain reaction (PCR): eaeA and bfpA genes (Enteropathogenic E. coli ? EPEC); stx1 and stx2 (Shiga toxin-producing E. coli - STEC); estA and eltB (Enterotoxigenic E. coli - ETEC); ipaH (Enteroinvasive E. coli - EIEC); aatA and aaiC (Enteroaggregative E. coli - EAEC). Positive strains for eaeA and bfpA genes were considered typical EPEC, while strain positive exclusively for the eaeA gene were classified as atypical EPEC. The eaeA gene was identified in 20 E. coli strains and bfpA in 22 isolates. In addition, 11 and 9 belonged to tEPEC and aEPEC, respectively. No strain was positive for stx1 or stx2. A total of 47 (60.3%) strains and a total of 136 birds (81.4%) were negative for the remaining DEC pathotypes investigated. In conclusion, psittacine from illegal wildlife trade in Ceará State, Brazil, presented a relevant prevalence of typical and atypical EPEC, potentially playing a role as reservoirs o... Mostrar Tudo |
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Palavras-Chave: |
EPEC; Tráfico de animal silvestre. |
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Thesagro: |
Bacteriose; Escherichia Coli. |
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Thesaurus Nal: |
Birds; Psittaciformes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178817/1/Molecular-diagnosis-of-diarrheagenic-Escherichia-coli.pdf
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Marc: |
LEADER 02627naa a2200289 a 4500
001 2092628
005 2018-06-20
008 2018 bl uuuu u00u1 u #d
100 1 $aLOPES, E. S.
245 $aMolecular diagnosis of diarrheagenic Escherichia coli isolated from Psittaciformes of illegal wildlife trade.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Diagnóstico molecular de Escherichia coli diarreiogênicas isoladas de Psittaciformes do tráfico ilegal de animais silvestres.
520 $aDiarrheagenic Escherichia coli (DEC) are considered one of the major causes of human diarrhea in developing countries. Some studies have pointed wild birds as important reservoirs for these pathogens. However, scarce species from the Psittaciformes order have been investigated. This study aimed to evaluate the presence of DEC strains in Psittaciformes from illegal wildlife trade. A total of 78 E. coli strains isolated from cloacal swab samples of 167 Psittaciformes in the Ceará State, Brazil, were evaluated regarding the presence of the following DEC virulence genes by polymerase chain reaction (PCR): eaeA and bfpA genes (Enteropathogenic E. coli ? EPEC); stx1 and stx2 (Shiga toxin-producing E. coli - STEC); estA and eltB (Enterotoxigenic E. coli - ETEC); ipaH (Enteroinvasive E. coli - EIEC); aatA and aaiC (Enteroaggregative E. coli - EAEC). Positive strains for eaeA and bfpA genes were considered typical EPEC, while strain positive exclusively for the eaeA gene were classified as atypical EPEC. The eaeA gene was identified in 20 E. coli strains and bfpA in 22 isolates. In addition, 11 and 9 belonged to tEPEC and aEPEC, respectively. No strain was positive for stx1 or stx2. A total of 47 (60.3%) strains and a total of 136 birds (81.4%) were negative for the remaining DEC pathotypes investigated. In conclusion, psittacine from illegal wildlife trade in Ceará State, Brazil, presented a relevant prevalence of typical and atypical EPEC, potentially playing a role as reservoirs of DEC strains in the environment. Thus, proper control measures must be adopted to block the spread of these pathogens.
650 $aBirds
650 $aPsittaciformes
650 $aBacteriose
650 $aEscherichia Coli
653 $aEPEC
653 $aTráfico de animal silvestre
700 1 $aMACIEL, W. C.
700 1 $aMEDEIROS, P. H. Q. S.
700 1 $aBONA, M. D.
700 1 $aBINDÁ, A. H.
700 1 $aLIMA, S. V. G.
700 1 $aGAIO, F. C.
700 1 $aTEIXEIRA, R. S. C.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 4, p. 762-766, abril 2018
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Embrapa Unidades Centrais (AI-SEDE) |
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| 4. |  | BERNARDI, A. C. de C. Potássio em solos sob pastagem intensiva. In: APOIO AO USO BALANCEADO DE POTÁSSIO NA AGRICULTURA BRASILEIRA, 2013, Piracicaba. 10 anos de parceria IPI e Embrapa - Resumos expandidos. Piracicaba: IPI, 2013. p. 46-47| Tipo: Artigo em Anais de Congresso |
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| 13. | | BERNARDI, A. C. C.; BARIONI-JUNIOR, W.; INAMASU, R. Y. Análise de correspondência múltipla para caracterização do perfil de adotantes de agricultura de precisão no Brasil. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 73-76. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação; Embrapa Pecuária Sudeste. |
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| 20. | | INAMASU, R. Y.; BERNARDI, A. C. de C. Agricultura de precisão. In: BERNARDI, A. C. de C.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; INAMASU, R. Y. (Ed.). Agricultura de precisão: resultados de um novo olhar. Brasília, DF: Embrapa, 2014. p. 21-33.| Tipo: Capítulo em Livro Técnico-Científico |
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