Data/hora: 16/06/2024 05:58:09

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 16/02/2017
Data da última atualização: | 30/01/2023
Autoria: | MUNK, M.; LADEIRA, L. O.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; RAPOSO, N. R. B.; SERAPIÁO, R. V.; QUINTAO, C. C. R.; SILVA, S. R.; SOARES, J. S.; JORIO, A.; BRANDAO, H. de M.
Afiliação: | Michele Munk, UFJF; Luiz O. Ladeira, UFMG; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Nádia R. B. Raposo, UFJF; Raquel V. Serapião, PESAGRO; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; Saulo R. Silva; Jaqueline S. Soares, UFOP; Ado Jorio, UFMG; HUMBERTO DE MELLO BRANDAO, CNPGL.
Título: | Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.
Ano de publicação: | 2016
Fonte/Imprenta: | Nature, Scientific Reports, v. 6, article 33588, 2016.
Idioma: | Inglês
Conteúdo: | The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.
Palavras-Chave: | Gene delivery; Pellucid zone.
Thesaurus Nal: | in vitro fertilization.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/155955/1/Cnpgl-2016-Nature-Efficient.pdf
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (23350-1) - UPC - AP - DD -  -  -  - DIS