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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
20/06/1996 |
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Data da última atualização: |
20/06/1996 |
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Autoria: |
GONCALVES, V. A.; LOBATO, E. J. V.; COUTO, D.; SILVA, S. M. da. |
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Título: |
Determinacao da tendencia anual e espacial da disponibilidade hidrica no perfil do solo em diferentes condicoes climaticas. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
IN: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 9. Campina Grande, PB, 1995. Anais. Campina Grande: SBA, 1995. p.168-169 |
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Idioma: |
Português |
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Conteúdo: |
O levantamento espacial e periodico da amplitide da dificiencia e do excesso hidrico no perfil do solo e ferramenta importante em estudos eco-climaticos. Foi montado um modelo matematico combinado, que permite, via software, e conhecimento da tendencia anual da evolucao da disponibilidade hidrica do solo, em diferentes condicoes climaticas, a partir dos dados termo-pluviometricos de 249 localidades distribuidas na Regiao Centro-Oeste e demais regioes geograficas brasileiras (NORMAIS CLIMATOLOGICAS, 1992). O modelo combinado, acessivel ao computador atraves de programas executaveis em linguagem Pascal, fornece valores do escedente e da deficiencia hidricos estreitamente correlacionados com aqueles obtidos pelo metode tabular tradicional. Outrossim, alem das informacoes correlatas ao balanco hidrico, o aplicativo permite a obtencao da formula climatica local, associada a informacoes complementares relacionadas ao mesoclima. |
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Palavras-Chave: |
Ecoclimaticos; Hidricos; Pluviometricos. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01581naa a2200193 a 4500
001 1364043
005 1996-06-20
008 1995 bl uuuu u00u1 u #d
100 1 $aGONCALVES, V. A.
245 $aDeterminacao da tendencia anual e espacial da disponibilidade hidrica no perfil do solo em diferentes condicoes climaticas.
260 $c1995
520 $aO levantamento espacial e periodico da amplitide da dificiencia e do excesso hidrico no perfil do solo e ferramenta importante em estudos eco-climaticos. Foi montado um modelo matematico combinado, que permite, via software, e conhecimento da tendencia anual da evolucao da disponibilidade hidrica do solo, em diferentes condicoes climaticas, a partir dos dados termo-pluviometricos de 249 localidades distribuidas na Regiao Centro-Oeste e demais regioes geograficas brasileiras (NORMAIS CLIMATOLOGICAS, 1992). O modelo combinado, acessivel ao computador atraves de programas executaveis em linguagem Pascal, fornece valores do escedente e da deficiencia hidricos estreitamente correlacionados com aqueles obtidos pelo metode tabular tradicional. Outrossim, alem das informacoes correlatas ao balanco hidrico, o aplicativo permite a obtencao da formula climatica local, associada a informacoes complementares relacionadas ao mesoclima.
653 $aEcoclimaticos
653 $aHidricos
653 $aPluviometricos
700 1 $aLOBATO, E. J. V.
700 1 $aCOUTO, D.
700 1 $aSILVA, S. M. da
773 $tIN: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 9. Campina Grande, PB, 1995. Anais. Campina Grande: SBA, 1995. p.168-169
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Embrapa Tabuleiros Costeiros (CPATC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
29/11/2018 |
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Data da última atualização: |
30/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
OKINO, C. H.; GIGLIOTI, R.; Silva, P. C.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; Rodrigo Giglioti, UNESP; Pamella Cristini Silva, UNICEP; Henrique Nunes de Oliveira, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Comparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 6, p. 2671-2680, 2018. |
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DOI: |
10.1007/s11033-018-4436-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydrolysis probe here developed presented higher sensitivity compared to intercalating dye system. This study has contributed to the improvement of molecular diagnosis of bovine babesiosis, which may improve epidemiological studies related to these pathogens. MenosBovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydro... Mostrar Tudo |
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Palavras-Chave: |
DNA extraction; Hydrolysis probe; Matrix sample; QPCR. |
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Thesagro: |
Babesia Bovis. |
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Thesaurus NAL: |
Babesiosis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02517naa a2200253 a 4500
001 2100307
005 2018-11-30
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-018-4436-9$2DOI
100 1 $aOKINO, C. H.
245 $aComparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring.$h[electronic resource]
260 $c2018
520 $aBovine babesiosis caused by protozoan parasites Babesia bovis and B. bigemina is one of the most important causes of losses for the livestock industry in tropical and subtropical regions of the world. Therefore, highly sensitive and specific tools for these hemoparasites detection and monitoring are required, especially in carrier animals, in which low parasite levels were usually present. In this context, qPCR assays have been successfully and fairly used in last years. Aiming to improve the performance of Babesia levels monitoring by qPCR, some of main aspects of this methodology that may influence results were tested: DNA extraction kits, whole blood EDTA pre-treatment, blood source (tip of tail or jugular vein), erythrocytes isolation, FTA card interference and qPCR system of detection. Under our experimental conditions, both EDTA pre-treatment and FTA card application have no influence on the sensitivity of detection, and two DNA extraction kits provided higher sensitivity compared to others. As expected, blood samples collected from the tip of tail vessels presented higher levels of B. bovis DNA compared to those obtained from the jugular vein, and erythrocytes processed isolated has also improved the sensitivity compared to whole blood. Moreover, both qPCR assays here developed using hydrolysis probes for B. bovis and B. bigemina detection, presented enhanced reproducibility compared to qPCR assays using intercalating dye system. Even, qPCR for B. bigemina using hydrolysis probe here developed presented higher sensitivity compared to intercalating dye system. This study has contributed to the improvement of molecular diagnosis of bovine babesiosis, which may improve epidemiological studies related to these pathogens.
650 $aBabesiosis
650 $aBabesia Bovis
653 $aDNA extraction
653 $aHydrolysis probe
653 $aMatrix sample
653 $aQPCR
700 1 $aGIGLIOTI, R.
700 1 $aSilva, P. C.
700 1 $aOLIVEIRA, H. N. de
700 1 $aOLIVEIRA, M. C. de S.
773 $tMolecular Biology Reports$gv. 45, n. 6, p. 2671-2680, 2018.
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