Data/hora: 18/06/2024 18:01:37

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 13/08/2021
Data da última atualização: | 15/09/2021
Autoria: | SOUZA, V. das G. P. de; SOUZA, G. T. de; LEMOS, D. R. de; GUIMARAES, J. M. de O.; QUINTAO, C. C. R.; MUNK, M.; SARAIVA, N. Z.; CAMARGO, L. S. de A.
Afiliação: | VANESSA DAS GRAÇAS PEREIRA DE SOUZA, Universidade Federal de Juiz de Fora; GUSTAVO TORRES DE SOUZA, Universidade Federal de Juiz de Fora; DIANA RANGEL DE LEMOS, Universidade Federal de Juiz de Fora; JUDITH MARIA DE OLIVEIRA GUIMARÃES, Universidade Federal de Juiz de Fora; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; MICHELE MUNK, Universidade Federal de Juiz de Fora; NAIARA ZOCCAL SARAIVA, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.
Título: | Heat shock during in vitro maturation of bovine oocytes disturbs bta-miR-19b and DROSHA transcripts abundance after in vitro fertilization.
Ano de publicação: | 2021
Fonte/Imprenta: | Reproduction in Domestic Animals, v. 56, n. 8, p. 1128-1136, 2021.
DOI: | https://doi.org/10.1111/rda.13956
Idioma: | Inglês
Conteúdo: | While microRNAs (miRNAs) are a class of non-coding RNAs important for embryo development, the relationship between them and heat stress during oocyte maturation has not yet been established. This study investigated the effect of heat shock during in vitro maturation (IVM) on the abundance of bta-miR-20a, -27b, -103, -21-5p, -19b, -1246 miRNAs and DROSHA and DICER1 mRNAs, previously reported for being involved in oocyte maturation, response to heat stress and miRNA biogenesis. Oocytes were exposed for 12h to heat shock during IVM, fertilized in vitro and the presumptive zygotes cultured for eight days. The relative quantification of miRNAs and mRNAs was performed by real-time PCR in vitro-matured oocytes and 8-cell stage embryos. Progression of meiosis, embryonic development and apoptotic indices was also evaluated. Heat shock compromised (p < .05) oocyte nuclear maturation, cleavage and embryo development, with a higher (p < .05) embryonic apoptotic index than the control group. The abundance of bta-miR-19b increased (p < .05) whereas the abundance of DROSHA transcripts decreased (p < .05) in embryos derived from heat-shocked oocytes. In conclusion, heat shock during IVM influences the abundance of bta-miR-19b and DROSHA in pre-implantation embryos, indicating a persistent effect of heat shock that can be associated with impaired embryo development.
Palavras-Chave: | Epigenética; Estresse por calor.
Thesagro: | Bovino; Embrião Animal; Reprodução Animal.
Thesaurus Nal: | Embryo (animal); Epigenetics; Heat stress; MicroRNA.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (25205-1) - UPC - AP - DD -  -  -  - DIS