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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
05/05/2023 |
Data da última atualização: |
14/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAMPOS, J. da S.; VITAL JÚNIOR, A. C.; BONIEK, D.; SILVA, D. L. da; LANA, U. G. de P.; FERNANDES, J. V.; STOIANOFF, M. A. de R.; ANDRADE, V. S. |
Afiliação: |
JÉSSICA DA SILVA CAMPOS, Universidade Federal Do Rio Grande Do Norte; ANTONIO CARLOS VITAL JÚNIOR, Universidade Federal Do Rio Grande Do Norte; DOUGLAS BONIEK, Universidade Federal de Minas Gerais; DANIELLE LETICIA DA SILVA, Universidade Federal de Minas Gerais; UBIRACI GOMES DE PAULA LANA, CNPMS; JOSÉ VERÍSSIMO FERNANDES, Universidade Federal Do Rio Grande Do Norte; MARIA APARECIDA DE RESENDE STOIANOFF, Universidade Federal de Minas Gerais; VÂNIA SOUSA ANDRADE, Universidade Federal Do Rio Grande Do Norte. |
Título: |
Identification and evaluation of thermotolerance of yeasts from milk in natura exposed to high temperature and slow and fast pasteurization. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 54, p. 1075-1082, 2023. |
DOI: |
https://doi.org/10.1007/s42770-023-00975-7 |
Idioma: |
Inglês |
Conteúdo: |
Milk is considered one of the basic raw materials of animal origin; it must present hygienic quality and physical?chemical properties suitable for processing and human consumption. Thus, the ingestion of milk in natura when not properly treated can be characterized as an opportunistic route of transmission of possible microbial pathogens, which can offer risks to public health. The present study aimed the yeast identification, to analyze the thermo-resistance of yeasts isolated from fresh milk, and to trace the susceptibility profile of the isolates to antifungal agents. For this, 23 samples of fresh milk type B, collected by manual or mechanical milking, were stored in collective refrigeration tanks of farms located in the Metropolitan Region of Natal and nearby, State of Rio Grande do Norte (RN), Brazil. Twenty samples of fresh milk commercially traded in the city of Ceará-Mirim RN were also analyzed. The yeasts were quantified by count of colony-forming units (CFU). All isolated species were treated by slow pasteurization (62?64 °C for 30 min) and fast (72?75 °C for 20 s), as well as by boiling (100 °C). Fifty yeast strains were obtained, and the species were identified as Candida tropicalis (28%), Candida parapsilosis (14%), Candida albicans (12%), Candida glabrata (10%), Candida krusei (10%), Kluyveromyces marxianus (10%), Candida guilliermondii (8%), Candida rugosa (2%), Candida orthopsilosis (2%), Pichia manshurica (2%), and Kodamaea ohmeri (2%). Five isolates showed resistance to the antifungal agents tested. Among all the isolates submitted to heat treatment, 80% were resistant to fast pasteurization and 60% to boiling, but none of them resisted the slow pasteurization. The milk collected through mechanical milking and stored in collective cooling tanks, presented higher rates of yeast contamination, compared to milk samples collected by manual milking and kept under the same storage conditions. MenosMilk is considered one of the basic raw materials of animal origin; it must present hygienic quality and physical?chemical properties suitable for processing and human consumption. Thus, the ingestion of milk in natura when not properly treated can be characterized as an opportunistic route of transmission of possible microbial pathogens, which can offer risks to public health. The present study aimed the yeast identification, to analyze the thermo-resistance of yeasts isolated from fresh milk, and to trace the susceptibility profile of the isolates to antifungal agents. For this, 23 samples of fresh milk type B, collected by manual or mechanical milking, were stored in collective refrigeration tanks of farms located in the Metropolitan Region of Natal and nearby, State of Rio Grande do Norte (RN), Brazil. Twenty samples of fresh milk commercially traded in the city of Ceará-Mirim RN were also analyzed. The yeasts were quantified by count of colony-forming units (CFU). All isolated species were treated by slow pasteurization (62?64 °C for 30 min) and fast (72?75 °C for 20 s), as well as by boiling (100 °C). Fifty yeast strains were obtained, and the species were identified as Candida tropicalis (28%), Candida parapsilosis (14%), Candida albicans (12%), Candida glabrata (10%), Candida krusei (10%), Kluyveromyces marxianus (10%), Candida guilliermondii (8%), Candida rugosa (2%), Candida orthopsilosis (2%), Pichia manshurica (2%), and Kodamaea ohmeri (2%). Five isolates showed ... Mostrar Tudo |
Thesagro: |
Leite; Pasteurização. |
Thesaurus Nal: |
Boiling; Candida; Milk; Pasteurization; Yeasts. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02866naa a2200301 a 4500 001 2153519 005 2023-06-14 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s42770-023-00975-7$2DOI 100 1 $aCAMPOS, J. da S. 245 $aIdentification and evaluation of thermotolerance of yeasts from milk in natura exposed to high temperature and slow and fast pasteurization.$h[electronic resource] 260 $c2023 520 $aMilk is considered one of the basic raw materials of animal origin; it must present hygienic quality and physical?chemical properties suitable for processing and human consumption. Thus, the ingestion of milk in natura when not properly treated can be characterized as an opportunistic route of transmission of possible microbial pathogens, which can offer risks to public health. The present study aimed the yeast identification, to analyze the thermo-resistance of yeasts isolated from fresh milk, and to trace the susceptibility profile of the isolates to antifungal agents. For this, 23 samples of fresh milk type B, collected by manual or mechanical milking, were stored in collective refrigeration tanks of farms located in the Metropolitan Region of Natal and nearby, State of Rio Grande do Norte (RN), Brazil. Twenty samples of fresh milk commercially traded in the city of Ceará-Mirim RN were also analyzed. The yeasts were quantified by count of colony-forming units (CFU). All isolated species were treated by slow pasteurization (62?64 °C for 30 min) and fast (72?75 °C for 20 s), as well as by boiling (100 °C). Fifty yeast strains were obtained, and the species were identified as Candida tropicalis (28%), Candida parapsilosis (14%), Candida albicans (12%), Candida glabrata (10%), Candida krusei (10%), Kluyveromyces marxianus (10%), Candida guilliermondii (8%), Candida rugosa (2%), Candida orthopsilosis (2%), Pichia manshurica (2%), and Kodamaea ohmeri (2%). Five isolates showed resistance to the antifungal agents tested. Among all the isolates submitted to heat treatment, 80% were resistant to fast pasteurization and 60% to boiling, but none of them resisted the slow pasteurization. The milk collected through mechanical milking and stored in collective cooling tanks, presented higher rates of yeast contamination, compared to milk samples collected by manual milking and kept under the same storage conditions. 650 $aBoiling 650 $aCandida 650 $aMilk 650 $aPasteurization 650 $aYeasts 650 $aLeite 650 $aPasteurização 700 1 $aVITAL JÚNIOR, A. C. 700 1 $aBONIEK, D. 700 1 $aSILVA, D. L. da 700 1 $aLANA, U. G. de P. 700 1 $aFERNANDES, J. V. 700 1 $aSTOIANOFF, M. A. de R. 700 1 $aANDRADE, V. S. 773 $tBrazilian Journal of Microbiology$gv. 54, p. 1075-1082, 2023.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
23/10/2001 |
Data da última atualização: |
02/08/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LEAO, P. C. de S.; POSSIDIO, E. L. de; GRANGEIRO, L. C. |
Afiliação: |
PATRICIA COELHO DE SOUZA LEAO, CPATSA; EDSON LUSTOSA DE POSSIDIO, CPATSA. |
Título: |
Avaliação de variedades de uvas sem sementes no Vale do rio São Francisco. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO DE FRUTEIRAS, 2., 2000, Viçosa, MG. Anais... Viçosa, MG: UFV, 2000. |
Páginas: |
p. 175. |
Idioma: |
Português |
Conteúdo: |
Com o objetivo de introduzir, avaliar e selecionar variedades de uva sem semente. |
Palavras-Chave: |
Seedless grapes; Uva sem semente; Vale do Sao Francisco. |
Thesagro: |
Uva. |
Thesaurus NAL: |
Grapes; Varieties. |
Categoria do assunto: |
A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177359/1/Simposio-Brasileiro-de-melhoramento-de-fruteiras-2-2000-p.-175.pdf
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Marc: |
LEADER 00737nam a2200217 a 4500 001 1134354 005 2023-08-02 008 2000 bl uuuu u00u1 u #d 100 1 $aLEAO, P. C. de S. 245 $aAvaliação de variedades de uvas sem sementes no Vale do rio São Francisco. 260 $aIn: SIMPÓSIO BRASILEIRO DE MELHORAMENTO DE FRUTEIRAS, 2., 2000, Viçosa, MG. Anais... Viçosa, MG: UFV$c2000 300 $ap. 175. 520 $aCom o objetivo de introduzir, avaliar e selecionar variedades de uva sem semente. 650 $aGrapes 650 $aVarieties 650 $aUva 653 $aSeedless grapes 653 $aUva sem semente 653 $aVale do Sao Francisco 700 1 $aPOSSIDIO, E. L. de 700 1 $aGRANGEIRO, L. C.
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