Data/hora: 09/06/2024 02:51:24

Biblioteca(s): 	Embrapa Arroz e Feijão; Embrapa Meio Ambiente.	
Data corrente: 	13/09/2022	
Data da última atualização: 	14/09/2022	
Autoria: 	LOCATELLI, G. O.; PIMENTEL, M. F.; BUENO, L. A.; LOBO JUNIOR, M.; MASCARIN, G. M.; FINKLER, C. L. L.	
Afiliação: 	GABRIEL OLIVO LOCATELLI, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; MARIA FERNANDA PIMENTEL, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LUCIANO AVALLONE BUENO, UNIVERSIDADE FEDERAL DO ABC; MURILLO LOBO JUNIOR, CNPAF; GABRIEL MOURA MASCARIN, CNPMA; CHRISTINE LAMENHA LUNA FINKLER, UNIVERSIDADE FEDERAL DE PERNAMBUCO.	
Título: 	Production of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Biocatalysis and Agricultural Biotechnology, v. 44, article 102455, 2022.	
ISSN: 	1878-8181	
DOI: 	https://doi.org/10.1016/j.bcab.2022.102455	
Idioma: 	Inglês	
Conteúdo: 	Abstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development.	
Palavras-Chave: 	Autolyzed yeast; Biofungicides; C N ratio; Microsclerotia.	
Thesagro: 	Carbono; Farinha; Fermentação; Fungo Para Controle Biológico; Nitrogênio; Sorgo; Trichoderma.	
Thesaurus Nal: 	Biological control agents; Liquid state fermentation; Sorghum flour; Trichoderma asperellum.	
Categoria do assunto: 	H Saúde e Patologia
X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPMA - (17231-1) - UPC - AP - DD -  -  -  - DIS
CNPAF - (36504-1) - UPC - AP - DD - 2022 -  - 2022 - DIS