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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/12/1992 |
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Data da última atualização: |
09/04/2009 |
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Autoria: |
LAGUERRE, G.; MAZURIER, S. I.; AMARGER, N. |
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Afiliação: |
Institut National de la Recherche Agronomique, Laboratoire de Microbiologie des Sols, 17 rue Sully, BV 1540, 21034 Dijon Cedex, France. |
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Título: |
Plasmid profiles and restriction fragment length polymorphism of Rhizobium leguminosarum bv. viciae in field populations. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
FEMS Microbiology Ecology, v.101, p.17-26, 1992. |
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Idioma: |
Inglês |
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Conteúdo: |
56 isolates of Rhizobium leguminosarum biovar viciae from one field were characterized by analysis of plasmid profile, total DNA restriction pattern and restriction fragment length polymorphism (RFLP) of 2 chromosomal regions and of symbiotic (Sym) plasmid. Different levels of similarity exist in patterns generated by the different techniques. At the level of partial similarity these techniques give comparable results for more than 80% of the isolates, with the exception of RFLP profiling with the Sym probe. Analysis at this level allows the grouping of the isolates that have most of their non-Sym genome similarity, the techniques are no more equivalent and provide complementary information on possible evolution of the different elements of the genome identified by each specific technique. The non-Sym plasmids defining classes were strongly associated with specific chromosomal backgrounds. In contrast, variations in Sym plasmids were not related with variations in the remaining genome. Host range towards chromosomes was variable among the Sym plasmids, which may reflect plasmid transfer between strains. |
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Palavras-Chave: |
Diversidade genetica; DNA fingerprint; Genetic diversity; Length polymorphism; Methology; Metodologia; Perfil; Plasmid profiles; Restriction fragment; RFLP; Symbiotic plasmid. |
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Thesagro: |
Plasmídeo; Rhizobium Leguminosarum. |
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Categoria do assunto: |
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Marc: |
LEADER 01980naa a2200301 a 4500
001 1457875
005 2009-04-09
008 1992 bl --- 0-- u #d
100 1 $aLAGUERRE, G.
245 $aPlasmid profiles and restriction fragment length polymorphism of Rhizobium leguminosarum bv. viciae in field populations.
260 $c1992
520 $a56 isolates of Rhizobium leguminosarum biovar viciae from one field were characterized by analysis of plasmid profile, total DNA restriction pattern and restriction fragment length polymorphism (RFLP) of 2 chromosomal regions and of symbiotic (Sym) plasmid. Different levels of similarity exist in patterns generated by the different techniques. At the level of partial similarity these techniques give comparable results for more than 80% of the isolates, with the exception of RFLP profiling with the Sym probe. Analysis at this level allows the grouping of the isolates that have most of their non-Sym genome similarity, the techniques are no more equivalent and provide complementary information on possible evolution of the different elements of the genome identified by each specific technique. The non-Sym plasmids defining classes were strongly associated with specific chromosomal backgrounds. In contrast, variations in Sym plasmids were not related with variations in the remaining genome. Host range towards chromosomes was variable among the Sym plasmids, which may reflect plasmid transfer between strains.
650 $aPlasmídeo
650 $aRhizobium Leguminosarum
653 $aDiversidade genetica
653 $aDNA fingerprint
653 $aGenetic diversity
653 $aLength polymorphism
653 $aMethology
653 $aMetodologia
653 $aPerfil
653 $aPlasmid profiles
653 $aRestriction fragment
653 $aRFLP
653 $aSymbiotic plasmid
700 1 $aMAZURIER, S. I.
700 1 $aAMARGER, N.
773 $tFEMS Microbiology Ecology$gv.101, p.17-26, 1992.
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Registro original: |
Embrapa Soja (CNPSO) |
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