Data/hora: 14/06/2024 06:37:31

Biblioteca(s): | Embrapa Pecuária Sudeste.
Data corrente: | 09/11/2006
Data da última atualização: | 13/06/2023
Autoria: | NOGUEIRA, A. R. de A.; MOTA, A. V. C.; LEMOS, S. G.; PARRA, A.; ALONSO, J.
Afiliação: | ANA RITA DE ARAUJO NOGUEIRA, CPPSE; ARIEL VICENTE CAMARNADO MOTA, UFSCar/SÃO CARLOS, SP.; SHERLAN G. LEMOS, UFSCar/SÃO CARLOS, SP.; ALEIX PARRA, UNIVIERSITAT DE BARCELONA; JULIAN ALONSO, UNIVIERSITAT DE BARCELONA.
Título: | Flow injection potentiometric system for ATP determination in pharmaceutical samples using experimental design.
Ano de publicação: | 2006
Fonte/Imprenta: | In: INTERNATIONAL CONFERENCE FLOW ANALYSIS, 10., 2006, Porto. Proceedings... Porto: IAFA, 2006.
Páginas: | p. 183.
Idioma: | Inglês
Conteúdo: | To identify thenítrogen transportation formsand attaín lhe control mechanísm, lhe amount of free amino acids in different parts of lhe plant has to be determined. An automatic, fast and relíable procedure multícomutated flow system [1] has been developed for spectrophotometríc analysís of totalamíno acíds in plant material. The method is suitable for routine analysís for a largenumber of samples of plant material. The flow manifold was designed with computer- controlled three-way solenoíd valves forindependent handlíng of sample and reagent solutions and a data acquisition system from a spectrophotometer, employed for sígnal measurements. The software for system control was performed bya program with use of a LabView platform (Nationallntrumentsl [2]. The detection reaction was based on the complexation of amino functíonal groups ofamino acids by nínhydrin. It reacts with free a-amino groups, producing the colored ninhydrin chromophore called Ruhemann's purple (RP) IAmax = 570 nm; (= 22000) [3]. The proposed detection system shows a linear range concentration upto 2.0 x 10-3 mal L-1 wíth coefficient of variation of 1.1% (n = 10). Detection limíts wereestimated as 2.8 x 10-3 mal L-1 at 99.7% confidence level for total amino acids, and a mean sampling rate of 30 determínQtions per hour was achieved.
Palavras-Chave: | Flow system; Plant; Spectrometric.
Thesaurus Nal: | amino acids.
Categoria do assunto: | W Química e Física
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (16449-1) - UPC - RA - DD - PROCI-2006.00094 - NOG - 2006.00094 - DIS