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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
04/11/2019 |
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Data da última atualização: |
04/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, A. R. de; YAMIN, M.; GAVA, D.; ZANELLA, J. R. C.; GATTI, M. S. V.; BONAFE, C. S. F.; LIMA NETO, D. F. de. |
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Afiliação: |
ANCELMO RABELO DE SOUZA, UNICAMP; MARRIAM YAMIN, UNICAMP; DANIELLE GAVA, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA; MARIA SILVIA VICCARI GATTI, UNICAMP; CARLOS FRANCISCO SAMPAIO BONAFE, UNICAMP; DANIEL FERREIRA DE LIMA NETO, UNICAMP. |
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Título: |
Porcine parvovirus VP1/VP2 on a time series epitope mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Virology Journal, v. 16, art. 75, 2019. |
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DOI: |
10.1186/s12985-019-1165-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for eveloping new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized. |
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Palavras-Chave: |
Epitope prediction; Porcine parvovirus; Spot synthesis. |
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Thesagro: |
Parvovirose; Suinocultura; Virologia. |
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Thesaurus Nal: |
Epitope mapping; Ungulate protoparvovirus 1. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204209/1/final9080.pdf
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Marc: |
LEADER 02185naa a2200301 a 4500
001 2113864
005 2019-11-04
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1186/s12985-019-1165-1$2DOI
100 1 $aSOUZA, A. R. de
245 $aPorcine parvovirus VP1/VP2 on a time series epitope mapping$bexploring the effects of high hydrostatic pressure on the immune recognition of antigens.$h[electronic resource]
260 $c2019
520 $aAbstract: Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for eveloping new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized.
650 $aEpitope mapping
650 $aUngulate protoparvovirus 1
650 $aParvovirose
650 $aSuinocultura
650 $aVirologia
653 $aEpitope prediction
653 $aPorcine parvovirus
653 $aSpot synthesis
700 1 $aYAMIN, M.
700 1 $aGAVA, D.
700 1 $aZANELLA, J. R. C.
700 1 $aGATTI, M. S. V.
700 1 $aBONAFE, C. S. F.
700 1 $aLIMA NETO, D. F. de
773 $tVirology Journal$gv. 16, art. 75, 2019.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/02/2010 |
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Data da última atualização: |
26/08/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SIDER, L. H.; VIEIRA, L. da S.; REGITANO, L. C. de A.; ZAROS, L. G.; NEVES, M. R. M. das; BENVENUTI, C. L.; NAVARRO, A. M. do C.; VILLELA, L. C. V.; CAVALCANTE, A. C. R.; LOBO, R. N. B.; FACO, O.; NICIURA, S. C. M.; PAIVA, S. R.; CAETANO, A. R.; BENAVIDES, M. V.; COUTINHO, L. L.; GARCIA, L. F.; CUNHA, R. M. S da; PINHEIRO, R. R. |
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Afiliação: |
LUCIA HELENA SIDER, CNPC; LUIZ DA SILVA VIEIRA, CNPC; LUCIANA CORREIA DE ALMEIDA REG, CPPSE; Maria Rosalba Moreira das Neves, Universidade Estadual Vale do Acaraú (UVA), Sobral, CE.; Camila Loures Benvenuti, UVA.; Andrine Maria do Carmo Navarro, UVA; LUCIANA CRISTINE VASQUES VILLELA, CNPC; ANTONIO CEZAR ROCHA CAVALCANTE, CNPC; RAIMUNDO NONATO BRAGA LOBO, CNPC; OLIVARDO FACO, CNPC; SIMONE CRISTINA MEO NICIURA, CPPSE; SAMUEL REZENDE PAIVA, CENARGEN; ALEXANDRE RODRIGUES CAETANO, CENARGEN; MAGDA VIEIRA BENAVIDES, CPPSUL; Luiz Lehmann Coutinho, ESALQ-USP; Luiz Fernando Garcia, UNESP-Araçatuba; Rodrigo Maranguape Silva da Cunha, UPESP-Araçatuba; RAYMUNDO RIZALDO PINHEIRO, CNPC. |
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Título: |
Identificação de genes candidatos associados a resistência genética à verminose gastrintestinal em caprinos por meio de ferramentas moleculares e genômicas. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. [Resumos...]. Campo Grande, MS: Embrapa Gado de Corte, 2009. 1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Genômica; Seleção assistida por marcadores. |
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Thesagro: |
Caprino; Genética Molecular; Parasitologia; Verminose. |
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Thesaurus NAL: |
Goats; Molecular genetics; Parasitology. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53392/1/RAC-Identificacao-de-genes.pdf
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Marc: |
LEADER 01389nam a2200421 a 4500
001 1658427
005 2021-08-26
008 2009 bl uuuu u00u1 u #d
100 1 $aSIDER, L. H.
245 $aIdentificação de genes candidatos associados a resistência genética à verminose gastrintestinal em caprinos por meio de ferramentas moleculares e genômicas.$h[electronic resource]
260 $aIn: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. [Resumos...]. Campo Grande, MS: Embrapa Gado de Corte, 2009. 1 CD-ROM.$c2009
650 $aGoats
650 $aMolecular genetics
650 $aParasitology
650 $aCaprino
650 $aGenética Molecular
650 $aParasitologia
650 $aVerminose
653 $aGenômica
653 $aSeleção assistida por marcadores
700 1 $aVIEIRA, L. da S.
700 1 $aREGITANO, L. C. de A.
700 1 $aZAROS, L. G.
700 1 $aNEVES, M. R. M. das
700 1 $aBENVENUTI, C. L.
700 1 $aNAVARRO, A. M. do C.
700 1 $aVILLELA, L. C. V.
700 1 $aCAVALCANTE, A. C. R.
700 1 $aLOBO, R. N. B.
700 1 $aFACO, O.
700 1 $aNICIURA, S. C. M.
700 1 $aPAIVA, S. R.
700 1 $aCAETANO, A. R.
700 1 $aBENAVIDES, M. V.
700 1 $aCOUTINHO, L. L.
700 1 $aGARCIA, L. F.
700 1 $aCUNHA, R. M. S da
700 1 $aPINHEIRO, R. R.
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Embrapa Caprinos e Ovinos (CNPC) |
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