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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
17/06/2021 |
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Data da última atualização: |
21/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BASSO, M. F.; COSTA, J. A.; RIBEIRO, T. P.; ARRAES, F. B. M.; LOURENCO, I. T.; MACEDO, A. F.; NEVES, M. R. das; NARDELI, S. M.; ARGE, L. W.; PEREZ, C. E. A.; SILVA, P. L. R.; MACEDO, L. L. P. de; LISEI-DE-SA, M. E.; AMORIM, R. M. S.; PINTO, E. R. de C.; SILVA, M. C. M. da; MORGANTE, C. V.; FLOH, E. I. S.; ALVES-FERREIRA, M.; SA, M. F. G. de. |
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Afiliação: |
MARCOS FERNANDO BASSO; JULIA ALMEIDA COSTA, UCB; THUANNE PIRES RIBEIRO, UNB; FABRICIO BARBOSA MONTEIRO ARRAES, UFRGS; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; AMANDA FERREIRA MACEDO, USP; MAYSA ROSA DAS NEVES; SARAH MUNIZ NARDELI, UFRJ; LUIS WILLIAN ARGE, UFRJ; CARLOS EDUARDO AUCIQUE PEREZ; PAOLO LUCAS RODRIGUES SILVA, UCB; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; MARIA EUGÊNIA LISEI-DE-SA, INCT PlantStress Biotech; REGINA MARIA SANTOS AMORIM; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; ENY IOCHEVET SEGAL FLOH, USP; MARCIO ALVES-FERREIRA, UFRJ; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Overexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 165, p. 80-93, 2021. |
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DOI: |
https://doi.org/10.1016/j.plaphy.2021.05.009 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa. |
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Conteúdo: |
The Coffea arabica HB12 gene (CaHB12), which encodes a transcription factor belonging to the HD-Zip I sub-family, is upregulated under drought, and its constitutive overexpression (35S:CaHB12OX) improves the Arabi-dopsis thaliana tolerance to drought and salinity stresses. Herein, we generated transgenic cotton events constitutively overexpressing the CaHB12 gene, characterized these events based on their increased tolerance to water deficit, and exploited the gene expression level from the CaHB12 network. The segregating events Ev8.29.1, Ev8.90.1, and Ev23.36.1 showed higher photosynthetic yield and higher water use efficiency under severe water deficit and permanent wilting point conditions compared to wild-type plants. Under well-irrigated conditions, these three promising transformed events showed an equivalent level of Abscisic acid (ABA) and decreased Indole-3-acetic acid (IAA) accumulation, and a higher putrescine/(spermidine +spermine) ratio in leaf tissues was found in the progenies of at least two transgenic cotton events compared to non-transgenic plants. In addition, genes that are considered as modulated in the A. thaliana 35S:CaHB12OX line were also shown to be modulated in several transgenic cotton events maintained under field capacity conditions. The upregulation of GhPP2C and GhSnRK2 in transgenic cotton events maintained under permanent wilting point conditions suggested that CaHB12 might act enhancing the ABA-dependent pathway. All these data confirmed that CaHB12 overexpression improved the tolerance to water deficit, and the transcriptional modulation of genes related to the ABA signaling pathway or downstream genes might enhance the defense responses to drought. The observed decrease in IAA levels indicates that CaHB12 overexpression can prevent leaf abscission in plants under or after stress. Thus, our findings provide new insights on CaHB12 gene and identify several promising cotton events for conducting field trials on water deficit tolerance and agronomic performance. MenosThe Coffea arabica HB12 gene (CaHB12), which encodes a transcription factor belonging to the HD-Zip I sub-family, is upregulated under drought, and its constitutive overexpression (35S:CaHB12OX) improves the Arabi-dopsis thaliana tolerance to drought and salinity stresses. Herein, we generated transgenic cotton events constitutively overexpressing the CaHB12 gene, characterized these events based on their increased tolerance to water deficit, and exploited the gene expression level from the CaHB12 network. The segregating events Ev8.29.1, Ev8.90.1, and Ev23.36.1 showed higher photosynthetic yield and higher water use efficiency under severe water deficit and permanent wilting point conditions compared to wild-type plants. Under well-irrigated conditions, these three promising transformed events showed an equivalent level of Abscisic acid (ABA) and decreased Indole-3-acetic acid (IAA) accumulation, and a higher putrescine/(spermidine +spermine) ratio in leaf tissues was found in the progenies of at least two transgenic cotton events compared to non-transgenic plants. In addition, genes that are considered as modulated in the A. thaliana 35S:CaHB12OX line were also shown to be modulated in several transgenic cotton events maintained under field capacity conditions. The upregulation of GhPP2C and GhSnRK2 in transgenic cotton events maintained under permanent wilting point conditions suggested that CaHB12 might act enhancing the ABA-dependent pathway. All these data confirmed th... Mostrar Tudo |
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Palavras-Chave: |
ABA-Dependent pathway; Homeobox gene; Tolerancia a seca; Water deficit. |
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Thesagro: |
Algodão; Gossypium Hirsutum. |
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Thesaurus Nal: |
Cotton. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 03462naa a2200457 a 4500
001 2133063
005 2021-07-21
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.plaphy.2021.05.009$2DOI
100 1 $aBASSO, M. F.
245 $aOverexpression of the CaHB12 transcription factor in cotton (Gossypium hirsutum) improves drought tolerance.$h[electronic resource]
260 $c2021
500 $aNa publicação: Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.
520 $aThe Coffea arabica HB12 gene (CaHB12), which encodes a transcription factor belonging to the HD-Zip I sub-family, is upregulated under drought, and its constitutive overexpression (35S:CaHB12OX) improves the Arabi-dopsis thaliana tolerance to drought and salinity stresses. Herein, we generated transgenic cotton events constitutively overexpressing the CaHB12 gene, characterized these events based on their increased tolerance to water deficit, and exploited the gene expression level from the CaHB12 network. The segregating events Ev8.29.1, Ev8.90.1, and Ev23.36.1 showed higher photosynthetic yield and higher water use efficiency under severe water deficit and permanent wilting point conditions compared to wild-type plants. Under well-irrigated conditions, these three promising transformed events showed an equivalent level of Abscisic acid (ABA) and decreased Indole-3-acetic acid (IAA) accumulation, and a higher putrescine/(spermidine +spermine) ratio in leaf tissues was found in the progenies of at least two transgenic cotton events compared to non-transgenic plants. In addition, genes that are considered as modulated in the A. thaliana 35S:CaHB12OX line were also shown to be modulated in several transgenic cotton events maintained under field capacity conditions. The upregulation of GhPP2C and GhSnRK2 in transgenic cotton events maintained under permanent wilting point conditions suggested that CaHB12 might act enhancing the ABA-dependent pathway. All these data confirmed that CaHB12 overexpression improved the tolerance to water deficit, and the transcriptional modulation of genes related to the ABA signaling pathway or downstream genes might enhance the defense responses to drought. The observed decrease in IAA levels indicates that CaHB12 overexpression can prevent leaf abscission in plants under or after stress. Thus, our findings provide new insights on CaHB12 gene and identify several promising cotton events for conducting field trials on water deficit tolerance and agronomic performance.
650 $aCotton
650 $aAlgodão
650 $aGossypium Hirsutum
653 $aABA-Dependent pathway
653 $aHomeobox gene
653 $aTolerancia a seca
653 $aWater deficit
700 1 $aCOSTA, J. A.
700 1 $aRIBEIRO, T. P.
700 1 $aARRAES, F. B. M.
700 1 $aLOURENCO, I. T.
700 1 $aMACEDO, A. F.
700 1 $aNEVES, M. R. das
700 1 $aNARDELI, S. M.
700 1 $aARGE, L. W.
700 1 $aPEREZ, C. E. A.
700 1 $aSILVA, P. L. R.
700 1 $aMACEDO, L. L. P. de
700 1 $aLISEI-DE-SA, M. E.
700 1 $aAMORIM, R. M. S.
700 1 $aPINTO, E. R. de C.
700 1 $aSILVA, M. C. M. da
700 1 $aMORGANTE, C. V.
700 1 $aFLOH, E. I. S.
700 1 $aALVES-FERREIRA, M.
700 1 $aSA, M. F. G. de
773 $tPlant Physiology and Biochemistry$gv. 165, p. 80-93, 2021.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas; Embrapa Uva e Vinho. |
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Data corrente: |
15/12/2011 |
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Data da última atualização: |
28/05/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
OLIVEIRA, P. R. D. de; RITSCHEL, P. S.; RUFATO, A. De R.; FIORAVANÇO, J. C.; FAORO, I. D.; LEITE, G. B.; DUTRA, L. F.; MAYER, N. A.; DEGENHARDT-GOLDBACH, J. |
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Afiliação: |
PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; ANDREA DE ROSSI RUFATO, CNPUV; JOAO CAETANO FIORAVANCO, CNPUV; I. D. FAORO, EPAGRI; G. B. LEITE, EPAGRI; LEONARDO FERREIRA DUTRA, CPACT; NEWTON ALEX MAYER, CPACT; JULIANA DEGENHARDT GOLD. |
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Título: |
The Brazilian pear breeding program. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Acta Horticulturae, The Hague, n. 909, p. 145-151, out. 2011. |
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ISBN: |
978-90-66055-04-9 |
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Idioma: |
Inglês |
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Notas: |
Trabalho apresentado no 11º International Pear Symposium. |
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Conteúdo: |
Pear production in Brazil reached 17,391 tons in 2008, enough to provide for a mere 20% of the local market. The lack of adapted cultivars is a major obstacle to self-sustainable pear production in the country. In 2006, a novel nationwide pear breeding program was initiated, encompassing several research institutions and aiming to develop cultivars adapted to local growth conditions in an attempt to reverse the current pear importer status of Brazil. The program is structured in distinct research lines, including: enlargement of the genetic variation available in germplasm collections; formation and evaluation of segregating populations, hybrids and selections of scion and rootstock materials; network cultivar agricultural evaluations; screening of gamma-irradiated mutants of scion materials; and genetic transformation employing disease resistance genes. The main results obtained so far include: 12 scion populations consisting in a total of approximately 1,000 individuals; 220 rootstock seedlings (Pyrus communis) under mass selection in Pelotas, RS; 200 P. communis seedlings for rootstocks under field selection in Lages, SC; selection of six promising P. communis rootstocks; establishment of a cultivar evaluation network consisting of five cultivars (?Abate Fetel?, ?Packham?s Triumph?, ?Rocha?, ?Santa Maria? and ?William?s?) shared by all evaluation sites, and other five locality-specific cultivars; and 475 putative mutants under field evaluation. |
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Palavras-Chave: |
Brasil; Cultivar; Melhoramento genético; Programa de Melhoramento Genético; Selection. |
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Thesagro: |
Engenharia Genética; Fruticultura; Germoplasma; Mutagênico; Pera; Seleção; Variedade. |
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Thesaurus NAL: |
genetic engineering; germplasm; mutagenesis; Pyrus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02659naa a2200433 a 4500
001 1910038
005 2012-05-28
008 2011 bl uuuu u00u1 u #d
020 $a978-90-66055-04-9
100 1 $aOLIVEIRA, P. R. D. de
245 $aThe Brazilian pear breeding program.$h[electronic resource]
260 $c2011
500 $aTrabalho apresentado no 11º International Pear Symposium.
520 $aPear production in Brazil reached 17,391 tons in 2008, enough to provide for a mere 20% of the local market. The lack of adapted cultivars is a major obstacle to self-sustainable pear production in the country. In 2006, a novel nationwide pear breeding program was initiated, encompassing several research institutions and aiming to develop cultivars adapted to local growth conditions in an attempt to reverse the current pear importer status of Brazil. The program is structured in distinct research lines, including: enlargement of the genetic variation available in germplasm collections; formation and evaluation of segregating populations, hybrids and selections of scion and rootstock materials; network cultivar agricultural evaluations; screening of gamma-irradiated mutants of scion materials; and genetic transformation employing disease resistance genes. The main results obtained so far include: 12 scion populations consisting in a total of approximately 1,000 individuals; 220 rootstock seedlings (Pyrus communis) under mass selection in Pelotas, RS; 200 P. communis seedlings for rootstocks under field selection in Lages, SC; selection of six promising P. communis rootstocks; establishment of a cultivar evaluation network consisting of five cultivars (?Abate Fetel?, ?Packham?s Triumph?, ?Rocha?, ?Santa Maria? and ?William?s?) shared by all evaluation sites, and other five locality-specific cultivars; and 475 putative mutants under field evaluation.
650 $agenetic engineering
650 $agermplasm
650 $amutagenesis
650 $aPyrus
650 $aEngenharia Genética
650 $aFruticultura
650 $aGermoplasma
650 $aMutagênico
650 $aPera
650 $aSeleção
650 $aVariedade
653 $aBrasil
653 $aCultivar
653 $aMelhoramento genético
653 $aPrograma de Melhoramento Genético
653 $aSelection
700 1 $aRITSCHEL, P. S.
700 1 $aRUFATO, A. De R.
700 1 $aFIORAVANÇO, J. C.
700 1 $aFAORO, I. D.
700 1 $aLEITE, G. B.
700 1 $aDUTRA, L. F.
700 1 $aMAYER, N. A.
700 1 $aDEGENHARDT-GOLDBACH, J.
773 $tActa Horticulturae, The Hague$gn. 909, p. 145-151, out. 2011.
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Embrapa Uva e Vinho (CNPUV) |
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