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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/04/2005 |
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Data da última atualização: |
27/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A. |
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Afiliação: |
CNPSO. |
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Título: |
Is the Nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells? |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington, 2003. |
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Páginas: |
p. 81-82. |
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Idioma: |
Inglês |
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Conteúdo: |
Anticarsia gemmatalis, is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increasing use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that the midgut is considered one of the most important barriers against viral invasion, before systemic infections can be caused in various tissues if the virus succeeds in reaching the host larva hemocele. Our work aimed to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 occlusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cell were the most affected ones among the different midgut epithelial cells. In the SL, these cells showed many morphological signs of cellular damage from 24 hours after infection, mainly at the proximal midgut region. However, in RL these cells exhibited minor morphological damage up to 120 hours. Polyhedra were visualized in the midgut of both SL an RL from 96 hours of infection, but the amount of viral structures were always higher in SL, affecting the midgut epithelial and tracheal cells, as well as attached hemocytes (mainly plasmatocytes and granulocytes). Our results showed that the AgMNPV, in fact, invaded RL cells. However, the virus did not affect these insects as they did with the susceptible ones, allowing development and survival of resistant larvae. The mechanism interfering with the virus infection progress in midgut cells of RL it still unknown. This work has been supported by FAPESP and PRONEX (MCT/Finep/CNPq). MenosAnticarsia gemmatalis, is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increasing use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that the midgut is considered one of the most important barriers against viral invasion, before systemic infections can be caused in various tissues if the virus succeeds in reaching the host larva hemocele. Our work aimed to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 occlusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cell were the most affected ones among the different midgut epithelial cells. In the SL, these cells showed many morphological s... Mostrar Tudo |
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Thesagro: |
Baculovirus; Controle Biológico; Praga de Planta; Resistência; Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03077nam a2200229 a 4500
001 1467873
005 2020-02-27
008 2003 bl uuuu u00u1 u #d
100 1 $aLEVY, S. M.
245 $aIs the Nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells?$h[electronic resource]
260 $aIn: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 36., 2003, Burlington. Program and abstracts... Burlington$c2003
300 $ap. 81-82.
520 $aAnticarsia gemmatalis, is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increasing use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that the midgut is considered one of the most important barriers against viral invasion, before systemic infections can be caused in various tissues if the virus succeeds in reaching the host larva hemocele. Our work aimed to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 occlusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cell were the most affected ones among the different midgut epithelial cells. In the SL, these cells showed many morphological signs of cellular damage from 24 hours after infection, mainly at the proximal midgut region. However, in RL these cells exhibited minor morphological damage up to 120 hours. Polyhedra were visualized in the midgut of both SL an RL from 96 hours of infection, but the amount of viral structures were always higher in SL, affecting the midgut epithelial and tracheal cells, as well as attached hemocytes (mainly plasmatocytes and granulocytes). Our results showed that the AgMNPV, in fact, invaded RL cells. However, the virus did not affect these insects as they did with the susceptible ones, allowing development and survival of resistant larvae. The mechanism interfering with the virus infection progress in midgut cells of RL it still unknown. This work has been supported by FAPESP and PRONEX (MCT/Finep/CNPq).
650 $aSoybeans
650 $aBaculovirus
650 $aControle Biológico
650 $aPraga de Planta
650 $aResistência
650 $aSoja
700 1 $aFALLEIROS, A. M. F.
700 1 $aMOSCARDI, F.
700 1 $aGREGÓRIO, E. A.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 37 | |
| 4. |  | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A. Differences in the peritrophic membrane of susceptible and resistant Anticarsia gemmatalis larvae to the insect nucleopolyhedrovirus (AgMNPV). In: INTERNATIONAL COLLOQUIUM ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL, 8.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS 6.; ANNUAL MEETING OF THE SIP, 35., 2002, Foz do Iguassu. Program and abstracts. Londrina: Embrapa Soja: Embrapa Recursos Genéticos e Biotecnologia: UEL: SIP, 2002. p. 43-44. (Embrapa Soja. Documentos, 183; Embrapa Recursos Genéticos e Biotecnologia, 73).| Biblioteca(s): Embrapa Soja. |
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| 6. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A. Is the nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells? In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 293. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 7. | | LEVY, S. M.; FALLEIROS, A. M. F.; BASSO, N. A.; MOSCARDI, F.; GREGÓRIO, E. A. Morfologia do intestino médio de Anticarsia gemmatalis, susceptível e resistente ao vírus da poliedrose nuclear (AgMNPV), em larvas controle e infectadas pelo virus. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 9., 2005, Recife. Anais... Recife, 2005. p. 188.| Biblioteca(s): Embrapa Soja. |
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| 8. | | LEVY, S. M.; MOSCARDI, F.; FALLEIROS, A. M. F.; SILVA, R. J.; GREGÓRIO, E. A. Morfometria do epitélio do intestino médio de Anticarsia gemmatalis (Hübner,1818): estudo comparativo entre larvas suscetíveis e resistentes ao AgMNPV. In: SICONBIOL, 10., 2007, Brasília, DF. Inovar para preservar a vida: [palestras e resumos]. Brasília, DF: Sociedade Entomológica do Brasil, 2007. 1 CD-ROM. Resumo pdf. 102.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A.; TOLEDO, L. A. Morphological study of the hindgut in larvae of Anticarsia gemmatalis Hubner (Lepidoptera: Noctuidae). Neotropical Entomology, Londrina, v. 33, n. 4, p. 427-431, July/Aug. 2004.| Biblioteca(s): Embrapa Soja. |
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| 12. | | MATSUBARA, F. H.; MARQUES, C. R. G.; LEVY, S. M.; MOSCARDI, F.; FALLEIROS, A. M. F. Estudo dos hemócitos de larvas de Anticarsia gemmtalis (Hübner, 1818) (Lepidoptera: Noctuidae) suscetíveis e resistentes ao AgMNPV à microscopia eletrônica de varredura. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 1314-2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | CRUZ, N. A.; HOFFMANN-CAMPO, C. B.; LEVY, S. M.; FALLEIROS, A. M. F. Efeitos de variedades de soja no intestino médio de lagartas de Anticarsia gemmatalis (Hübner,1818) (Lepidoptera: Erebidae) resistentes ao vírus AgMNPV. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 9., Maceio, 2016. Anais... Brasília, DF: Embrapa, 2016. p. 128. Editores técnicos: Elio Cesar Guzzo, Marcus Vinicius Sampaio, Jader Braga Maia, Aldomário Santo Negrisoli Junior.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 15. | | MATSUBARA, F. H.; CRUZ, N. A.; LEVY, S. M.; MOSCARDI, F.; FALLEIROS, A. M. F. Histoquímica em hemócitos de larvas de Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Noctuidae) suscetíveis e resistentes ao AgMNPV. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação: anais. Viçosa, MG: UFV, 2008. 1 CD-ROM. Resumo 1314-1.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A.; TOLEDO, L. A. Ultramorphology of digestive tract of Anticarsia gemmatalis (Hübner, 1818) (Lepidoptera: Noctuidae) at final larval development. Semina: Ciências Agrárias, Londrina, v. 29, n. 2, p. 313-322, abr./jun. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; ANDRADE, F. G.; GREGÓRIO, E. A. Ultrastructure of the endocrine cels in the midgut of Anticarsia gemmatalis (Lepidoptera) larvae. Brazilian Journal of Morphological Sciences, São Paulo, v. 22, p. 189, 2005. Supplement. Edição dos Resumos do XX Congress of the Brazilian Society for Microscopy and Microanalysis, Águas de Lindóia, SP, Agosto, 2005.| Biblioteca(s): Embrapa Soja. |
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| 20. | | MOSCARDI, M. L.; MARTINS, M. R.; PINHEIRO, D. O.; LEVY, S. M.; ROGGIA, S.; FALLEIROS, A. M. F. Aspectos biológicos de Euschistus heros alimentado com cultivares de soja Bt e resistentes a percevejos. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 16., 2019, Londrina. Controle biológico: da academia ao campo, rumo à sustentabilidade: anais. Londrina: Sociedade Entomológica do Brasil: Embrapa Soja: Universidade Estadual de Londrina, 2019. 495 p. Organizadores: Adeney de Freitas Bueno, Pedro Manuel Oliveira Janeiro, Michele Potrich, Geraldo Andrade Carvalho. p. 430.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 37 | |
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