Data/hora: 01/06/2024 10:01:44

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	17/03/2009	
Data da última atualização: 	08/02/2024	
Autoria: 	JOSÉ, A. A. F. B. V.; GAMA, M. A. S.; LANNA, D. P. D.	
Afiliação: 	A. A. F. B. V. José, ESALQ/USP; Marco Antonio Sundfeld da Gama, Embrapa Gado de Leite; Dante Pazzanese Duarte Lanna, ESALQ/USP.	
Título: 	Effects of trans - 10, cis - 12 conjugated linoleic acid on gene expression and lipid metabolism of adipose tissue of growing pigs.	
Ano de publicação: 	2008	
Fonte/Imprenta: 	Genetics and Molecular Research, v. 7, n. 2, p. 284-294, 2008.	
DOI: 	https://doi.org/10.4238/vol7-2gmr417	
Idioma: 	Inglês	
Conteúdo: 	The objective of the present study was to determine the effects of trans-10, cis-12 conjugated linoleic acid (CLA) in adipose tissue explant cultures of growing pigs on the following responses: lipogenesis (measured as rate of 14C-labeled glucose incorporation over a subsequent 2-h incubation in the presence or absence of insulin), lipolysis (release of non-esterified fatty acid over a 2-h incubation in the presence or absence of isoproterenol), activities of lipogenic enzymes, and mRNA abundance of fatty acid synthase (FAS). Adipose tissue explants from nine growing pigs (78 ± 3 kg) were cultured in 199 medium with insulin, dexamethasone and antibiotics for 4, 12, 24, and 48 h. The treatments were 1) control: 100 µM polyvinyl alcohol (PVA); 2) pGH: 100 ng/mL porcine growth hormone (pGH) plus 100 µM PVA; 3) CLA200: 200 µM trans-10, cis-12 CLA; 4) CLA50: 50 µM trans-10, cis-12 CLA, and 5) LA: 200 µM linoleic acid. Fatty acids were added along with PVA (2:1), respectively, for 24 h. Explants were collected after each culture period and assayed for lipogenesis. Transcripts of FAS mRNA were quantified by real-time RT-PCR after 24 and 48 h. Lipolysis and activities of FAS, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and NADP-malate dehydrogenase were determined after 48 h. As expected, glucose incorporation was decreased (P < 0.05) in response to pGH treatment (positive control). LA had no effect on any parameter evaluated. Treatment with trans-10, cis-12 CLA decreased FAS activity (P < 0.05), but NADPH-generating enzymes were unaffected by treatments. Consistent with reduction in FAS activity, both lipid synthesis and FAS mRNA abundance were reduced with chronic CLA treatment, pGH increased baseline and stimulated lipolysis (P < 0.05) after 48 h of culture, while CLA treatment had no effect on nonesterified fatty acid release. Results of this study showed that trans-10, cis-12 CLA alters lipogenesis but has no effect on lipolysis in cultures of pig adipose tissue.	
Palavras-Chave: 	Adipose tissue explants; Bioactive fatty acid.	
Thesaurus Nal: 	conjugated linoleic acid; lipogenesis; swine.	
Categoria do assunto: 	--	
URL: 	http://www.funpecrp.com.br/gmr/year2008/vol7-2//pdf/gmr417.pdf	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (16229-1) - UPC - AP - DD -  -  -  - DIS