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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
27/10/2015 |
Data da última atualização: |
30/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; LIMA, M. R. de; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, CPATU; CLARA SLADE OLIVEIRA, CNPGL; Cláudia Lima Verde Leal, USP; Marina Ragagnin de Lima, UNESP; Maite Del Collado, UNESP / USP; Roberta Vantini, UNESP; Fabio Morato Monteiro, Centro APTA Bovinos de Corte, Instituto de Zootecnia; SIMONE CRISTINA MEO NICIURA, CPPSE; Joaquim Mansano Garcia, UNESP. |
Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, Dec. 2015. |
DOI: |
http://dx.doi.org/10.1017/S0967199414000537 |
Idioma: |
Inglês |
Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
Thesaurus Nal: |
chromatin. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02641naa a2200289 a 4500 001 2027313 005 2022-05-30 008 2015 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1017/S0967199414000537$2DOI 100 1 $aSARAIVA, N. Z. 245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource] 260 $c2015 520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. 650 $achromatin 653 $aBovine 653 $aChemically induced enucleation 653 $aMicrotubule 653 $aNuclear transfer 700 1 $aOLIVEIRA, C. S. 700 1 $aLEAL, C. L. V. 700 1 $aLIMA, M. R. de 700 1 $aCALLADO, M. del 700 1 $aVANTINI, R. 700 1 $aMONTEIRO, F. M. 700 1 $aNICIURA, S. C. M. 700 1 $aGARCIA, J. M. 773 $tZygote$gv. 23, n. 6, p. 852-862, Dec. 2015.
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Embrapa Amazônia Oriental (CPATU) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
11/01/2005 |
Data da última atualização: |
11/01/2005 |
Autoria: |
BARRETO, R. R.; OLIVIERA, M. D.; CALHEIROS, D. F.; SILVA, F. A. V.; MÁRMORA, M. M.; FRANÇOZO, L.; BARBOSA, D.; BARROS, L. F. |
Afiliação: |
UFMS-CPAN (Corumbá, MS); FUNDECT/CNPq; Embrapa Pantanal (Corumbá, MS) PELD/CNPq. |
Título: |
Influência da inundação no Rio Paraguai, em Porto Esperança, sobre as variáveis limnológicas. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: SIMPÓSIO SOBRE RECURSOS NATURAIS E SÓCIO-ECONÔMICOS DO PANTANAL, 4., 2004, Corumbá, MS. Sustentabilidade regional: anais. Corumbá: Embrapa Pantanal: UCDB: UFMS: SEBRAE-MS, 2004. (CD-ROM). |
Idioma: |
Português |
Conteúdo: |
O Pantanal, com área aproximada de 140.000 km², faz parte da bacia hidrográfica do rio
Paraguai e tem sua maior parte em território brasileiro. O principal rio do Pantanal, o rio
Paraguai, possui uma extensão de 2.621 Km, tem como principais tributários os rios Jauru. Cabaçal, Sepotuba, Cuiabá, São Lourenço, Taquari, Miranda e Aquidauana. O contato do rio Paraguai com a extensa planície de inundação causa mudanças expressivas nas características da água durante as fases do ciclo hidrológico. Este trabalho tem como objetivo verificar a influência da inundação sobre as variáveis limnológicas em dois anos com níveis de cheias diferentes. |
Palavras-Chave: |
Dequada; Rio Paraguai. |
Thesagro: |
Limnologia. |
Thesaurus NAL: |
limnology; Pantanal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01533naa a2200265 a 4500 001 1811491 005 2005-01-11 008 2004 bl uuuu u00u1 u #d 100 1 $aBARRETO, R. R. 245 $aInfluência da inundação no Rio Paraguai, em Porto Esperança, sobre as variáveis limnológicas. 260 $c2004 520 $aO Pantanal, com área aproximada de 140.000 km², faz parte da bacia hidrográfica do rio Paraguai e tem sua maior parte em território brasileiro. O principal rio do Pantanal, o rio Paraguai, possui uma extensão de 2.621 Km, tem como principais tributários os rios Jauru. Cabaçal, Sepotuba, Cuiabá, São Lourenço, Taquari, Miranda e Aquidauana. O contato do rio Paraguai com a extensa planície de inundação causa mudanças expressivas nas características da água durante as fases do ciclo hidrológico. Este trabalho tem como objetivo verificar a influência da inundação sobre as variáveis limnológicas em dois anos com níveis de cheias diferentes. 650 $alimnology 650 $aPantanal 650 $aLimnologia 653 $aDequada 653 $aRio Paraguai 700 1 $aOLIVIERA, M. D. 700 1 $aCALHEIROS, D. F. 700 1 $aSILVA, F. A. V. 700 1 $aMÁRMORA, M. M. 700 1 $aFRANÇOZO, L. 700 1 $aBARBOSA, D. 700 1 $aBARROS, L. F. 773 $tIn: SIMPÓSIO SOBRE RECURSOS NATURAIS E SÓCIO-ECONÔMICOS DO PANTANAL, 4., 2004, Corumbá, MS. Sustentabilidade regional: anais. Corumbá: Embrapa Pantanal: UCDB: UFMS: SEBRAE-MS, 2004. (CD-ROM).
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