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Registro Completo |
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Biblioteca(s): |
Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Pecuária Sul; Embrapa Unidades Centrais. |
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Data corrente: |
08/01/1991 |
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Data da última atualização: |
25/03/2013 |
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Autoria: |
LLERAS PEREZ, E. |
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Afiliação: |
EMBRAPA. |
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Título: |
Amostragem de germoplasma, domesticação e definição de áreas de alta diversidade de espécies perenes tropicais e subtropicais: Consultant final report IICA/EMBRAPA-PROCENSUL II. |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Brasília, DF: IICA: EMBRAPA, 1989. |
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Páginas: |
49 p. |
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Série: |
(IICA. Publicações miscelaneas, A4/BR-89-028). |
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Idioma: |
Português |
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Conteúdo: |
O presente relatório cobre basicamente as atividades desenvolvidas pelo consultor entre 1982 a 1988. Para atende-las é dada uma explanação sobre o embasamento teórico que fundamentou às mesmas. |
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Palavras-Chave: |
Espécie perene; Especie perene subtropical; Especie perene tropical; Perennial; Perennial crop; Perennial subtropical; Plant; Project; Projeto; Subtropical; Tropical; Tropical species; Tropico. |
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Thesagro: |
Amostragem; Botânica; Cultivo Perene; Germoplasma; Pesquisa; Planta; Taxonomia. |
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Thesaurus Nal: |
botany; germplasm; research; taxonomy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01384nam a2200421 a 4500
001 1352546
005 2013-03-25
008 1989 bl uuuu u0uu1 u #d
100 1 $aLLERAS PEREZ, E.
245 $aAmostragem de germoplasma, domesticação e definição de áreas de alta diversidade de espécies perenes tropicais e subtropicais$bConsultant final report IICA/EMBRAPA-PROCENSUL II.
260 $aBrasília, DF: IICA: EMBRAPA$c1989
300 $a49 p.
490 $a(IICA. Publicações miscelaneas, A4/BR-89-028).
520 $aO presente relatório cobre basicamente as atividades desenvolvidas pelo consultor entre 1982 a 1988. Para atende-las é dada uma explanação sobre o embasamento teórico que fundamentou às mesmas.
650 $abotany
650 $agermplasm
650 $aresearch
650 $ataxonomy
650 $aAmostragem
650 $aBotânica
650 $aCultivo Perene
650 $aGermoplasma
650 $aPesquisa
650 $aPlanta
650 $aTaxonomia
653 $aEspécie perene
653 $aEspecie perene subtropical
653 $aEspecie perene tropical
653 $aPerennial
653 $aPerennial crop
653 $aPerennial subtropical
653 $aPlant
653 $aProject
653 $aProjeto
653 $aSubtropical
653 $aTropical
653 $aTropical species
653 $aTropico
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/03/2011 |
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Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ARCURI, E. F.; ÂNGELO, F. F.; TALON, R.; BORGES, M. de F.; LEROY, S.; LOISEAU, G.; LANGE, C. C.; ANDRADE, N. J. de; MONTET, D. |
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Afiliação: |
EDNA FROEDER ARCURI, CNPGL; FABÍOLA FONSECA ÂNGELO, UFV; RÉGINE TALON, Institut National de la Recherche Agronomique; MARIA DE FATIMA BORGES, CNPAT; SABINE LEROY, Institut National de la Recherche Agronomique; GÉRARD LOISEAU, Centre de Coopération Internationale en Recherche Agronomique pour le Dévelopment; CARLA CHRISTINE LANGE, CNPGL; NÉLIO JOSÉ DE ANDRADE, UFV; DIDIER MONTET, Céntre de Coopération Internationale en Recherche Agronomique pour le Dévelopment. |
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Título: |
Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Food Protection, v. 73, n. 12, p. 2225-2231, 2010. |
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DOI: |
https://doi.org/10.4315/0362-028X-73.12.2225 |
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Idioma: |
Inglês |
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Conteúdo: |
A group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety. MenosA group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high ge... Mostrar Tudo |
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Palavras-Chave: |
Minas frescal cheese. |
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Thesagro: |
Staphylococcus Aureus. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/881434/1/Toxigenic-status-of-Staphylococcus-aureus-isolated-from-bovine.pdf
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Marc: |
LEADER 02530naa a2200253 a 4500
001 1881434
005 2022-08-09
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4315/0362-028X-73.12.2225$2DOI
100 1 $aARCURI, E. F.
245 $aToxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil.$h[electronic resource]
260 $c2010
520 $aA group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety.
650 $aStaphylococcus Aureus
653 $aMinas frescal cheese
700 1 $aÂNGELO, F. F.
700 1 $aTALON, R.
700 1 $aBORGES, M. de F.
700 1 $aLEROY, S.
700 1 $aLOISEAU, G.
700 1 $aLANGE, C. C.
700 1 $aANDRADE, N. J. de
700 1 $aMONTET, D.
773 $tJournal of Food Protection$gv. 73, n. 12, p. 2225-2231, 2010.
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Embrapa Gado de Leite (CNPGL) |
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