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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
12/08/2019 |
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Data da última atualização: |
24/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, G. P.; LOPES, B. A.; MARRIEL, I. E.; LANA, U. G. de P.; TAKAHASHIA, J. A. |
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Afiliação: |
Geane P. Oliveira, Universidade Fedral de Minas Gerais; Barbara O. Lopes, Universidade Fedral de Minas Gerais; IVANILDO EVODIO MARRIEL, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; Jacqueline A. Takahashia, Universidade Fedral de Minas Gerais. |
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Título: |
Design of intelligent molecules as models for production of new anti-Alzheimer's medicines using epigenetic modulation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Chemical Engineering Transactions, v. 74, p. 1561-1566, 2019. |
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DOI: |
10.3303/CET1974261 |
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Idioma: |
Inglês |
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Conteúdo: |
Alzheimer's disease is a neurodegenerative illness, so far without cure, that affects an increasing number of people around the world. Due to the adverse effects caused by drugs for treating this disease currently in clinical use, it is necessary to search for more effective and less aggressive therapeutic resources. In this work a modern technological alternative was used to build new drugs useful for the treatment of Alzheimer's disease from fungi. This strategy consists on the development of pools of fungal molecules able of act in intelligent synergistic mechanisms to block acetylcholinesterase, enzyme involved in the development of Alzheimer's disease. Expression of pools containing new secondary metabolites can be achieved by adding DNA methyltransferase inhibitors to the fermentative process. This fast in vitro approach was used to build a combination of fungal metabolites containing acetylcholinesterase inhibitors. Biosynthesis elicitation was achieved by adding DNA methyltransferases inhibitors (hydralazine and procainamide) to solid culture media and then filamentous fungi Aspergillus chevalieri, Talaromyces calidicanius, Clonostachys rogersoniana, Fusarium nygamai, and Penicillium sp. were added. Straightforward HPLC analysis showed elicitation of new compounds by all species, being hydralazine the most effective epigenetic modifier especially towards T. calidicanius. Procainamide was able to enable expression of several major new secondary metabolites as for A. chevalieri (RT 51 min), and F. nygamai (RT 21.5 min). In general, the pools of molecules expressed after epigenetic modulation were more effective in acetylcholinesterase inhibition than the metabolites expressed without modulation. Modulation of C. rogersoniana by procainamide increased approximately four times the biosynthesis of antiacetylcholinesterase metabolites in relation to the control. T. calidicanius and Penicillium species modulated with procainamide produced a pool of metabolites about twice more active in regarding to the basal metabolome, while the pool of metabolites from C. rogersoniana and T. calidicanius were five and three times more active (40.34 ± 0.15% and 48.54 ± 1.34%, respectively) than the non-modulated extracts. MenosAlzheimer's disease is a neurodegenerative illness, so far without cure, that affects an increasing number of people around the world. Due to the adverse effects caused by drugs for treating this disease currently in clinical use, it is necessary to search for more effective and less aggressive therapeutic resources. In this work a modern technological alternative was used to build new drugs useful for the treatment of Alzheimer's disease from fungi. This strategy consists on the development of pools of fungal molecules able of act in intelligent synergistic mechanisms to block acetylcholinesterase, enzyme involved in the development of Alzheimer's disease. Expression of pools containing new secondary metabolites can be achieved by adding DNA methyltransferase inhibitors to the fermentative process. This fast in vitro approach was used to build a combination of fungal metabolites containing acetylcholinesterase inhibitors. Biosynthesis elicitation was achieved by adding DNA methyltransferases inhibitors (hydralazine and procainamide) to solid culture media and then filamentous fungi Aspergillus chevalieri, Talaromyces calidicanius, Clonostachys rogersoniana, Fusarium nygamai, and Penicillium sp. were added. Straightforward HPLC analysis showed elicitation of new compounds by all species, being hydralazine the most effective epigenetic modifier especially towards T. calidicanius. Procainamide was able to enable expression of several major new secondary metabolites as for A. c... Mostrar Tudo |
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Palavras-Chave: |
Doença neurodegenerativa; Moléculas fúngicas. |
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Thesagro: |
Doença; Fungo; Medicamento. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/200581/1/Design-intelligent.pdf
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Marc: |
LEADER 02999naa a2200241 a 4500
001 2111258
005 2020-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3303/CET1974261$2DOI
100 1 $aOLIVEIRA, G. P.
245 $aDesign of intelligent molecules as models for production of new anti-Alzheimer's medicines using epigenetic modulation.$h[electronic resource]
260 $c2019
520 $aAlzheimer's disease is a neurodegenerative illness, so far without cure, that affects an increasing number of people around the world. Due to the adverse effects caused by drugs for treating this disease currently in clinical use, it is necessary to search for more effective and less aggressive therapeutic resources. In this work a modern technological alternative was used to build new drugs useful for the treatment of Alzheimer's disease from fungi. This strategy consists on the development of pools of fungal molecules able of act in intelligent synergistic mechanisms to block acetylcholinesterase, enzyme involved in the development of Alzheimer's disease. Expression of pools containing new secondary metabolites can be achieved by adding DNA methyltransferase inhibitors to the fermentative process. This fast in vitro approach was used to build a combination of fungal metabolites containing acetylcholinesterase inhibitors. Biosynthesis elicitation was achieved by adding DNA methyltransferases inhibitors (hydralazine and procainamide) to solid culture media and then filamentous fungi Aspergillus chevalieri, Talaromyces calidicanius, Clonostachys rogersoniana, Fusarium nygamai, and Penicillium sp. were added. Straightforward HPLC analysis showed elicitation of new compounds by all species, being hydralazine the most effective epigenetic modifier especially towards T. calidicanius. Procainamide was able to enable expression of several major new secondary metabolites as for A. chevalieri (RT 51 min), and F. nygamai (RT 21.5 min). In general, the pools of molecules expressed after epigenetic modulation were more effective in acetylcholinesterase inhibition than the metabolites expressed without modulation. Modulation of C. rogersoniana by procainamide increased approximately four times the biosynthesis of antiacetylcholinesterase metabolites in relation to the control. T. calidicanius and Penicillium species modulated with procainamide produced a pool of metabolites about twice more active in regarding to the basal metabolome, while the pool of metabolites from C. rogersoniana and T. calidicanius were five and three times more active (40.34 ± 0.15% and 48.54 ± 1.34%, respectively) than the non-modulated extracts.
650 $aDoença
650 $aFungo
650 $aMedicamento
653 $aDoença neurodegenerativa
653 $aMoléculas fúngicas
700 1 $aLOPES, B. A.
700 1 $aMARRIEL, I. E.
700 1 $aLANA, U. G. de P.
700 1 $aTAKAHASHIA, J. A.
773 $tChemical Engineering Transactions$gv. 74, p. 1561-1566, 2019.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 223 | |
| 24. |  | JARDIM, S. N.; SOUZA, I. R. P. de; GUIMARAES, C. T.; SABATO, E. de O.; LANA, U. G. de P. Avaliação da resistência ao mosaico comum em milho em função do tempo após a inoculação. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 5., 2009, Vitória. O melhoramento e os novos cenários da agricultura: anais. Vitória: Incaper, 2009. 1 CD-ROM. (Incaper. Documentos, 11).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 26. | | GOMES, E. A.; GUIMARAES, C. T.; LANA, U. G. de P.; VERDOLIN, A. L.; OLIVEIRA-PAIVA, C. A. Avaliação do impacto de milho transgênico expressando o gene de tolerância ao alumínio SbMATE sobre a comunidade bacteriana do solo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 31., 2016, Bento Gonçalves. Milho e sorgo: inovações, mercados e segurança alimentar: anais. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 27. | | VALICENTE, F. H.; PICOLI, E.; VASCONCELOS, M.; CARNEIRO, N.; CARNEIRO, A.; GUIMARAES, C. T.; LANA, U. G. de P. Characterization, distribution and cloning cry1 genes efficient against fall armyworm, Spodoptera frugiperda, in Brazil. In: ANNUAL MEETING SOCIETY FOR INVERTEBRATE PATHOLOGY, 42., 2009, Park City, Utah. Program and abstracts. Park City: Society Invertebrate Pathology, 2009. p. 107| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 28. | | LANA, U. G. de P.; GUIMARAES, C. T.; SOUZA, I. R. P. de; BELICUAS, S. N. J. Desenvolvimento de marcadores moleculares baseados em análogos de genes de resistência associados a QTLs de resistência à mancha-branca-do-milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 31. | | SILVA, U. de C.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; GOMES, E. A.; SANTOS, V. L. dos. Growth promotion features of the maize microbiome: from an agriculture perspective. In: CHOUDHARY, D. K.; VARMA, A.; TUTEJA, N. (Ed.). Plant-microbe interaction: an approach to sustainable agriculture. Singapore: Springer, 2016. cap. 16, p. 345-374.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 32. | | VALICENTE, F. H.; LANA, U. G. de P.; PEREIRA, A. C. P.; MARTINS, J. L. A.; TAVARES, A. N. G. Base científica dos modelos de "agricultura fermentativa" disseminados pelo país: riscos e oportunidades. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIAS DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA - RELARE, 19., 2018, Foz do Iguaçu. Anais... Foz do Iguaçu: Embrapa, 2018. p. 16.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 34. | | GOMES, E. A.; SOARES, F. A. de O.; LANA, U. G. de P.; SOUZA, F. A. de. Caracterização molecular de fungos micorrízicos arbusculares associados a genótipos de milho contrastantes para eficiência no uso de fósforo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2010. FertBio 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 37. | | GOMES, E. A.; LANA, U. G. de P.; OLIVEIRA, F. A. S. de; SOUZA, F. A. de. Diversidade de fungos micorrízicos arbusculares associada às raízes de duas linhagens de milho contrastantes para eficiência no uso de fósforo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 38. | | GONÇALVES, A. B. da S.; GOMES, E. A.; LANA, U. G. de P.; SOUSA, S. M. de; GUIMARAES, C. T. Estabelecimento de metodologia para análise e quantificação da colonização por fungo micorrízico arbuscular em milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 31., 2016, Bento Gonçalves. Milho e sorgo: inovações, mercados e segurança alimentar: anais. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 40. | | RABEL, M.; VIEIRA, E. S. N.; LANA, U. G. de P.; PAIVA, E.; SEHNEM, M. A. S.; SCHUSTER, I. Marcadores moleculares microssatélites na avaliação de sementes de soja com variação na coloração do hilo. Revista Brasileira de Sementes, Londrina, v. 32, n. 2, p. 19-25, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 223 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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