|
|
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
29/05/2023 |
Data da última atualização: |
23/05/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOREIRA, V. J. V.; PINHEIRO, D. H.; LOURENCO, I. T.; BASSO, M. F.; LISEI-DE-SA, M. E.; SILVA, M. C. M. da; DANCHIN, E. G. J.; GUIMARAES, P. M.; GRYNBERG, P.; BRASILEIRO, A. C. M.; MACEDO, L. L. P. de; MORGANTE, C. V.; ENGLER, J. de A.; SA, M. F. G. de. |
Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, Biotechnology Center, PPGBCM, UFRGS; DANIELE HELOÍSA PINHEIRO, National Institute of Science and Technology; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology; MARIA E. LISEI-DE-SA, National Institute of Science and Technology; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ETIENNE G. J. DANCHIN, National Institute of Science and Technology; PATRICIA MESSEMBERG GUIMARAES, Cenargen; PRISCILA GRYNBERG, Cenargen; ANA CRISTINA MIRANDA BRASILEIRO, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Journal of Pest Science, v. 97, p. 411-427, 2024. |
DOI: |
https://doi.org/10.1007/s10340-023-01623-7 |
Idioma: |
Inglês |
Conteúdo: |
Meloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing. MenosMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disor... Mostrar Tudo |
Palavras-Chave: |
DsRNA; Host-induced gene silencing; Interações planta-nematóide; Nematoide das galhas; Plant-nematode interactions; Root-knot nematode. |
Thesagro: |
Meloidogyne Incognita; Nematóide; Parasito de Planta; Planta. |
Thesaurus Nal: |
Nematode biology; Nematode control; Plant parasitic nematodes. |
Categoria do assunto: |
-- O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/264479/1/In-planta-RNAi-targeting-Meloidogyne-incognita-Minc16803-gene-perturbs-nematode-parasitism-and-reduces-plant-susceptibility.pdf
|
Marc: |
LEADER 03269naa a2200445 a 4500 001 2154064 005 2024-05-23 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s10340-023-01623-7$2DOI 100 1 $aMOREIRA, V. J. V. 245 $aIn planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility.$h[electronic resource] 260 $c2024 520 $aMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing. 650 $aNematode biology 650 $aNematode control 650 $aPlant parasitic nematodes 650 $aMeloidogyne Incognita 650 $aNematóide 650 $aParasito de Planta 650 $aPlanta 653 $aDsRNA 653 $aHost-induced gene silencing 653 $aInterações planta-nematóide 653 $aNematoide das galhas 653 $aPlant-nematode interactions 653 $aRoot-knot nematode 700 1 $aPINHEIRO, D. H. 700 1 $aLOURENCO, I. T. 700 1 $aBASSO, M. F. 700 1 $aLISEI-DE-SA, M. E. 700 1 $aSILVA, M. C. M. da 700 1 $aDANCHIN, E. G. J. 700 1 $aGUIMARAES, P. M. 700 1 $aGRYNBERG, P. 700 1 $aBRASILEIRO, A. C. M. 700 1 $aMACEDO, L. L. P. de 700 1 $aMORGANTE, C. V. 700 1 $aENGLER, J. de A. 700 1 $aSA, M. F. G. de 773 $tJournal of Pest Science$gv. 97, p. 411-427, 2024.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Semiárido (CPATSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
03/12/1997 |
Data da última atualização: |
03/12/1997 |
Autoria: |
FURTADO, S. I.; LEITE, G. G.; BARCELLOS, A. O. |
Título: |
Avaliacao do consumo e digestibilidade de fenos de Stylosanthes guianensis cv. Mineirao e Andropogon gayanus cv. Planaltina em diferentes proporcoes na dieta de bovinos. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora, MG. Anais... Juiz de Fora: SBZ, 1997. |
Volume: |
v.2. |
Páginas: |
p.360-362. |
Idioma: |
Português |
Conteúdo: |
O objetivo do presente trabalho foi avaliar o consumo de materia seca (CMS), a digestibilidade aparente da materia seca (CDMS) e proteina bruta (CDPB), bem como a digestibilidade "in vitro" da materia seca (DIVMS) de diferentes proporcoes da mistura de fenos de Stylosanthes guianensis cv. Mineirao e Andropogon gayanus cv. Planaltina. Foram utilizados nove animais mesticos alojados individualmente em gaiolas e distribuidos em um delineamento experimental em blocos casualizados com tres tratamentos (T1 - Feno de Andropogon; T2 - 75% Feno de Andropogon + 25% Feno de Stylosanthes de T3-50% Feno de Andropogon + 50% Feno de Stylosanthes). Os resultados mostraram que o consumo voluntario nao diferiu (P>0,05) entre os tratamentos. Observou-se que houve uma tendencia de aumento no consumo a medida que se adicionou o estilosantes na dieta. Os coeficientes de digestibilidade aparente da materia seca e proteina bruta nos tratamentos com estilosantes foram maiores do que no T1 (P>0,05), porem nao apresentaram diferenca significativa entre eles (P>0,05). A DIVMS nao diferiu (P>0,05) entre os tratamentos. Os animais ganharam peso com o acrescimo do estilosantes, mostrando o beneficio da adicao da leguminosa a dieta dos animais. |
Palavras-Chave: |
Cultivar Mineirao; Cultivar Planaltina; Digestibilidade in vivo; Feed legumes; In vivo experimentation. |
Thesagro: |
Andropogon Gayanus; Bovino; Cerrado; Leguminosa Forrageira; Nutrição Animal; Stylosanthes Guianensis. |
Thesaurus NAL: |
animal nutrition; cattle; digestibility; varieties. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02352naa a2200349 a 4500 001 1553303 005 1997-12-03 008 1997 bl uuuu u00u1 u #d 100 1 $aFURTADO, S. I. 245 $aAvaliacao do consumo e digestibilidade de fenos de Stylosanthes guianensis cv. Mineirao e Andropogon gayanus cv. Planaltina em diferentes proporcoes na dieta de bovinos. 260 $c1997 300 $ap.360-362. v.2. 490 $vv.2. 520 $aO objetivo do presente trabalho foi avaliar o consumo de materia seca (CMS), a digestibilidade aparente da materia seca (CDMS) e proteina bruta (CDPB), bem como a digestibilidade "in vitro" da materia seca (DIVMS) de diferentes proporcoes da mistura de fenos de Stylosanthes guianensis cv. Mineirao e Andropogon gayanus cv. Planaltina. Foram utilizados nove animais mesticos alojados individualmente em gaiolas e distribuidos em um delineamento experimental em blocos casualizados com tres tratamentos (T1 - Feno de Andropogon; T2 - 75% Feno de Andropogon + 25% Feno de Stylosanthes de T3-50% Feno de Andropogon + 50% Feno de Stylosanthes). Os resultados mostraram que o consumo voluntario nao diferiu (P>0,05) entre os tratamentos. Observou-se que houve uma tendencia de aumento no consumo a medida que se adicionou o estilosantes na dieta. Os coeficientes de digestibilidade aparente da materia seca e proteina bruta nos tratamentos com estilosantes foram maiores do que no T1 (P>0,05), porem nao apresentaram diferenca significativa entre eles (P>0,05). A DIVMS nao diferiu (P>0,05) entre os tratamentos. Os animais ganharam peso com o acrescimo do estilosantes, mostrando o beneficio da adicao da leguminosa a dieta dos animais. 650 $aanimal nutrition 650 $acattle 650 $adigestibility 650 $avarieties 650 $aAndropogon Gayanus 650 $aBovino 650 $aCerrado 650 $aLeguminosa Forrageira 650 $aNutrição Animal 650 $aStylosanthes Guianensis 653 $aCultivar Mineirao 653 $aCultivar Planaltina 653 $aDigestibilidade in vivo 653 $aFeed legumes 653 $aIn vivo experimentation 700 1 $aLEITE, G. G. 700 1 $aBARCELLOS, A. O. 773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora, MG. Anais... Juiz de Fora: SBZ, 1997.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Cerrados (CPAC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|