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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
PEROTTA, J. H.; DYCK, H. R.; OLLHOFF, R. D.; LISBÔA, J. A. N.; VIEIRA, N.; BARROS FILHO, I. R. |
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Afiliação: |
João H. Perotta, Departamento de Medicina Veterinária/Universidade Federal do Paraná - UFPR; Hugo R. Dyck, Departamento de Medicina Veterinária/Universidade Federal do Paraná - UFPR; Rüdiger D. Ollhoff, Universidade Católica do Paraná - UCPR; Júlio A. N. Lisbôa, Departamento de Clínicas Veterinárias/Universidade Estadual de Londrina - UEL; Nilton Vieira, Médico Veterinário Autônomo; Ivan R. Barros Filho, Departamento de Medicina Veterinária/Universidade Federal do Paraná - UFPR. |
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Título: |
One-step laparoscopic abomasopexy versus abomasopexy via right paralumbar fossa to treat left abomasal displacement in dairy cows. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1068-1076, junho 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Abomasopexia por laparoscopia em um passo em comparação à abomasopexia via laparotomia pelo flanco direito no tratamento do deslocamento de abomaso à esquerda em vacas leiteiras. |
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Conteúdo: |
This study aimed to compare the clinical and metabolic results obtained by use of one-step laparoscopic abomasopexy and right paralumbar fossa abomasopexy for the treatment of left displaced abomasum in dairy cows. Thirty Holstein-Friesian dairy cows were randomly placed in two groups: G1, with 15 animals treated by one-step laparoscopic abomasopexy; and G2, with 15 animals treated by right paralumbar fossa ventral abomasopexy. Concentrations of sodium, potassium, chloride, bicarbonate, base excess (BE), pH, partial pressure of carbon dioxide (pCO2) strong ion difference (SID), anion gap (AG), glucose, ?-hydroxybutyrate (BHBA) and non-esterified fatty acids (NEFA) were measured before (M0) and 24 (M1), 48 (M2) and 72 (M3) hours following surgery. Laparotomy was statistically faster than laparoscopy. Hypochloremia was observed only in G2 at M0. Hypokalemia and hypocalcemia were observed in both groups at M0, increasing after surgery. Metabolic alkalosis in both groups before surgery was characterized by high bicarbonate, pCO2, and BE, which decreased in subsequent time points, as well as blood pH. Glucose was statistically increased and NEFA and BHBA were statistically decreased in G2 compared to G1. In G1, NEFA and BHBA decreased significantly following surgery. Both surgical techniques restored abomasal flow and feed intake in both groups. Based in acid-base status, one-step laparoscopy showed no additional advantage in comparison with abomasopexy via right paralumbar fossa. |
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Palavras-Chave: |
Abomasal displacement; Abomasopexia; Abomasopexy; Acid-base status; Bovine; Laparoscopic abomasopexy; Minimally invasive; Minimamente invasiva. |
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Thesagro: |
Abomaso; Bovino; Cirurgia; Equilíbrio Ácido-Base; Laparoscopia; Laparotomia; Vaca Leiteira. |
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Thesaurus Nal: |
Abomasum; Cattle; Dairy cows; Surgery. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182198/1/One-step-laparoscopic-abomasopexy-versus-abomasopexy.pdf
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Marc: |
LEADER 02905naa a2200421 a 4500
001 2094853
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aPEROTTA, J. H.
245 $aOne-step laparoscopic abomasopexy versus abomasopexy via right paralumbar fossa to treat left abomasal displacement in dairy cows.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Abomasopexia por laparoscopia em um passo em comparação à abomasopexia via laparotomia pelo flanco direito no tratamento do deslocamento de abomaso à esquerda em vacas leiteiras.
520 $aThis study aimed to compare the clinical and metabolic results obtained by use of one-step laparoscopic abomasopexy and right paralumbar fossa abomasopexy for the treatment of left displaced abomasum in dairy cows. Thirty Holstein-Friesian dairy cows were randomly placed in two groups: G1, with 15 animals treated by one-step laparoscopic abomasopexy; and G2, with 15 animals treated by right paralumbar fossa ventral abomasopexy. Concentrations of sodium, potassium, chloride, bicarbonate, base excess (BE), pH, partial pressure of carbon dioxide (pCO2) strong ion difference (SID), anion gap (AG), glucose, ?-hydroxybutyrate (BHBA) and non-esterified fatty acids (NEFA) were measured before (M0) and 24 (M1), 48 (M2) and 72 (M3) hours following surgery. Laparotomy was statistically faster than laparoscopy. Hypochloremia was observed only in G2 at M0. Hypokalemia and hypocalcemia were observed in both groups at M0, increasing after surgery. Metabolic alkalosis in both groups before surgery was characterized by high bicarbonate, pCO2, and BE, which decreased in subsequent time points, as well as blood pH. Glucose was statistically increased and NEFA and BHBA were statistically decreased in G2 compared to G1. In G1, NEFA and BHBA decreased significantly following surgery. Both surgical techniques restored abomasal flow and feed intake in both groups. Based in acid-base status, one-step laparoscopy showed no additional advantage in comparison with abomasopexy via right paralumbar fossa.
650 $aAbomasum
650 $aCattle
650 $aDairy cows
650 $aSurgery
650 $aAbomaso
650 $aBovino
650 $aCirurgia
650 $aEquilíbrio Ácido-Base
650 $aLaparoscopia
650 $aLaparotomia
650 $aVaca Leiteira
653 $aAbomasal displacement
653 $aAbomasopexia
653 $aAbomasopexy
653 $aAcid-base status
653 $aBovine
653 $aLaparoscopic abomasopexy
653 $aMinimally invasive
653 $aMinimamente invasiva
700 1 $aDYCK, H. R.
700 1 $aOLLHOFF, R. D.
700 1 $aLISBÔA, J. A. N.
700 1 $aVIEIRA, N.
700 1 $aBARROS FILHO, I. R.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1068-1076, junho 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
06/12/2021 |
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Data da última atualização: |
06/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GIGLIOTI, R.; OKINO, C. H.; AZEVEDO, B. T.; GUTMANIS, G.; KATIKI, L. M.; OLIVEIRA, M. C. de S.; VERCESI FILHO, A. E. |
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Afiliação: |
RODRIGO GIGLIOTI, IZ; CINTIA HIROMI OKINO, CPPSE; BIANCA TAINA AZEVEDO, IZ; GUNTA GUTMANIS, IZ; LUCIANA MORITA KATIKI, IZ; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; ANIBAL EUGENIO VERCESI FILHO, IZ. |
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Título: |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Food Chemistry: Molecular Sciences, v.3, 2021, 100055. |
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Páginas: |
6 p. |
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DOI: |
https://doi.org/10.1016/j.fochms.2021.100055 |
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Idioma: |
Inglês |
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Conteúdo: |
The rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products. |
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Palavras-Chave: |
B Casein; LNA probe; Real time PCR; Sensitivity. |
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Thesaurus NAL: |
Alleles. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228550/1/NovelLNAProbe.pdf
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Marc: |
LEADER 01730naa a2200277 a 4500
001 2137153
005 2021-12-06
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.fochms.2021.100055$2DOI
100 1 $aGIGLIOTI, R.
245 $aNovel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples.$h[electronic resource]
260 $c2021
300 $a6 p.
520 $aThe rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products.
650 $aAlleles
653 $aB Casein
653 $aLNA probe
653 $aReal time PCR
653 $aSensitivity
700 1 $aOKINO, C. H.
700 1 $aAZEVEDO, B. T.
700 1 $aGUTMANIS, G.
700 1 $aKATIKI, L. M.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aVERCESI FILHO, A. E.
773 $tFood Chemistry: Molecular Sciences$gv.3, 2021, 100055.
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Embrapa Pecuária Sudeste (CPPSE) |
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