Data/hora: 02/06/2024 23:58:42

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	17/05/1993	
Data da última atualização: 	17/05/1993	
Autoria: 	LOPEZ, M. M.; NAVARRO, L.	
Afiliação: 	INIA. Centro de Levante (CRIDA 07). Moncada, Valencia, Spain.	
Título: 	A new in vitro inoculation method for citrus canker diabnosis	
Ano de publicação: 	0	
Fonte/Imprenta: 	Tokyo, Japan: International Society of Citricultural, l981.	
Volume: 	v.1	
Páginas: 	p.399-402	
Idioma: 	Inglês	
Conteúdo: 	Twenty-day-old 'Marsh' grapefruit (Citrus paradisi Macf.) and 'Parson Brown' sweet orange [C. sinensis (L.) osbeck] seedlings growing under sterile conditions in Murashige and Skoog salt solutioons were inoculated by placing a drop of Xanthomonas campestris pv. citri suspension on wounded and unwounded leaves. Inoculated seedlings were incubated at 27 degree C under 16 hr daily exposure to 1,000 lux illumination. Seven to ten days following inoculation, typical symptoms of citrus canker were observed in all inoculated plants. Disease expression appeared sooner and was more severe in wounded than in unwounded plants. Symptoms were not observed in plants inoculated with a strain of Pseudomanas syringae isolated from citrus or with Xamthomonas spp. pathogenic tonon-citrus hosts. Advantages and disadvantages of the method are discussed.	
Thesaurus Nal: 	quarantine; tissue culture.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (13057-1) - ADD - PL - -- - 634.3040601 - I61p - 1992000203 - DIS