Data/hora: 12/06/2024 07:58:07

Biblioteca(s): | Embrapa Suínos e Aves.
Data corrente: | 10/08/2021
Data da última atualização: | 10/08/2021
Autoria: | ROMERO TISOT, C. M.; ZANELLA, R.; SOUZA, A. P.; LÖF, L. M.; SOARES, J. C. M.; MARQUES, M. G.; ZANELLA, E. L.
Afiliação: | CÂNDIDA MALDANER ROMERO TISOT, UPF; RICARDO ZANELLA, UPF; UDESC/Lages; LUCAS MALLMANN LÖF, UPF; UFSM; MARIANA GROKE MARQUES, CNPSA; ERALDO LOURENSO ZANELLA, UPF.
Título: | Melatonin effect on cryopreserved sperm cells of crioulo stallions.
Ano de publicação: | 2020
Fonte/Imprenta: | Open Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020.
DOI: | 10.23880/oajvsr-16000202
Idioma: | Inglês
Conteúdo: | Abstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation.
Palavras-Chave: | Melatonina.
Thesagro: | Criopreservação; Espermatozóide; Garanhão; Reprodução Animal.
Thesaurus Nal: | Animal reproduction; Cryopreservation; Melatonin; Reactive oxygen species; Spermatozoa; Stallions.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/225036/1/final9560.pdf
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (22177-1) - UPC - AP - DD -  -  -  - DIS