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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
07/03/2012 |
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Data da última atualização: |
21/10/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; UNESP/JABOTICABAL. |
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Título: |
Effects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO, 2012 |
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Páginas: |
p. 119 |
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Idioma: |
Português |
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Conteúdo: |
Enucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable. MenosEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated... Mostrar Tudo |
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Palavras-Chave: |
Demecolcine; Meiotic cell cycle. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03762nam a2200217 a 4500
001 1917825
005 2015-10-21
008 2012 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aEffects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation.
260 $aIn: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO$c2012
300 $ap. 119
520 $aEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable.
650 $aoocytes
653 $aDemecolcine
653 $aMeiotic cell cycle
700 1 $aOLIVEIRA, C. S.
700 1 $aTETZNER, T. A. D.
700 1 $aLIMA, M. R. de
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 35 | |
| 9. |  | LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. The use of linoleic acid in in vitro culture of bovine embryos and its effects on production and survival to vitrification. Animal Reproduction, v. 14, n. 3, p. 894, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | GARCIA, J. M.; LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S. Evaluation of L-carnitine supplementation on the production and vitrification of bovine embryos produced in vitro. Animal Reproduction, v. 14, n. 3, p. 882, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | SANTOS, H. P. dos; LHAMBY, J. C. B.; PRESTES, A. M.; LIMA, M. R. de. Efeito de manejos de solo e de rotação de culturas de inverno no rendimento e doenças de trigo. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 12, p. 2355-2361, dez. 2000.| Biblioteca(s): Embrapa Trigo; Embrapa Unidades Centrais. |
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| 14. | | BEZERRA, K. S.; SANTOS, A. J. G.; LEITE, M. R.; SILVA, A. M. da; LIMA, M. R. de. Crescimento e sobrevivência da tilápia chitralada submetida a diferentes fotoperíodos. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 6, p. 737-743, jun. 2008 Título em inglês: Growth and survival of tilapia chitralada submitted to different photoperiods.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 16. | | SARAIVA, N. Z.; FIGUEIRO, M. R.; MARQUES, J. R. F.; LIMA, M. R. de; GARCIA, J. M. Effect of in vitro co-culture of buffalo embryos with bovine cumulus cells on the potential for early embryonic development. Animal Reproduction, v. 14, n. 3, p. 858, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; LIMA, M. R. DE; GARCIA, J. M. Global H3K9AC and H3K27ME3 expression in blastomeres from 8-to 16-cell stage bovine embryos. Reproduction, Fertility and Development , v. 26, n. 1, p. 148-149, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; LIMA, M. R. de; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; GARCIA, J. M.; BORGES, C. A. V.; CAMARGO, L. S. de A. Cell death is involved in sexual dimorphism during preimplantation development. Mechanisms of Development v. 139, p. 42-50, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 19. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; MELO, D. S. de; NICIURA, S. C. M.; GARCIA, J. M. Chemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer. Cellular Reprogramming, v. 14, n. 5, p. 1-11, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | RODRIGUES, V. G. S.; COSTA, R. S. C. da; LEÔNIDAS, F. das C.; SANTOS, M. R. A. dos; LIMA, M. R. de. Efeito da arborização na cobertura do solo em agrossistemas com café robusta (Coffea canephora) em Machadinho Do Oeste-RO. In: REUNIÃO AMAZÔNICA DE AGROECOLOGIA, 1., 2007, Manaus. A agroecologia no contexto amazônico: palestras, relatos de experiência e resumos. Manaus: Embrapa Amazônia Ocidental, 2007. 1 CD ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Rondônia. |
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| Registros recuperados : 35 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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