Data/hora: 01/06/2024 16:32:44

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 25/11/2008
Data da última atualização: | 02/08/2019
Autoria: | GONZÁLEZ, T. O.; CAMPANHARO, J. C.; LEMOS, E. G. de M.
Afiliação: | Tehuni Orlando González, Universidad Central de Venezuela/Facultad de Agronomía; João Carlos Campanharo, Universidade Estadual Paulista/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia; Eliana Gertrudes de Macedo Lemos, Universidade Estadual Paulista/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia.
Título: | Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean.
Ano de publicação: | 2008
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 9, p. 1177-1184, set. 2008.
Idioma: | Inglês
Notas: | Título em português: Caracterização genética e capacidade de fi xação de nitrogênio de estirpes de Rhizobium em feijoeiro.
Conteúdo: | This study aimed to genetically characterize four new Rhizobium strains, and to evaluate their nodulation and fixation capacity compared to commercial strains and to native rhizobia population of a Brazilian Rhodic Hapludox. Two experiments were carried out in randomized blocks design, under greenhouse conditions, in 2007. In the first experiment, the nodulation and nitrogen fixation capacity of new strains were evaluated, in comparison to the commercial strains CIAT-899 and PRF-81 and to native soil population. It was carried out in plastic tubes filled with vermiculite. DNA extractions and PCR sequencing of the intergenic space were made from the isolated pure colonies, in order to genetically characterize the strains and the native rhizobia population. In the second experiment, the nodulation and productivity of common beans Perola cultivar were determined, with the use of evaluated strains, alone or in mixture with PRF-81 strain. It was carried out in pots filled with soil. The native soil population was identified as Rhizobium sp. and was inefficient in nitrogen fixation. Three different Rhizobium species were found among the four new strains. The LBMP-4BR and LBMP-12BR new strains are among the ones with greatest nodulation and fixation capacity and exhibit differential responses when mixed to PRF-81.
Palavras-Chave: | biological nitrogen fixation; espaço intergênico; fixação biológica de nitrogênio; intergenic space; população nativa de rizóbios; seqüenciamento; sequencing; soil rhizobia population.
Thesagro: | Filogenia; Phaseolus Vulgaris.
Thesaurus Nal: | phylogeny.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/106337/1/Genetic-characterization.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (44941-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS