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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/03/2011 |
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Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARCURI, E. F.; ÂNGELO, F. F.; TALON, R.; BORGES, M. de F.; LEROY, S.; LOISEAU, G.; LANGE, C. C.; ANDRADE, N. J. de; MONTET, D. |
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Afiliação: |
EDNA FROEDER ARCURI, CNPGL; FABÍOLA FONSECA ÂNGELO, UFV; RÉGINE TALON, Institut National de la Recherche Agronomique; MARIA DE FATIMA BORGES, CNPAT; SABINE LEROY, Institut National de la Recherche Agronomique; GÉRARD LOISEAU, Centre de Coopération Internationale en Recherche Agronomique pour le Dévelopment; CARLA CHRISTINE LANGE, CNPGL; NÉLIO JOSÉ DE ANDRADE, UFV; DIDIER MONTET, Céntre de Coopération Internationale en Recherche Agronomique pour le Dévelopment. |
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Título: |
Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Food Protection, v. 73, n. 12, p. 2225-2231, 2010. |
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DOI: |
https://doi.org/10.4315/0362-028X-73.12.2225 |
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Idioma: |
Inglês |
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Conteúdo: |
A group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety. MenosA group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high ge... Mostrar Tudo |
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Palavras-Chave: |
Minas frescal cheese. |
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Thesagro: |
Staphylococcus Aureus. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/881434/1/Toxigenic-status-of-Staphylococcus-aureus-isolated-from-bovine.pdf
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Marc: |
LEADER 02530naa a2200253 a 4500
001 1881434
005 2022-08-09
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4315/0362-028X-73.12.2225$2DOI
100 1 $aARCURI, E. F.
245 $aToxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil.$h[electronic resource]
260 $c2010
520 $aA group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety.
650 $aStaphylococcus Aureus
653 $aMinas frescal cheese
700 1 $aÂNGELO, F. F.
700 1 $aTALON, R.
700 1 $aBORGES, M. de F.
700 1 $aLEROY, S.
700 1 $aLOISEAU, G.
700 1 $aLANGE, C. C.
700 1 $aANDRADE, N. J. de
700 1 $aMONTET, D.
773 $tJournal of Food Protection$gv. 73, n. 12, p. 2225-2231, 2010.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 148 | |
| 8. |  | ÂNGELO, F. F.; ARCURI, E. F.; ANDRADE, N. J.; MERHI, C. M.; LANGE, C. C. Avaliação da presença de genes para enterotoxinas, pela técnica de PCR multiplex, em Staphylococcus aureus isolados de mastite bovina na Zona da Mata mineira. In: CONGRESSO NACIONAL DE LATICÍNIOS, 24., 2007, Juiz de Fora. Anais... Belo Horizonte: EPAMIG, 2007. p. 410-413.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | LANGE, C. C.; NOGUEIRA, J. S.; VICENTINI, N. M.; DINIZ, F. H.; PIRES, M. de F. A. Enumeração de estafilococos e coliformes em queijo artesanal produzido no município de Carvalhos, Minas Gerais. In: CONGRESSO NACIONAL DE LATICÍNIOS, 32., 2019, Juiz de Fora. Resumos... Belo Horizonte: EPAMIG, 2019. p. 37.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 19. | | CAVALCANTI, A. C.; VIRGULINO, M. S.; MATOS, L. de; SOARES, A. R. M.; LANGE, C. C.; DIAS, J. A. Epidemiologia da mastite clínica em rebanho de leite tecnificado do estado de Rondônia. In: ENCONTRO DE INICIAÇÃO À PESQUISA, 13.; ENCONTRO DE PÓS-GRADUAÇÃO DA EMBRAPA RONDÔNIA, 8., 2023, Porto Velho. Inovações Metodológicas para Pesquisa Agropecuária: Anais. Porto Velho: Embrapa Rondônia, 2023. p. 22. (Embrapa Rondônia. Eventos Técnicos & Científicos, 01).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| Registros recuperados : 148 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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