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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
26/07/2018 |
Data da última atualização: |
26/07/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LIMA, J. T. M.; FONSECA, J. F. da; BALARO, M. F. A.; ESTEVES, L. V.; ASCOLI, F. O.; LEITE, R. C.; RIBEIRO, A. C. S.; DELGADO, K. F.; SOUZA-FABJAN, J. M. G.; TORRES FILHO, R. A.; BRANDÃO, F. Z. |
Afiliação: |
Peixoto, Renato Mesquita Peixoto, Renato Mesquita2, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; Jeferson Ferreira da Fonseca, CNPC; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil.; UFF - Niterói, RJ, Brazil. |
Título: |
Effect of natural mating or laparoscopic artificial insemination in superovulated Santa Inês ewes on superovulatory response, fertility and embryo viability. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Animal Production Science, v. 56, n. 9, p. 1463-1468, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 300 IU eCG and 0.0375 mg d-cloprostenol were given. Twelve hours after sponge removal, 0.025 mg gonadotropin-releasing hormone was administered. Superovulation started 48 h after gonadotropin-releasing hormone treatment, using 5 IU/kg follicle-stimulating hormone (pFSH). At the first pFSH dose, new sponges were inserted. At the fifth dose, 0.0375 mg cloprostenol was administered and the sponges were removed. The GNM was mated with rams every 12 h, until the end of oestrus. The ewes of GAI were laparoscopic inseminated with frozen?thawed semen 36 and 48 h after sponge removal. Ultrasonography was performed every 24 h from the beginning of oestrus synchronisation treatment and every 12 h from the second sponge removal to 2 days after the last pFSH dose. Six to seven days after mating, the number of corpora lutea (CL) was evaluated by laparoscopy and the females with >4CL were subjected to embryo collection. The interval from sponge removal to ovulation was shorter (P < 0.05) in the GNM. The overall superovulatory response was 63.3% (19/30), with 60.0% and 66.7% in GNM and GAI, respectively (P > 0.05). The number of recovered structures (6.4 ± 2.4 vs 4.5 ± 3.0), recovery rate (74.0 ± 16.0 vs 52.3 ± 26.5%), number of transferable embryos (3.0 ± 2.9 vs 3.6 ± 2.0) and viability rate (47.2 ± 45.3 vs 77.4 ± 37.1%) did not differ between GAI and GNM (P > 0.05). However, the GAI group showed a higher (P < 0.05) number of unfertilised oocytes (3.1 ± 3.1) and a higher non-fertilisation rate (47.1 ± 45.3%) than the GNM (0.9 ± 2.1 and 11.5 ± 21.5%). The mating method did not affect the superovulatory response, and production of viable embryos although the non-fertilisation rate has been inferior for the AI group. MenosAbstract: This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 300 IU eCG and 0.0375 mg d-cloprostenol were given. Twelve hours after sponge removal, 0.025 mg gonadotropin-releasing hormone was administered. Superovulation started 48 h after gonadotropin-releasing hormone treatment, using 5 IU/kg follicle-stimulating hormone (pFSH). At the first pFSH dose, new sponges were inserted. At the fifth dose, 0.0375 mg cloprostenol was administered and the sponges were removed. The GNM was mated with rams every 12 h, until the end of oestrus. The ewes of GAI were laparoscopic inseminated with frozen?thawed semen 36 and 48 h after sponge removal. Ultrasonography was performed every 24 h from the beginning of oestrus synchronisation treatment and every 12 h from the second sponge removal to 2 days after the last pFSH dose. Six to seven days after mating, the number of corpora lutea (CL) was evaluated by laparoscopy and the females with >4CL were subjected to embryo collection. The interval from sponge removal to ovulation was shorter (P < 0.05) in the GNM. The overall superovulatory response was 63.3% (19/30), with 60.0% and 66.7% in GNM and GAI,... Mostrar Tudo |
Palavras-Chave: |
Estrus synchronisation; MOET; Oestrus synchronization; Raça Santa Inês. |
Thesagro: |
Inseminação Artificial; Ovino; Reprodução Animal; Superovulação; Transferência de Embrião. |
Thesaurus Nal: |
Artificial insemination; Embryo transfer; Sheep; Superovulation. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03386naa a2200397 a 4500 001 2093593 005 2018-07-26 008 2016 bl uuuu u00u1 u #d 100 1 $aLIMA, J. T. M. 245 $aEffect of natural mating or laparoscopic artificial insemination in superovulated Santa Inês ewes on superovulatory response, fertility and embryo viability.$h[electronic resource] 260 $c2016 520 $aAbstract: This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 300 IU eCG and 0.0375 mg d-cloprostenol were given. Twelve hours after sponge removal, 0.025 mg gonadotropin-releasing hormone was administered. Superovulation started 48 h after gonadotropin-releasing hormone treatment, using 5 IU/kg follicle-stimulating hormone (pFSH). At the first pFSH dose, new sponges were inserted. At the fifth dose, 0.0375 mg cloprostenol was administered and the sponges were removed. The GNM was mated with rams every 12 h, until the end of oestrus. The ewes of GAI were laparoscopic inseminated with frozen?thawed semen 36 and 48 h after sponge removal. Ultrasonography was performed every 24 h from the beginning of oestrus synchronisation treatment and every 12 h from the second sponge removal to 2 days after the last pFSH dose. Six to seven days after mating, the number of corpora lutea (CL) was evaluated by laparoscopy and the females with >4CL were subjected to embryo collection. The interval from sponge removal to ovulation was shorter (P < 0.05) in the GNM. The overall superovulatory response was 63.3% (19/30), with 60.0% and 66.7% in GNM and GAI, respectively (P > 0.05). The number of recovered structures (6.4 ± 2.4 vs 4.5 ± 3.0), recovery rate (74.0 ± 16.0 vs 52.3 ± 26.5%), number of transferable embryos (3.0 ± 2.9 vs 3.6 ± 2.0) and viability rate (47.2 ± 45.3 vs 77.4 ± 37.1%) did not differ between GAI and GNM (P > 0.05). However, the GAI group showed a higher (P < 0.05) number of unfertilised oocytes (3.1 ± 3.1) and a higher non-fertilisation rate (47.1 ± 45.3%) than the GNM (0.9 ± 2.1 and 11.5 ± 21.5%). The mating method did not affect the superovulatory response, and production of viable embryos although the non-fertilisation rate has been inferior for the AI group. 650 $aArtificial insemination 650 $aEmbryo transfer 650 $aSheep 650 $aSuperovulation 650 $aInseminação Artificial 650 $aOvino 650 $aReprodução Animal 650 $aSuperovulação 650 $aTransferência de Embrião 653 $aEstrus synchronisation 653 $aMOET 653 $aOestrus synchronization 653 $aRaça Santa Inês 700 1 $aFONSECA, J. F. da 700 1 $aBALARO, M. F. A. 700 1 $aESTEVES, L. V. 700 1 $aASCOLI, F. O. 700 1 $aLEITE, R. C. 700 1 $aRIBEIRO, A. C. S. 700 1 $aDELGADO, K. F. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aTORRES FILHO, R. A. 700 1 $aBRANDÃO, F. Z. 773 $tAnimal Production Science$gv. 56, n. 9, p. 1463-1468, 2016.
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Registros recuperados : 6 | |
3. | | TOVAR, G. P. B.; SOUZA, M. L.; ROCHA, T. L.; SIHLER, W.; VALENCIA, A. J.; ARBOLEDA, J. W. V. Biological activity of poriferans extracts from the Colombian Caribbean on Meloidogyne incognita and Spodoptera frugiperda IPLB-SF-21 cell line. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 14., 2015, Teresópolis. [Resumos]. Londrina: Sociedade Entomológica do Brasil, 2015. Sinconbiol.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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4. | | LIMA, J. N.; OLIVEIRA NETO, O. B.; VALENÇA, J. A.; ARBOLEDA, J. W.; MULINARI, F.; GROSSI DE SÁ, M. F. Uso potencial do inibidor de alfa-amilase de trigo 0.53 no controle de bruquídeos. In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 2., 2007, Brasília, DF. II Workshop Interação Molecular Planta-Praga. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 16-20 (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 229).Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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5. | | LIMA, J. N.; MULINARI, F.; OLIVEIRA-NETO, O. B.; VALENÇA, A. J.; ARBOLEDA, J. W.; GROSSI-DE-SÁ, M. F. Uso potencial do inibidor de alpha-amilase de trigo 0.53 no controle de bruquídeos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 95.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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6. | | NORIEGA, D. D.; ARIAS, P. L.; BARBOSA, H. R.; ARRAES, F. B. M.; OSSA, G. A.; VILLEGAS, B.; COELHO, R. R.; ALBUQUERQUE, E. V. S.; TOGAWA, R. C.; GRYNBERG, P.; WANG, H.; VÉLEZ, A. M.; ARBOLEDA, J. W.; GROSSI-DE-SA, M. F.; SILVA, M. C. M.; VALENCIA-JIMÉNEZ, A. Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei. Scientific Reports, v.9, n. 1, article 12804, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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