Data/hora: 15/06/2024 15:51:14

Biblioteca(s): | Embrapa Caprinos e Ovinos; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 30/11/2021
Data da última atualização: | 01/12/2021
Autoria: | FONSECA, J. F. da; VERGANI, G. B.; LIMA, M. S. D.; SILVA, K. de M.; MONTEIRO, A. W. U.; RAMOS, A. F.; ALVES, B. R. C.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; BATISTA, R. I. T. P.
Afiliação: | JEFERSON FERREIRA DA FONSECA, CNPC; GABRIEL B. VERGANI, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brazil; MONALISA S. D. LIMA, Universidade Estadual do Ceará (UFC) - Fortaleza, CE, Brazil; KLEIBE DE MORAES SILVA, CNPC; ALEXANDRE WEICK UCHOA MONTEIRO, CNPC; ALEXANDRE FLORIANI RAMOS, Cenargen; BRUNA R. C. ALVES; JOANNA M. G. SOUZA-FABJAN; MARIA E. F. OLIVEIRA; RIBRIO I. T. P. BATISTA, Universidade Federal Fluminense (UFF) - Niterói, Brazil.
Título: | Nonsurgical embryo recovery as a feasible tool for supporting embryo biobanks of Locally Adapted Brazilian sheep and goats.
Ano de publicação: | 2021
Fonte/Imprenta: | Biopreserv Biobank, Nov. 2021.
Descrição Física: | Online ahead of print.
DOI: | doi: 10.1089/bio.2021.0066.
Idioma: | Inglês
Conteúdo: | Abstract: This study assessed the outcomes of nonsurgical embryo recovery (NSER) after superovulation (SOV) in five locally adapted Brazilian breeds of sheep and goats. The objective was to evaluate the feasibility and efficiency of using SOV combined with a less-invasive embryo collection technique for supplying the Brazilian animal gene bank with germplasm from specific genotypes of interest. Morada Nova (n = 20), Santa Inês (n = 20), and Somalis (n = 20) ewes received an intravaginal progesterone (330 mg) device for 9 days, while Canindé (n = 15) and Moxotó (n = 15) goats received an intravaginal medroxyprogesterone acetate (60 mg) device for 6 days. All females received 133 mg of porcine follicle-stimulating hormone (pFSH) administrated in six decreasing doses 12 hours apart, starting 60 hours before device removal, plus 37.5 ?g of d-cloprostenol at the fifth and sixth pFSH dose. Donors in estrus were mated with fertile males. The corpora lutea (CL) number was assessed by ultrasonography 1 day before NSER. On day 6.5 or 7 after estrus, NSER was performed following hormonally induced cervical relaxation. A total of 97% of sheep and 90% of goats responded with estrus, and among those, 91% of sheep and 85% of goats presented a CL. In ewes, the numbers of CL were greater (p < 0.05) in the Santa Inês breed, while similar (p > 0.05) CL numbers were found among the goat breeds. All viable embryos were freezable (excellent and good quality) and the number per donor was 7.8 for sheep and 4.9 for goats. All parameters of NSER efficiency, embryo yield, and fertility post-NSER did not differ (p > 0.05) between breeds among each species. The SOV-NSER procedures applied for an embryo biobank supply of locally adapted Brazilian breeds of small ruminants were efficient regarding production of cryopreservable embryos, and preservation of donor fertility. Therefore, SOV followed by NSER is recommended for embryo biobank assembly in sheep and goats.
Palavras-Chave: | Brazilian Somalis; Canindé; Genetic resources conservation; Morada Nova; Moxotó; NSER; Santa Inês; Transcervical.
Thesaurus Nal: | Animal genetic resources; Animal reproduction; Breeding and Genetic Improvement; Cryopreservation; Embryo transfer; Small ruminants; Superovulation.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)

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CNPC - (39910-1) - UPC - AP - DD -  -  -  - DIS
CENARGEN - (38680-1) - UPC - AP - DD -  -  -  - DIS