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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
22/09/1992 |
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Data da última atualização: |
22/09/1992 |
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Autoria: |
KOHL, D. H.; LIN, J. J.; SHEARER, G.; SCHUBERT, K. R. |
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Afiliação: |
Biology Department, Washington University, St. Louis, Missouri 63130. |
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Título: |
Activities of the pentose phosphate pathway and enzymes of proline metabolism in legume root nodules. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Plant Physiology, v.94, p.1258-1264, 1990. |
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Idioma: |
Inglês |
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Conteúdo: |
Based on localization and high activities of pyrroline-5-carboxyiate reductase and proline dehydrogenase activities in soybean nodules, we previously suggested two major roles for pyrroline-5-carboxylate reductase in addition to the production of the considerable quantity of proline needed for biosynthesis: namely, transfer of energy to the location of biological N2 fixation, and production of NADP+ to drive the pentose phosphate pathway. The latter produces ribose-5-phosphate which can be used in de novo purine synthesis required for synthesis of ureides, the majos form in which biologically fixed N2 is transported from soybean root nodules to the plant shoot. In this paper, we report rapid induction (in soybean nodules) and exceptionally high activities (in nodules of eight species of N2-fixing plants) of pentose phosphate pathway and pyrroline-5-carboxylate reuctase. There was a marded increase in proline dehydrogenase activity during soybean (Glycine max) ontogeny. The magnitude of proline dehydrogenase activity in bacteroids of soybean nodles was sufficiently high during most of the time course to supply a significant fraction of the energy requirement for N2 fixation. Proline dehydrogenase activity in bacteroids from nodules of other species was also high. These observations support the above hypothesis. How-ever, comparison of pentose phosphate patway and pyrroline-5-carboxylate reductase activities of ureide versus amide-exporting nodules offers no support. The hypothesis predicts that pyrroline-5-..... MenosBased on localization and high activities of pyrroline-5-carboxyiate reductase and proline dehydrogenase activities in soybean nodules, we previously suggested two major roles for pyrroline-5-carboxylate reductase in addition to the production of the considerable quantity of proline needed for biosynthesis: namely, transfer of energy to the location of biological N2 fixation, and production of NADP+ to drive the pentose phosphate pathway. The latter produces ribose-5-phosphate which can be used in de novo purine synthesis required for synthesis of ureides, the majos form in which biologically fixed N2 is transported from soybean root nodules to the plant shoot. In this paper, we report rapid induction (in soybean nodules) and exceptionally high activities (in nodules of eight species of N2-fixing plants) of pentose phosphate pathway and pyrroline-5-carboxylate reuctase. There was a marded increase in proline dehydrogenase activity during soybean (Glycine max) ontogeny. The magnitude of proline dehydrogenase activity in bacteroids of soybean nodles was sufficiently high during most of the time course to supply a significant fraction of the energy requirement for N2 fixation. Proline dehydrogenase activity in bacteroids from nodules of other species was also high. These observations support the above hypothesis. How-ever, comparison of pentose phosphate patway and pyrroline-5-carboxylate reductase activities of ureide versus amide-exporting nodules offers no support. The hypot... Mostrar Tudo |
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Palavras-Chave: |
C; Fixacao; Fixation; N; N2; Soybean. |
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Thesagro: |
Bradyrhizobium Japonicum; Glycine Max; Metabolismo; Prolina; Soja. |
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Thesaurus Nal: |
metabolism; proline. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02309naa a2200313 a 4500
001 1457856
005 1992-09-22
008 1990 bl --- 0-- u #d
100 1 $aKOHL, D. H.
245 $aActivities of the pentose phosphate pathway and enzymes of proline metabolism in legume root nodules.
260 $c1990
520 $aBased on localization and high activities of pyrroline-5-carboxyiate reductase and proline dehydrogenase activities in soybean nodules, we previously suggested two major roles for pyrroline-5-carboxylate reductase in addition to the production of the considerable quantity of proline needed for biosynthesis: namely, transfer of energy to the location of biological N2 fixation, and production of NADP+ to drive the pentose phosphate pathway. The latter produces ribose-5-phosphate which can be used in de novo purine synthesis required for synthesis of ureides, the majos form in which biologically fixed N2 is transported from soybean root nodules to the plant shoot. In this paper, we report rapid induction (in soybean nodules) and exceptionally high activities (in nodules of eight species of N2-fixing plants) of pentose phosphate pathway and pyrroline-5-carboxylate reuctase. There was a marded increase in proline dehydrogenase activity during soybean (Glycine max) ontogeny. The magnitude of proline dehydrogenase activity in bacteroids of soybean nodles was sufficiently high during most of the time course to supply a significant fraction of the energy requirement for N2 fixation. Proline dehydrogenase activity in bacteroids from nodules of other species was also high. These observations support the above hypothesis. How-ever, comparison of pentose phosphate patway and pyrroline-5-carboxylate reductase activities of ureide versus amide-exporting nodules offers no support. The hypothesis predicts that pyrroline-5-.....
650 $ametabolism
650 $aproline
650 $aBradyrhizobium Japonicum
650 $aGlycine Max
650 $aMetabolismo
650 $aProlina
650 $aSoja
653 $aC
653 $aFixacao
653 $aFixation
653 $aN
653 $aN2
653 $aSoybean
700 1 $aLIN, J. J.
700 1 $aSHEARER, G.
700 1 $aSCHUBERT, K. R.
773 $tPlant Physiology$gv.94, p.1258-1264, 1990.
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Registro original: |
Embrapa Soja (CNPSO) |
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