Data/hora: 05/06/2024 03:43:14

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 25/10/2001
Data da última atualização: | 17/06/2019
Autoria: | LLOYD, G.; McCOWN, B.
Título: | Commercially feasible micropropagation of mountain laurel, kalmia latifolia, by use of shoot-tip culture.
Ano de publicação: | 1986
Fonte/Imprenta: | Proceedings of International Plant Propagation Society, v. 30, p. 421-427, 1986.
Idioma: | Inglês
Conteúdo: | The multiplication at rates feasible for commercial production of mountain laurel. Kalmia latifalia. by micropropagation using shoot-tip cultures has been demonstrated. Shoot-tip explants placed initially in liquid woody plant medium (WPM) supplemented with 4-16 !iM N6_(~2-isopentenyl)-adenine (2iP) produced axillary shoots by 1 to 2 months. These new shoots were excised from the original explant and placed on the same WPM solidified with agar. The resultant shoot mass was subcultured monthly. Actively multiplying shoot-tip cultures were produced within 6 months. A comparison of 7 concentrations of 2iP, varying from Oto 64 !iMo showed that a concentration of 8 !iM 2iP produced the greatest number of utilizatble shoots after 8 weeks in culture. Stock cultures were maintained or increased monthly by removing and subculturing shoots elongating from the basal mass. Thirty to forty utilizable shoots were harvested from each culture 6 to 8 weeks after the initial subculture. Multiplication rates of 8 to 10 times were readily achieved. Harvested shoots rooted with 73% success in 4 to 6 weeks when placed in a 100% peat medium in a high humidity chamber. After a period of acclimation. these plants can be treated like young seedlings in commercial production.
Palavras-Chave: | Shoot tip culture.
Thesagro: | Meristema; Micropropagação.
Thesaurus Nal: | Kalmia latifolia; micropropagation.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (6405-1) - ADD - AP - DD -  -  -  - DIS