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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/10/2021 |
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Data da última atualização: |
03/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COTTA, S. P. M.; MARINS, M. S.; MARRIEL, I. E.; LANA, U. G. de P.; GOMES, E. A.; FIGUEIREDO, J. E. F.; OLIVEIRA-PAIVA, C. A. |
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Afiliação: |
Centro Universitário de Sete Lagoas, UNIFEMM; Universidade Federal de Lavras; IVANILDO EVODIO MARRIEL, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; ELIANE APARECIDA GOMES, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS. |
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Título: |
Thermo-resistant enzyme-producing microorganisms isolated from composting. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Brazilian Journal of Biology, v. 83, e244205, 2023. |
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Idioma: |
Inglês |
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Notas: |
Publicado em: 27 mar. 2021. |
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Conteúdo: |
Organo-mineral fertilizers supplemented with biological additives are an alternative to chemical fertilizers. In this study, thermoresistant microorganisms from composting mass were isolated by two-step procedures. First, samples taken at different time points and temperatures (33 days at 52 ºC, 60 days at 63 ºC, and over 365 days at 26 ºC) were pre-incubated at 80o C for 30 minutes. Second, the microbial selection by in vitro culture-based methods and heat shock at 60o C and 100o C for 2h and 4h. Forty-one isolates were able to grow at 60 °C for 4h; twenty-seven at 100 °C for 2h, and two at 100 °C for 4h. The molecular identification by partial sequencing of the 16S ribosomal gene using universal primers revealed that thirty-five isolates were from eight Bacillus species, one Brevibacillus borstelensis, three Streptomyces thermogriseus, and two fungi (Thermomyces lanuginosus and T. dupontii). Data from amylase, phytase, and cellulase activity assays and the enzymatic index (EI) showed that 38 of 41 thermo-resistant isolates produce at least one enzyme. For amylase activity, the highest EI value was observed in Bacillus licheniformis (isolate 21C2, EI= 4.11), followed by Brevibacillus borstelensis (isolate 6C2, EI= 3.66), Bacillus cereus (isolate 18C2, EI= 3.52), and Bacillus paralicheniformis (isolate 20C2, EI= 3.34). For phytase, the highest EI values were observed for Bacillus cereus (isolate 18C2, EI= 2.30) and Bacillus licheniformis (isolate 3C1, EI= 2.15). Concerning cellulose production, B. altitudinis (isolate 6C1) was the most efficient (EI= 6.40), followed by three Bacillus subtilis (isolates 9C1, 16C2, and 19C2) with EI values of 5.66, 5.84, and 5.88, respectively, and one B. pumilus (isolate 27C2, EI= 5.78). The selected microorganisms are potentially useful as a biological additive in organo-mineral fertilizers and other biotechnological processes. MenosOrgano-mineral fertilizers supplemented with biological additives are an alternative to chemical fertilizers. In this study, thermoresistant microorganisms from composting mass were isolated by two-step procedures. First, samples taken at different time points and temperatures (33 days at 52 ºC, 60 days at 63 ºC, and over 365 days at 26 ºC) were pre-incubated at 80o C for 30 minutes. Second, the microbial selection by in vitro culture-based methods and heat shock at 60o C and 100o C for 2h and 4h. Forty-one isolates were able to grow at 60 °C for 4h; twenty-seven at 100 °C for 2h, and two at 100 °C for 4h. The molecular identification by partial sequencing of the 16S ribosomal gene using universal primers revealed that thirty-five isolates were from eight Bacillus species, one Brevibacillus borstelensis, three Streptomyces thermogriseus, and two fungi (Thermomyces lanuginosus and T. dupontii). Data from amylase, phytase, and cellulase activity assays and the enzymatic index (EI) showed that 38 of 41 thermo-resistant isolates produce at least one enzyme. For amylase activity, the highest EI value was observed in Bacillus licheniformis (isolate 21C2, EI= 4.11), followed by Brevibacillus borstelensis (isolate 6C2, EI= 3.66), Bacillus cereus (isolate 18C2, EI= 3.52), and Bacillus paralicheniformis (isolate 20C2, EI= 3.34). For phytase, the highest EI values were observed for Bacillus cereus (isolate 18C2, EI= 2.30) and Bacillus licheniformis (isolate 3C1, EI= 2.15). Concerning c... Mostrar Tudo |
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Palavras-Chave: |
Microrganismo termoresistente. |
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Thesagro: |
Enzima; Fertilizante; Microrganismo. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227293/1/Thermo-resistant.pdf
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Marc: |
LEADER 02644naa a2200253 a 4500
001 2135666
005 2022-02-03
008 2023 bl uuuu u00u1 u #d
100 1 $aCOTTA, S. P. M.
245 $aThermo-resistant enzyme-producing microorganisms isolated from composting.$h[electronic resource]
260 $c2023
500 $aPublicado em: 27 mar. 2021.
520 $aOrgano-mineral fertilizers supplemented with biological additives are an alternative to chemical fertilizers. In this study, thermoresistant microorganisms from composting mass were isolated by two-step procedures. First, samples taken at different time points and temperatures (33 days at 52 ºC, 60 days at 63 ºC, and over 365 days at 26 ºC) were pre-incubated at 80o C for 30 minutes. Second, the microbial selection by in vitro culture-based methods and heat shock at 60o C and 100o C for 2h and 4h. Forty-one isolates were able to grow at 60 °C for 4h; twenty-seven at 100 °C for 2h, and two at 100 °C for 4h. The molecular identification by partial sequencing of the 16S ribosomal gene using universal primers revealed that thirty-five isolates were from eight Bacillus species, one Brevibacillus borstelensis, three Streptomyces thermogriseus, and two fungi (Thermomyces lanuginosus and T. dupontii). Data from amylase, phytase, and cellulase activity assays and the enzymatic index (EI) showed that 38 of 41 thermo-resistant isolates produce at least one enzyme. For amylase activity, the highest EI value was observed in Bacillus licheniformis (isolate 21C2, EI= 4.11), followed by Brevibacillus borstelensis (isolate 6C2, EI= 3.66), Bacillus cereus (isolate 18C2, EI= 3.52), and Bacillus paralicheniformis (isolate 20C2, EI= 3.34). For phytase, the highest EI values were observed for Bacillus cereus (isolate 18C2, EI= 2.30) and Bacillus licheniformis (isolate 3C1, EI= 2.15). Concerning cellulose production, B. altitudinis (isolate 6C1) was the most efficient (EI= 6.40), followed by three Bacillus subtilis (isolates 9C1, 16C2, and 19C2) with EI values of 5.66, 5.84, and 5.88, respectively, and one B. pumilus (isolate 27C2, EI= 5.78). The selected microorganisms are potentially useful as a biological additive in organo-mineral fertilizers and other biotechnological processes.
650 $aEnzima
650 $aFertilizante
650 $aMicrorganismo
653 $aMicrorganismo termoresistente
700 1 $aMARINS, M. S.
700 1 $aMARRIEL, I. E.
700 1 $aLANA, U. G. de P.
700 1 $aGOMES, E. A.
700 1 $aFIGUEIREDO, J. E. F.
700 1 $aOLIVEIRA-PAIVA, C. A.
773 $tBrazilian Journal of Biology$gv. 83, e244205, 2023.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
23/11/2018 |
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Data da última atualização: |
23/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
COSTA, A. M.; MOTOIKE, S. Y.; CORRÊA, T. R.; SILVA, T. C.; COSER, S. M.; RESENDE, M. D. V. de; TEÓFILO, R. F. |
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Afiliação: |
Annanda Mendes Costa, UFV; Sérgio Yoshimitsu Motoike, UFV; Thais Roseli Corrêa, UFV; Thaís Cristina Silva, UFV; Sara Morra Coser, UFV; MARCOS DEON VILELA DE RESENDE, CNPF; Reinaldo Francisco Teófilo, UFV. |
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Título: |
Genetic parameters and selection of macaw palm (Acrocomia aculeata) accessions: an alternative crop for biofuels. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 18, N. 3, p. 259-266, July/Sept. 2018. |
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DOI: |
10.1590/1984- 70332018v18n3a39 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to estimate the parameters related to the genetic control of the physical characteristics of the fruits, oil content, and oil yield, and to proceed with the selection of macaw palm accessions. Forty-four macaw palm accessions of the active germplasm collection of the Federal University of Viçosa were collected for the evaluation of epicarp dry matter, pulp dry matter, endocarp dry matter, kernel dry matter, oil content, and oil yield. Narrow-sense individual heritability estimates were considered as of intermediate magnitude. The coefficient of repeatability and the accuracy in family selection was of high magnitude. Oil yield per plant presented the highest coefficient of individual genetic variation. Five different accessions contributed to the ten first individuals selected by the individual BLUP. The Mulamba and Mock?s rank index allowed classifying the accessions in a sequence favorable to selection. |
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Palavras-Chave: |
Genetic variability; Macaw palm; Variabilidade genética. |
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Thesagro: |
Acrocomia Aculeata; Bioenergia; Espécie Nativa; Macaúba. |
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Thesaurus NAL: |
Arecaceae; Bioenergy. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186845/1/2018-M.Deon-CBAB-Genetic.pdf
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Marc: |
LEADER 01892naa a2200313 a 4500
001 2099924
005 2018-11-23
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1590/1984- 70332018v18n3a39$2DOI
100 1 $aCOSTA, A. M.
245 $aGenetic parameters and selection of macaw palm (Acrocomia aculeata) accessions$ban alternative crop for biofuels.$h[electronic resource]
260 $c2018
520 $aThis study aimed to estimate the parameters related to the genetic control of the physical characteristics of the fruits, oil content, and oil yield, and to proceed with the selection of macaw palm accessions. Forty-four macaw palm accessions of the active germplasm collection of the Federal University of Viçosa were collected for the evaluation of epicarp dry matter, pulp dry matter, endocarp dry matter, kernel dry matter, oil content, and oil yield. Narrow-sense individual heritability estimates were considered as of intermediate magnitude. The coefficient of repeatability and the accuracy in family selection was of high magnitude. Oil yield per plant presented the highest coefficient of individual genetic variation. Five different accessions contributed to the ten first individuals selected by the individual BLUP. The Mulamba and Mock?s rank index allowed classifying the accessions in a sequence favorable to selection.
650 $aArecaceae
650 $aBioenergy
650 $aAcrocomia Aculeata
650 $aBioenergia
650 $aEspécie Nativa
650 $aMacaúba
653 $aGenetic variability
653 $aMacaw palm
653 $aVariabilidade genética
700 1 $aMOTOIKE, S. Y.
700 1 $aCORRÊA, T. R.
700 1 $aSILVA, T. C.
700 1 $aCOSER, S. M.
700 1 $aRESENDE, M. D. V. de
700 1 $aTEÓFILO, R. F.
773 $tCrop Breeding and Applied Biotechnology$gv. 18, N. 3, p. 259-266, July/Sept. 2018.
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Embrapa Florestas (CNPF) |
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